Recombinant construct P{hs-CycA}

General Information
Symbol	P{hs-CycA}	FlyBase ID	FBtp0000958
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	0 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Knoblich and Lehner, 1993)
Uses	CV term Qualifiers & info Reference characterization CycA (Knoblich and Lehner, 1993) heat-shock construct CycA (Knoblich and Lehner, 1993)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference chimeric gene (Knoblich and Lehner, 1993) general promoter (Knoblich and Lehner, 1993) inducible promoter (Knoblich and Lehner, 1993) selectable marker (Knoblich and Lehner, 1993, Lehner et al., 1991, Sigrist et al., 1995)
Component Alleles
Allele	CycA^hs.PK
Reference(s)	(Foley et al., 1999, Lee et al., 2001, Knoblich and Lehner, 1993, Lilly et al., 2000, Ohlmeyer and Schupbach, 2003, Su et al., 2000, Su et al., 1999, Lehner et al., 1991, Sauer et al., 1995, Sigrist et al., 1995, Stratmann and Lehner, 1996, Sprenger et al., 1997, Thomas et al., 1997, Dong et al., 1997, Lilly and Spradling, 1996, Su et al., 1998)
Molecular data	CycA is expressed under the control of a heat shock promoter. (Sprenger et al., 1997) Construct: Expression of CycA cDNA driven from a heat shock promoter. (Knoblich and Lehner, 1993) NcoI-NheI fragment of CycA cDNA is driven from an Hsp70 promoter. (Lehner et al., 1991)
Phenotypic class	mitotic cell cycle defective (Dong et al., 1997, Ohlmeyer and Schupbach, 2003, Lehner et al., 1991) wild-type (Knoblich and Lehner, 1993)
Phenotype manifest in	amnioserosa (Lehner et al., 1991) egg chamber (Ohlmeyer and Schupbach, 2003) egg chamber & cell (Lilly et al., 2000) egg chamber & mitotic cell cycle (Lilly et al., 2000) eye (Dong et al., 1997) mitotic cell cycle (Sprenger et al., 1997) mitotic cell cycle & epidermis & embryo (Sprenger et al., 1997) pole cell (Su et al., 1998) S phase \| ectopic (Thomas et al., 1997)
Other information

Allele	w^+mC
Reference(s)	(Knoblich and Lehner, 1993, Lehner et al., 1991, Sigrist et al., 1995)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	pP{hs-CycA} (Knoblich and Lehner, 1993)
descendant(s)
Comments
	construct_comment: Used to investigate the synergistic actionof CycA and CycB proteins during the cell cycle. (Knoblich and Lehner, 1993)
Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	hs-cycA (Lee et al., 2001, Foley et al., 1999, Su et al., 1999, Knoblich and Lehner, 1993) HS-cyclin A (Ohlmeyer and Schupbach, 2003) Hs-cyclin A (Lilly et al., 2000) P{hs-CycA} (FlyBase, 1996-)
Secondary FlyBase IDs

References ( 16 )
Research paper	Ohlmeyer and Schupbach, 2003, Development 130(25): 6339--6349 Encore facilitates SCF-Ubiquitin-proteasome-dependent proteolysis during Drosophila oogenesis. [FBrf0167518] Lee et al., 2001, Genetics 158(4): 1545--1556 A genetic screen for suppressors and enhancers of the Drosophila pan gu cell cycle kinase identifies cyclin B as a target. [FBrf0138402] Lilly et al., 2000, Dev. Biol. 218(1): 53--63 Cyclin A associates with the fusome during germline cyst formation in the Drosophila ovary. [FBrf0125379] Su et al., 2000, Curr. Biol. 10(3): 119--126 Activating the DNA damage checkpoint in a developmental context. [FBrf0125156] Foley et al., 1999, Curr. Biol. 9(23): 1392--1402 Rux is a cyclin-dependent kinase inhibitor (CKI) specific for mitotic cyclin-Cdk complexes. [FBrf0123013] Su et al., 1999, Curr. Biol. 9(16): 919--922 Drosophila grapes/CHK1 mutants are defective in cyclin proteolysis and coordination of mitotic events. [FBrf0111496] Su et al., 1998, Dev. Biol. 196(2): 160--170 The cell cycle program in germ cells of the Drosophila embryo. [FBrf0102404] Dong et al., 1997, Genes Dev. 11(1): 94--105 Control of G1 in the developing Drosophila eye: rca1 regulates Cyclin A. [FBrf0091048] Sprenger et al., 1997, Curr. Biol. 7(7): 488--499 S-phase function of Drosophila cyclin A and its downregulation in G1 phase. [FBrf0096324] Thomas et al., 1997, Genes Dev. 11(10): 1289--1298 roughex down-regulates G2 cyclins in G1. [FBrf0093752] Lilly and Spradling, 1996, Genes Dev. 10(19): 2514--2526 The Drosophila endocycle is controlled by Cyclin E and lacks a checkpoint ensuring S-phase completion. [FBrf0090669] Stratmann and Lehner, 1996, Cell 84(1): 25--35 Separation of sister chromatids in mitosis requires the Drosophila pimples product, a protein degraded after the metaphase/anaphase transition. [FBrf0085992] Sauer et al., 1995, Genes Dev. 9(11): 1327--1339 Distinct modes of cyclin E/cdc2c kinase regulation and S-phase control in mitotic and endoreduplication cycles of Drosophila embryogenesis. [FBrf0082560] Sigrist et al., 1995, EMBO J. 14(19): 4827--4838 Exit from mitosis is regulated by Drosophila fizzy and the sequential destruction of cyclins A, B and B3. [FBrf0084373] Knoblich and Lehner, 1993, EMBO J. 12(1): 65--74 Synergistic action of Drosophila cyclins A and B during the G₂-M transition. [FBrf0058164] Lehner et al., 1991, Cold Spring Harbor Symp. Quant. Biol. 56: 465--475 Exploring the role of Drosophila cyclin A in the regulation of S phase. [FBrf0053446]