FB2025_01 , released February 20, 2025
Recombinant Construct: P{BS3.0}
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General Information
Symbol
P{BS3.0}
FlyBase ID
FBtp0000694
Feature type
transgenic_transposable_element
Component Allele(s)
Also Known As
dpp-LacZ, dpp-lacZBS3.0, dpp-lacZ BS3.0, BS3.0, dppZ, dpp-lacZ (BS3.0), dpp-lacZ(BS3.0), dpp:lacZ
Size
Expression Data
lacZ reporter
Associated insertion(s)
Molecular map
Description and Uses
Construct components
Component
Use(s)
Marker(s)
Component allele Ecol\lacZdpp.BS3.0
Transgenic product class
Component
Use(s)
Regulatory region(s)
Encoded product / tool
Description

lacZ expression is driven by fragment from 106.9-116.9kb of dpp, at the disk III-disk II boundary.

12kb EcoRI fragment spanning dpp region disk III--II boundary drives lacZ expression.

10kb EcoRI fragment spanning dpp region disk III--II boundary drives lacZ expression.

A dpp disc enhancer drives lacZ expression.

lacZ expression is controlled by the 3' dpp enhancer.

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
No Assay Recorded
Stage
Tissue/Position (including subcellular localization)
Reference
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Information
Statement
Reference

The dpp reporter is mostly expressed anterior to phosphorylated Mad at the morphogenetic furrow in third instar larval eye discs.

Expression is observed in both the male and female genital discs, and is complementary to that of wg. Expression is excluded from the repressed male primordium in the female genital disc.

The EcollacZdpp.BS3.0 reporter construct reflects wildtype expression of dpp in the imaginal discs. Expression is detected in a stripe along the anterior-posterior boundary of the wing disc and along the morphogenetic furrow in the eye-antennal disc.

Expressed in cells on anterior side of the A/P boundary.

The expression pattern of EcollacZdpp.BS3.0 most closely recapitulates wild-type dpp expression patterns during development.

 
Reporter construct used in assay
Progenitors and Descendants
descendant(s)
Comments

construct_comment: One of a series of lacZ reporter constructs used to characterize the disk 3\' regulatory region of dpp.

Stocks (4)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (43)
Reported As
Symbol Synonym
(Dpp)-lacZ line 3.0
BS3.0 decapentaplegic
BS3.0 dpp LacZ
BS3.0dpp-lacZ BS3.0
P[dpp-lacZ]
P{dpp-lacZ}dpp-lacZ
dpp-LacZ BS3.0
dpp-LacZ<up>BS 3.0</up>
dpp-lacZdpp.BS3.0
dpp-lacZdpp.BS3.0RA
dpp-lacZBS3.0
dpp-lacZ{BS3.0}
dppBS3.0-lacZ
dppBS3.0:LacZ
dpplacZ BS3.0
Secondary FlyBase IDs
    References (254)