Recombinant construct P{BS3.0}

General Information
Symbol	P{BS3.0}	FlyBase ID	FBtp0000694
Feature type	transgenic_transposon

Size		Expression data	lacZ reporter
Associated insertions	2 available
Molecular map
Recent Updates
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Blackman et al., 1991)
Uses	CV term Qualifiers & info Reference characterization dpp \| regulatory (Blackman et al., 1991) reporter construct dpp (Blackman et al., 1991)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference chimeric gene (Blackman et al., 1991) reporter gene (Blackman et al., 1991) selectable marker (Blackman et al., 1991)
Component Alleles
Allele	Ecol\lacZ^dpp.BS3.0
Reference(s)	(Royet and Finkelstein, 1997, Chen et al., 1997, Capdevila et al., 1994, Abzhanov et al., 2001, Chanut et al., 2002, Chern and Choi, 2002, Ghbeish and McKeown, 2002, Gorfinkiel et al., 1999, Collins and Cohen, 2005, Kenyon et al., 2005, Lai et al., 2000, Pimentel and Venkatesh, 2005, Mukherjee et al., 2000, Jang et al., 2003, Wang et al., 1999, Maves and Schubiger, 1998, Yao et al., 1999, Moreno and Morata, 1999, Takeo et al., 2005, Heberlein et al., 1998, Apidianakis et al., 2001, Takahashi et al., 2005, Pickup et al., 2002, Amanai and Jiang, 2001, Dai et al., 2003, Erkner et al., 2002, Katsuyama et al., 2005, Lin et al., 2003, Ghazi et al., 2003, Cavodeassi et al., 2002, Ogden et al., 2006, de Navas et al., 2006, Pfleger et al., 2007, Jia et al., 2005, Gorfinkiel et al., 2003, Smith et al., 1993, Parker et al., 2002, Noureddine et al., 2002, Heberlein et al., 1993, Jia et al., 2002, Collier et al., 2004, Capdevila and Guerrero, 1994, Jia et al., 2003, Strutt et al., 1995, Sanicola et al., 1995, Ou et al., 2002, Bessa et al., 2002, Pan and Rubin, 1995, Martin et al., 1995, Niwa et al., 2004, Lepage et al., 1995, Li et al., 1995, Lee et al., 2002, Estella et al., 2003, Janody et al., 2004, Jarman et al., 1995, Jiang and Struhl, 1995, Heberlein et al., 1995, Cadigan et al., 2002, Belenkaya et al., 2002, Torroja et al., 2004, Mohler et al., 2000, del Alamo Rodriguez et al., 2004, Marenda et al., 2004, Calleja et al., 2000, Johnson et al., 2003, Sedkov et al., 2003, Buratovich and Bryant, 1995, Aza-Blanc et al., 2000, Heanue et al., 1999, Miletich and Limbourg-Bouchon, 2000, Mandal and Lakhotia, 1999, Treisman, 2001, Haines and van Den Heuvel, 2000, Sanchez et al., 2001, Kumar and Moses, 2001, Zeng et al., 2000, Liu et al., 2008, McCartney et al., 2000, Lee et al., 1994, Cho et al., 2000, Glise et al., 2005, Felsenfeld and Kennison, 1995, Kojima et al., 1994, Svendsen et al., 2000, Collins and Treisman, 2000, Maurel-Zaffran and Treisman, 2000, Estrada and Sanchez-Herrero, 2001, Entchev et al., 2000, Teleman and Cohen, 2000, Chanut et al., 2000, Garoia et al., 2000, Certel et al., 2000, Benlali et al., 2000, Azpiazu and Morata, 2000, Baonza et al., 2000, Greenwood and Struhl, 1999, Weigmann and Cohen, 1999, Alves et al., 1998, Kopp et al., 1999, Goto and Hayashi, 1999, Chen et al., 1999, Vervoort et al., 1999, Garoia et al., 2005, Asano and Wharton, 1999, Methot and Basler, 1999, Ho et al., 2005, Jackson et al., 1997, Dominguez and Hafen, 1997, Joulia et al., 2005, Niwa et al., 2000, Zeng and Verheyen, 2004, Strigini and Cohen, 1997, Strutt and Mlodzik, 1997, Song et al., 2000, Sanchez et al., 1997, Casares et al., 1997, Gorfinkiel et al., 1997, Weigmann et al., 1997, Randsholt et al., 2000, Pichaud and Casares, 2000, Newfeld et al., 1997, Jia et al., 2004, Zelhof et al., 1997, Nestoras et al., 1997, Buratovich and Bryant, 1997, Blackman et al., 1991, Tavsanli et al., 2004, Raftery et al., 1991, Yao et al., 2006, Bras-Pereira et al., 2006, Blair, 1992, Nakano et al., 2004, Perez-Garijo et al., 2004, Maschat et al., 1998, Lee and Treisman, 2001, Go et al., 1998, Lee et al., 2001, Saget et al., 1998, Alexandre et al., 1996, Khalsa et al., 1998, Emerald and Roy, 1998, Chanut and Heberlein, 1995, Freeland and Kuhn, 1996, Guillen et al., 1995, Sanchez-Herrero et al., 1996, Nellen et al., 1996, Abu-Shaar and Mann, 1998, Lecuit et al., 1996, Curtiss and Heilig, 1995, Theodosiou et al., 1998, Hazelett et al., 1998, Lee and Treisman, 2001, Mozer, 2001, Treisman et al., 1995, Borod and Heberlein, 1998, Treisman and Rubin, 1995, Ma and Moses, 1995, Kango-Singh et al., 2003, Ingham and Fietz, 1995, Lukacsovich et al., 2003, Simmonds et al., 1995, Strutt and Mlodzik, 1995, Weatherbee et al., 1998, Treisman et al., 1997, Schneider and Spradling, 1997, Strutt and Mlodzik, 1996, Adamson and Shearn, 1996, Lecuit and Cohen, 1997, Chanut and Heberlein, 1997, Burke and Basler, 1996, Shen and Mardon, 1997, van den Heuvel and Ingham, 1996, Chen and Baker, 1997, Mullor et al., 1997, Epps et al., 1997, Heslip et al., 1997, Kenyon et al., 2003, Pignoni and Zipursky, 1997, Morimura et al., 1996, Penton et al., 1997, Johnston and Schubiger, 1996, Chanut and Heberlein, 1997, Brook and Cohen, 1996, Jiang and Struhl, 1998, Chen and Struhl, 1996, Sturtevant et al., 1997, Pignoni et al., 1997, Gorfinkiel et al., 2005, Horabin, 2005, Stultz et al., 2006, Dussillol-Godar et al., 2006, Rogers et al., 2005, Kent et al., 2006, Wang and Struhl, 2004, Ekas et al., 2006, Zhao et al., 2007, Kugler and Nagel, 2007, Hennig et al., 2006, Kennell et al., 2008, Legent et al., 2012, Shi et al., 2011, Mukherjee et al., 2012, Malpel et al., 2007, Callejo et al., 2006, Thompson et al., 2005, Miura et al., 2008, Vrailas and Moses, 2006, Lee et al., 2006, Tanaka-Matakatsu et al., 2007, Svendsen et al., 2009, Zhang et al., 2009, Khaliullina et al., 2009, Zhang et al., 2005, Usha and Shashidhara, 2010, Jia et al., 2009, Seong et al., 2010, Zhang et al., 2006, Du et al., 2011, Jia et al., 2010, Fan et al., 2012, Wang and Sun, 2012, Marchal et al., 2012, Zhang et al., 2011, Chang et al., 2008, Zheng et al., 2010, Vyas et al., 2008)
Molecular data	dpp promoter drives Ecol\lacZ expression. (Freeland and Kuhn, 1996) Ecol\lacZ gene expression is controlled by the 3' dpp enhancer. (Buratovich and Bryant, 1995) Ecol\lacZ gene expression is driven by an dpp promoter. (Capdevila et al., 1994) Construct: A dpp disc enhancer drives Ecol\lacZ expression. (Kojima et al., 1994) 10kb EcoRI fragment spanning dpp region disk III--II boundary drives Ecol\lacZ expression. (Heberlein et al., 1993) 12kb EcoRI fragment spanning dpp region disk III--II boundary drives Ecol\lacZ expression. (Blackman et al., 1991) Ecol\lacZ expression is driven by fragment from 106.9-116.9kb of dpp, at the disk III-disk II boundary. (Raftery et al., 1991)
Phenotypic class
Phenotype manifest in
Other information

