Recombinant construct P{f⁺13}

General Information
Symbol	P{f⁺13}	FlyBase ID	FBtp0000960
Feature type	transgenic_transposon

Size	18.285	Expression data
Associated insertions	8 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Petersen et al., 1994)
Uses	CV term Qualifiers & info Reference characterization f (Petersen et al., 1994)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)	FlyBase-compiled
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size	18.285
Left end	P5\' (Petersen et al., 1994)
Right end	P3\' (Petersen et al., 1994)

Segments	Number Orientation Symbol 1 + CaSpeR_P5'(P5',Sp) 2 + 001181 3 + f⁺13.0(r) 4 + 001182 5 + Car1_P3'(H3,P3')
Reference	(Petersen et al., 1994)
Features
	CV term Qualifiers & info Reference complete rescue (Zecca et al., 1995) partial rescue (Pirrotta, 1988) selectable marker (Zecca et al., 1995, Diaz-Benjumea and Cohen, 1993, Petersen et al., 1994, Diaz-Benjumea and Hafen, 1994, de Celis et al., 1995, Gomez-Skarmeta et al., 1996, Burke and Basler, 1996, de Celis et al., 1996, Boedigheimer et al., 1997, Tilney et al., 1998) selectable marker (Pirrotta, 1988)
Component Alleles
Allele	f^+t13
Reference(s)	(Zecca et al., 1995, Tilney et al., 2000, Diaz-Benjumea and Cohen, 1993, Tilney et al., 2003, Petersen et al., 1994, Diaz-Benjumea and Hafen, 1994, Tilney et al., 2004, Tilney et al., 1998, Burke and Basler, 1996, de Celis et al., 1995, Gomez-Skarmeta et al., 1996, de Celis et al., 1996, Boedigheimer et al., 1997, Duboff et al., 2012)
Molecular data	Construct: 13kb NotI-SalI fragment from the genomic cosmid clone CoSpeR-forked-12-2, including the coding regions for the 2.5kb, 1.9kb and 1.1kb f transcripts. (Petersen et al., 1994)
Phenotypic class	visible (Tilney et al., 2000, Tilney et al., 1998) wild-type (Petersen et al., 1994)
Phenotype manifest in	adult brain (Duboff et al., 2012) macrochaeta (Tilney et al., 1998) macrochaeta & actin filament (Tilney et al., 1998) microchaeta (Tilney et al., 1998, Tilney et al., 2004) microchaeta & actin filament (Tilney et al., 2000, Tilney et al., 2003) mitochondrion (Duboff et al., 2012)
Other information

Allele	w^+mC
Reference(s)	(Pirrotta, 1988, Petersen et al., 1994)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	pP{f⁺13} (Petersen et al., 1994)
descendant(s)
Comments

Stocks Listed in FlyBase ( 16 )
Bloomington	2191 f^36a; P{f⁺13}44C/CyO 2228 f^36a; P{f⁺13}87D/TM3, Sb¹ 2180 f^36a; P{f⁺13}l(2)37Co¹/CyO 2230 f^36a; mwh¹ jv¹ P{f⁺13}77A/TM2 42722 w^* f^36a; P{f⁺13}30B ck¹³ P{neoFRT}40A/CyO 2218 y¹ f^36a; P{f⁺13}77A/TM3, Sb¹ Ser¹ 2202 f^36a; P{f⁺13}30B ck¹³ pr¹ pwn¹/CyO 2206 f^36a; pr¹ pwn¹ P{f⁺13}44C P{f⁺13}47B/CyO 4364 f^36a; pr¹ pwn¹/CyO; M(3)65F¹ P{f⁺13}77A/TM2
Kyoto	101864 y¹ f^36a ; P{f⁺13}30B / CyO 101904 f^36a ; mwh¹ jv¹ P{f⁺13}77A / TM2 101814 f^36a ; P{f⁺13}44C P{f⁺13}52 M(2)58F¹ / CyO
	More stocks available...
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	forked⁺ (Duboff et al., 2012) forked^+t13 (Duboff et al., 2012) P[f⁺] (de Celis et al., 1995) P[w⁺,f⁺] (Petersen et al., 1994) P{f⁺13} (FlyBase, 1996-)
Secondary FlyBase IDs

References ( 13 )
Research paper	Duboff et al., 2012, Neuron 75(4): 618--632 Tau Promotes Neurodegeneration via DRP1 Mislocalization In Vivo. [FBrf0219271] Tilney et al., 2004, Mol. Biol. Cell 15(12): 5481--5491 The role actin filaments play in providing the characteristic curved form of Drosophila bristles. [FBrf0180507] Tilney et al., 2003, Mol. Biol. Cell 14(10): 3953--3966 Actin filament turnover regulated by cross-linking accounts for the size, shape, location, and number of actin bundles in Drosophila bristles. [FBrf0167860] Tilney et al., 2000, J. Cell Biol. 148(1): 87--99 Regulation of actin filament cross-linking and bundle shape in Drosophila bristles. [FBrf0123218] Tilney et al., 1998, J. Cell Biol. 143(1): 121--133 Why are two different cross-linkers necessary for actin bundle formation in vivo and what does each cross-link contribute? [FBrf0105481] Boedigheimer et al., 1997, Dev. Genet. 20(2): 103--110 Expanded functions in the apical cell domain to regulate the growth rate of imaginal discs. [FBrf0093371] Burke and Basler, 1996, Development 122(7): 2261--2269 Dpp receptors are autonomously required for cell proliferation in the entire developing Drosophila wing. [FBrf0088035] de Celis et al., 1996, Development 122(9): 2719--2728 Functional relationships between N, Su(H) and the bHLH genes of the E(spl) complex: the E(spl) genes mediate only a subset of Notch activities during imaginal development. [FBrf0090023] Gomez-Skarmeta et al., 1996, Cell 85(1): 95--105 Araucan and caupolican, two members of the novel Iroquois complex, encode homeoproteins that control proneural and vein-forming genes. [FBrf0086419] de Celis et al., 1995, Development 121(10): 3405--3416 ventral veinless, the gene encoding the Cf1a transcription factor, links positional information and cell differentiation during embryonic and imaginal development in Drosophila melanogaster. [FBrf0084541] Diaz-Benjumea and Hafen, 1994, Development 120(3): 569--578 The sevenless signalling cassette mediates Drosophila EGF receptor function during epidermal development. [FBrf0072959] Petersen et al., 1994, Genetics 136(1): 173--182 forked proteins are components of fiber bundles present in developing bristles of Drosophila melanogaster. [FBrf0068634] Diaz-Benjumea and Cohen, 1993, Cell 75(4): 741--752 Interaction between dorsal and ventral cells in the imaginal disc directs wing development in Drosophila. [FBrf0064428]

Recombinant construct P{f+13}

Recombinant construct P{f⁺13}