A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Recombinant construct P{WT48}

General Information
Symbol P{WT48} FlyBase ID FBtp0000687
Feature type transgenic_transposon
Size Expression data
Associated insertions 0 available
Molecular map
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Description
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FB2013_03
FB2013_02
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hide Description & Uses
Species synthetic construct
Location-dependent
role
Description
CV term
Qualifiers & info
Reference
transposon
P-element
Uses
CV term
Qualifiers & info
Reference
characterization
Cloning Sites
Location
Restriction sites
Reference
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Sequence (FB)
Associated Sequence Data
DDBJ /
EMBL /
GenBank
DNA sequence
Extent
 
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Total Size
Left end
Right end
Segments
Number
Orientation
Symbol
Reference
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CV term
Qualifiers & info
Reference
partial
selectable
hide Component Alleles
Allele twiT48
Reference(s) (Thisse et al., 1991)
Molecular data
Construct: Genomic fragment including twi sequences from -1.4kb to +3.2kb.
Phenotypic class
Phenotype manifest in
Other information
Allele w+mW.hs
Reference(s) (Thisse et al., 1991)
Molecular data
A mini-w gene is expressed under the control of an Hsp70 promoter.
w gene expression is under the influence of FLIP recombinase.
Expression of a fragment of w genomic DNA with a 2.2kb deletion of 3' flanking sequences and a reduction in the size of the largest intron to 356bp is driven by a 388bp fragment of an Hsp70 gene.
Construct: A 'mini-white' gene constructed by deletion of the XbaI-HindII fragment from the long 5' intron. Carried by the W6 and W8 series of vectors.
 
Phenotypic class
Phenotype manifest in
Other information
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Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects expression of
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progenitor(s)
descendant(s)
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construct_comment: One of a series of twi rescue constructs.
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Other Crossreferences
Linkouts
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Reported As
Symbol Synonym
P{WT48}
Secondary FlyBase IDs
hide References ( 1 )
Research paper
Thisse et al., 1991, Cell 65: 1191--1201
Sequence-specific transactivation of the Drosophila twist gene by the dorsal gene product. [FBrf0053349]