Allele	ry^+t7.2
Reference(s)	(Blackman et al., 1991)
Molecular data	Construct: 7.2kb HindIII fragment that contains ry⁺ function. (Rubin and Spradling, 1983)
Phenotypic class	wild-type (Rubin and Spradling, 1983)
Phenotype manifest in
Other information
Expression Data
Reporter Expression
No Assay Recorded Stage Tissue/Position (including subcellular localization) Reference embryonic stage 15 procephalic segment (Blackman et al., 1991) first instar larval stage imaginal disc primordium (Blackman et al., 1991) third instar larval stage male genital disc \| restricted (Blackman et al., 1991) eye-antennal disc \| restricted (Khalsa et al., 1998) embryonic/larval brain \| restricted (Blackman et al., 1991) ventral thoracic disc \| restricted (Blackman et al., 1991) distribution deduced from reporter Stage Tissue/Position (including subcellular localization) Reference embryonic stage 15 embryonic brain (Blackman et al., 1991) thoracic segment (Blackman et al., 1991) larval stage morphogenetic furrow (Mukherjee et al., 2000) eye-antennal disc \| restricted (Mukherjee et al., 2000) ventral thoracic disc \| anterior/posterior compartment boundary (Mukherjee et al., 2000) first instar larval stage embryonic/larval labral segment sense organ (Blackman et al., 1991) third instar larval stage eye disc \| restricted (Hazelett et al., 1998) ventral thoracic disc \| restricted (Khalsa et al., 1998) female genital disc \| restricted (Freeland and Kuhn, 1996, Blackman et al., 1991, Gorfinkiel et al., 1999) female repressed primordium (Gorfinkiel et al., 1999) male genital disc \| restricted (Freeland and Kuhn, 1996, Gorfinkiel et al., 1999) wing disc \| restricted (Capdevila and Guerrero, 1994, Blackman et al., 1991, Khalsa et al., 1998) eye-antennal disc \| restricted (Blackman et al., 1991) morphogenetic furrow (Epps et al., 1997) wing disc \| anterior/posterior compartment boundary (Capdevila and Guerrero, 1994)
Additional Information
Statement Reference Expressed in cells on anterior side of the A/P boundary. (Capdevila and Guerrero, 1994) Expression is observed in both the male and female genital discs, and is complementary to that of wg. Expression is excluded from the repressed male primordium in the female genital disc. (Gorfinkiel et al., 1999) The dpp-:-Ecol\lacZreporter construct reflects wildtype expression of dpp in the imaginal discs.Expression is detected in a stripe along the anterior-posterior boundary of thewing disc and along the morphogenetic forrow in the eye-antennal disc. (Khalsa et al., 1998) The expression pattern of Ecol\lacZ^dpp.BS3.0 most closely recapitulates wild-type dpp expression patterns during development. (Blackman et al., 1991)

