Recombinant construct P{UAS-Rac1.L89}

General Information
Symbol	P{UAS-Rac1.L89}	FlyBase ID	FBtp0000748
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	1 available
Molecular map
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Luo et al., 1994, FlyBase, 1992-)
Uses	CV term Qualifiers & info Reference binary system (targeted) Rac1 (Luo et al., 1994) Rac1 \| modified gene (Luo et al., 1994) characterization Rac1 \| protein (Luo et al., 1994)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference binary component (Luo et al., 1994) chimeric gene (Luo et al., 1994) modified gene (Luo et al., 1994) selectable marker (Luo et al., 1994)
Component Alleles
Allele	Rac1^L89.Scer\UAS
Reference(s)	(Medioni and Noselli, 2005, Kaufmann et al., 1998, Sepp and Auld, 2003, Prokopenko et al., 1999, Magie et al., 2002, Luo et al., 1994, Paululat et al., 1999, Lee and Luo, 1999, Long et al., 2006, Lovegrove et al., 2006, Pirraglia et al., 2006, Paladi and Tepass, 2004, Beckett et al., 2008, Assaker et al., 2010)
Molecular data	Construct: Amino acid replacement: S89L. A dominant-negative form of Rac1 is expressed under the control of Scer\UAS regulatory sequences. (Luo et al., 1994)
Phenotypic class	lethal \| first instar larval stage (Luo et al., 1994) lethal \| first instar larval stage, with Scer\GAL4⁶⁰ (Luo et al., 1994) lethal \| first instar larval stage, with Scer\GAL4^how-24B (Luo et al., 1994) neuroanatomy defective, with Scer\GAL4^GMR.PF (Long et al., 2006) visible, with Scer\GAL4⁵⁴ (Lee and Luo, 1999)
Phenotype manifest in	abdominal intersegmental nerve (Luo et al., 1994) abdominal intersegmental nerve, with Scer\GAL4^elav-C155 (Kaufmann et al., 1998) axon, with Scer\GAL4^elav-C155 (Kaufmann et al., 1998) border follicle cell, with Scer\GAL4^slbo.2.6 (Medioni and Noselli, 2005, Assaker et al., 2010) embryonic myoblast, with Scer\GAL4^Mef2.5xCD (Beckett et al., 2008) eye, with Scer\GAL4⁵⁴ (Lee and Luo, 1999) macrophage, with Scer\GAL4^{Cg25C-A109.1F2.P} (Paladi and Tepass, 2004) macrophage, with Scer\GAL4^gcm-rA87.P (Paladi and Tepass, 2004) myoblast, with Scer\GAL4^how-24B (Luo et al., 1994) peripheral glial cell, with Scer\GAL4^repo (Sepp and Auld, 2003) photoreceptor cell & axon, with Scer\GAL4^GMR.PF (Long et al., 2006) polar follicle cell, with Scer\GAL4^slbo.2.6 (Medioni and Noselli, 2005) presumptive embryonic salivary gland, with Scer\GAL4^fkh.PH (Pirraglia et al., 2006) primordium of embryonic/larval spiracle, with Scer\GAL4^ems.HRE (Lovegrove et al., 2006)
Other information

Allele	w^+mC
Reference(s)	(Luo et al., 1994)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	pP{UAS-Rac1.L89} (FlyBase, 1992-)
descendant(s)
Comments
	construct_comment: One of a series of UAS constructs with single amino acid modifications in the Rac1 coding region. (Luo et al., 1994)
Stocks Listed in FlyBase ( 4 )
Bloomington	6290 w^; P{UAS-Rac1.L89}6 5129 w^; P{GawB}LL54, P{UAS-Rac1.L89}6/CyO
Kyoto	108723 y¹ w^; P{UAS-Rac1.L89}6 108060 w^; P{GawB}LL54, P{UAS-Rac1.L89}6/CyO
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{UAS-Drac1.L89} (FlyBase, 1996-) P{UAS-Rac1.L89} (FlyBase, 1996-) P{w⁺mC=UAS-Rac1.L89} (Paladi and Tepass, 2004) UAS-Drac1^L89.6 (Lee and Luo, 1999) UAS-Drac1L89 (Luo et al., 1994) UAS-DracIL89 (Sepp and Auld, 2003) UAS-Rac1^L89 (Pirraglia et al., 2006, Paladi and Tepass, 2004) UAS-Rac^L89.6 (Beckett et al., 2008) UAS-Rac^L89 (Magie et al., 2002, Prokopenko et al., 1999) UAS-RacL89 (Long et al., 2006, Lovegrove et al., 2006) UAS-racL89.6 (Assaker et al., 2010)
Secondary FlyBase IDs

References ( 15 )
Research paper	Assaker et al., 2010, Proc. Natl. Acad. Sci. U.S.A. 107(52): 22558--22563 Spatial restriction of receptor tyrosine kinase activity through a polarized endocytic cycle controls border cell migration. [FBrf0212585] Beckett et al., 2008, Gene Expr. Patterns 8(2): 87--91 Expression and functional analysis of a novel Fusion Competent Myoblast specific GAL4 driver. [FBrf0202959] Long et al., 2006, Genetics 172(3): 1633--1642 De novo GMP synthesis is required for axon guidance in Drosophila. [FBrf0190740] Lovegrove et al., 2006, Curr. Biol. 16(22): 2206--2216 Coordinated control of cell adhesion, polarity, and cytoskeleton underlies Hox-induced organogenesis in Drosophila. [FBrf0194228] Pirraglia et al., 2006, Dev. Biol. 290(2): 435--446 Rac function in epithelial tube morphogenesis. [FBrf0190152] Medioni and Noselli, 2005, Development 132(13): 3069--3077 Dynamics of the basement membrane in invasive epithelial clusters in Drosophila. [FBrf0187473] Paladi and Tepass, 2004, J. Cell Sci. 117(26): 6313--6326 Function of Rho GTPases in embryonic blood cell migration in Drosophila. [FBrf0187835] Sepp and Auld, 2003, Development 130(9): 1825--1835 RhoA and Rac1 GTPases mediate the dynamic rearrangement of actin in peripheral glia. [FBrf0158880] Magie et al., 2002, Development 129(16): 3771--3782 Rho1 interacts with p120(ctn) and alpha-catenin, and regulates cadherin-based adherens junction components in Drosophila. [FBrf0151270] Lee and Luo, 1999, Neuron 22(3): 451--461 Mosaic analysis with a repressible neurotechnique cell marker for studies of gene function in neuronal morphogenesis. [FBrf0107846] Prokopenko et al., 1999, Genes Dev. 13(17): 2301--2314 A putative exchange factor for rho1 GTPase is required for initiation of cytokinesis in Drosophila. [FBrf0111464] Kaufmann et al., 1998, Development 125(3): 453--461 Drosophila Rac1 controls motor axon guidance. [FBrf0100716] Luo et al., 1994, Genes Dev. 8(15): 1787--1802 Distinct morphogenetic functions of similar small GTPases: Drosophila Drac1 is involved in axonal outgrowth and myoblast fusion. [FBrf0076490]
Review	Paululat et al., 1999, Mech. Dev. 83(1,2): 17--26 Essential genes for myoblast fusion in Drosophila embryogenesis. [FBrf0108990]
FlyBase analysis	FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]