Marker for	female genital disc (Gorfinkiel et al., 1999) male genital disc (Gorfinkiel et al., 1999)
Reflects expression of	dpp (Hazelett et al., 1998, Freeland and Kuhn, 1996, Khalsa et al., 1998, Blackman et al., 1991, Gorfinkiel et al., 1999, Mukherjee et al., 2000)
Progenitors & Descendants
progenitor(s)	pP{BS3.0} (Blackman et al., 1991)
descendant(s)
Comments
	construct_comment: One of a series of lacZ reporter constructs used to characterize the disk 3\' regulatory region of dpp. (Blackman et al., 1991)
Stocks Listed in FlyBase ( 2 )
Bloomington	5527 P{BS3.0}H1-1, cn¹; ry⁵⁰⁶ 5528 cn¹; P{BS3.0}H1-2, ry⁵⁰⁶/TM2
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	(Dpp)-lacZ line 3.0 (Liu et al., 2008) BS 3.0 (Lai et al., 2000) BS3.0 (Rogers et al., 2005, Svendsen et al., 2009, Zheng et al., 2010) BS3.0 decapentaplegic (Vrailas and Moses, 2006) BS3.0 dpp LacZ (Collier et al., 2004) BS3.0dpp-lacZ (Capdevila and Guerrero, 1994) BS3.0dpp-lacZ BS3.0 (Blackman et al., 1991) dpp57-lacZ (Ghbeish and McKeown, 2002) dpp:lacZ (Rogers et al., 2005, Vrailas and Moses, 2006) dpp^BS3.0-lacz (Usha and Shashidhara, 2010) dppBS3.0 (Bras-Pereira et al., 2006) dppBS3.0:LacZ (Kent et al., 2006) dpp lacZ (Ghazi et al., 2003) dpp-LacZ (Belenkaya et al., 2002, Collier et al., 2004, Shi et al., 2011, Lee et al., 2006) Dpp-lacZ (Erkner et al., 2002, Ogden et al., 2006, Liu et al., 2008, Vyas et al., 2008) dpplacZ (Haines and van Den Heuvel, 2000) dpp-lacZ (Ekas et al., 2006, Horabin, 2005, Wang and Struhl, 2004, Pfleger et al., 2007, Jia et al., 2005, Yao et al., 2006, Zhang et al., 2006, Du et al., 2011, Zhao et al., 2007, Kugler and Nagel, 2007, Malpel et al., 2007, Kennell et al., 2008, Thompson et al., 2005, Shi et al., 2011, Mosimann et al., 2006, Svendsen et al., 2009, Zhang et al., 2005, Usha and Shashidhara, 2010, Jia et al., 2009, Jia et al., 2010, Mukherjee et al., 2012, Zheng et al., 2010, Fan et al., 2012, Wang and Sun, 2012, Zhang et al., 2011, Vyas et al., 2008) dpp-lacZ3.0 (Greenwood and Struhl, 1999) dpp-lacZ(BS3.0) (Jang et al., 2003) dpp-lacZ (BS 3.0) (FlyBase, 1996-, Kennell et al., 2008) dpp-lacZ (BS3.0) (Glise et al., 2005, Tanaka-Matakatsu et al., 2007) dpp-lacZ{BS3.0} (Miura et al., 2008) dpp-LacZ<up>BS 3.0</up> (Torroja et al., 2004) dpp-lacZ^BS3.0 (Gorfinkiel et al., 2005, Stultz et al., 2006, Legent et al., 2012, de Navas et al., 2006, Hennig et al., 2006, Seong et al., 2010) dpp-LacZ^BS3.0 (Callejo et al., 2006, Zhang et al., 2009) dpplacZ^BS3.0 (Khaliullina et al., 2009) dpp-lacZ^dpp.BS3.0 (Marenda et al., 2004) dpp-lacZBS3.0 (Niwa et al., 2004) dpp-lacZ BS3.0 (Kenyon et al., 2003, Cadigan et al., 2002, Niwa et al., 2000, Miletich and Limbourg-Bouchon, 2000, Dussillol-Godar et al., 2006, Marchal et al., 2012) dpp-Z (Pichaud and Casares, 2000) dppZ (Bras-Pereira et al., 2006, Shi et al., 2011, Zheng et al., 2010, Zhang et al., 2011) DppZ (Fan et al., 2012) P[dpp-lacZ] (Lin et al., 2003) P{BS3.0} (FlyBase, 1996-) P{dpp-lacZ} (Mukherjee et al., 2012) P{dpp-lacZ}dpp-lacZ (Diaz-Benjumea et al., 1994) P^dpp-lacZ (Chang et al., 2008)
Secondary FlyBase IDs

References ( 230 )

Generate a list of	All references relating to this transgenic transposon ( 230 )

List References by type	Research paper ( 228 ) Letter ( 2 )
Recent research papers ( 8 )
	Fan et al., 2012, Dev. Biol. 366(2): 172--184 Hh-induced Smoothened conformational switch is mediated by differential phosphorylation at its C-terminal tail in a dose- and position-dependent manner. [FBrf0218374] Legent et al., 2012, Genetics 190(2): 601--616 A screen for x-linked mutations affecting Drosophila photoreceptor differentiation identifies casein kinase 1α as an essential negative regulator of wingless signaling. [FBrf0217484] Marchal et al., 2012, PLoS ONE 7(3): e34310 The HIV-1 Vpu Protein Induces Apoptosis in Drosophila via Activation of JNK Signaling. [FBrf0218002] Mukherjee et al., 2012, G3 (Bethesda) 2(1): 23--28 Genetic analysis of fibroblast growth factor signaling in the Drosophila eye. [FBrf0217664] Wang and Sun, 2012, Development 139(18): 3413--3421 Segregation of eye and antenna fates maintained by mutual antagonism in Drosophila. [FBrf0219201] Du et al., 2011, PLoS ONE 6(9): e24168 In Vivo RNAi Screen Reveals Neddylation Genes as Novel Regulators of Hedgehog Signaling. [FBrf0215574] Shi et al., 2011, Development 138(19): 4219--4231 The Hedgehog-induced Smoothened conformational switch assembles a signaling complex that activates Fused by promoting its dimerization and phosphorylation. [FBrf0216910] Zhang et al., 2011, Cell Res. 21(10): 1436--1451 Transduction of the Hedgehog signal through the dimerization of Fused and the nuclear translocation of Cubitus interruptus. [FBrf0217260] Show all research papers ...