Recombinant construct P{Act5C(-FRT)wg.S}

General Information
Symbol	P{Act5C(-FRT)wg.S}	FlyBase ID	FBtp0001060
Feature type	transgenic_transposon

Size	14.624	Expression data
Associated insertions	0 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Struhl and Basler, 1993)
Uses	CV term Qualifiers & info Reference characterization wg \| mosaic analysis (Struhl and Basler, 1993) ubiquitous expression wg (Struhl and Basler, 1993)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)	FlyBase-compiled
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size	14.624
Left end	P5\' (Struhl and Basler, 1993)
Right end	P3\' (Struhl and Basler, 1993)

Segments	Number Orientation Symbol 1 + Car1_P5'(P5',R1) 2 + 001209 3 - wg-αTub 4 - 001212 5 - 001216 6 - <FRT(J3) 7 - 001217 8 - 001211 9 - Act5C⁺4.4(R1,ATG) 10 + 001210 11 + Car20_P3'(H3,P3')
Reference	(Struhl and Basler, 1993)
Features
	CV term Qualifiers & info Reference chimeric gene (Struhl and Basler, 1993) complete rescue (Spradling and Rubin, 1983) general promoter (Struhl and Basler, 1993) selectable marker (Rubin and Spradling, 1983, Struhl and Basler, 1993)
Component Alleles
Allele	ry^+t7.2
Reference(s)	(Rubin and Spradling, 1983, Struhl and Basler, 1993)
Molecular data	Construct: 7.2kb HindIII fragment that contains ry⁺ function. (Rubin and Spradling, 1983)
Phenotypic class	wild-type (Rubin and Spradling, 1983)
Phenotype manifest in
Other information

Allele	wg^Act5C.PS
Reference(s)	(Struhl and Basler, 1993, Heberlein et al., 1998, Maves and Schubiger, 1998, Maves and Schubiger, 1995, Joulia et al., 2005, Tomlinson et al., 1997, Sustar and Schubiger, 2005, Sanchez et al., 1997, Emerald and Roy, 1998, Royet and Finkelstein, 1996, Tomlinson, 2003, Lecuit and Cohen, 1997, Brook and Cohen, 1996, McClure and Schubiger, 2008, McClure et al., 2008, Schubiger et al., 2010)
Molecular data	wg expression driven by the Act5C promoter. (Treisman and Rubin, 1995) Scer\FRT-y^+t7.7 cassette has been excised, juxtaposing Act5C promoter with wg coding sequences. (Struhl and Basler, 1993)
Phenotypic class	lethal \| pharate adult stage \| somatic clone (Joulia et al., 2005) visible (Sustar and Schubiger, 2005, Tomlinson, 2003, Emerald and Roy, 1998) visible \| homeotic \| somatic clone (Joulia et al., 2005) visible \| somatic clone (McClure and Schubiger, 2008)
Phenotype manifest in	anal plate (Sanchez et al., 1997) dorsal margin photoreceptor \| ectopic \| somatic clone (Tomlinson, 2003) female gonopod \| dorsal (Sanchez et al., 1997) female gonopod \| ventral (Sanchez et al., 1997) frons (Royet and Finkelstein, 1996) gonopod long bristle (Emerald and Roy, 1998) gonopod thorn bristle (Emerald and Roy, 1998) interommatidial bristle (Treisman and Rubin, 1995) labellum \| somatic clone (Joulia et al., 2005) lateral ocellus (Royet and Finkelstein, 1996) leg (Diaz-Benjumea and Cohen, 1994) leg & cuticle \| dorsal (Maves and Schubiger, 1995) leg \| somatic clone (McClure and Schubiger, 2008) male genitalia (Emerald and Roy, 1998) male terminalia sensillum (Emerald and Roy, 1998) ommatidium (Treisman and Rubin, 1995, Tomlinson et al., 1997) penis (Sanchez et al., 1997, Emerald and Roy, 1998) pigment cell \| ectopic \| somatic clone (Tomlinson, 2003) prothoracic leg (Maves and Schubiger, 1998) pseudotracheal sensillum \| somatic clone \| supernumerary (Joulia et al., 2005) retina \| somatic clone (Tomlinson, 2003) sex comb \| ectopic \| somatic clone (Joulia et al., 2005) ventral thoracic disc (Sustar and Schubiger, 2005) wing \| ectopic \| somatic clone (McClure and Schubiger, 2008) wing hinge \| ectopic (Maves and Schubiger, 1995, Sustar and Schubiger, 2005) yellow club \| ectopic (Sustar and Schubiger, 2005)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	P{Act5C(FRT.y⁺)wg.S} (Struhl and Basler, 1993, Basler and Struhl, 1994)
descendant(s)
Comments
	construct_comment: Contains a single FRT site. (Struhl and Basler, 1993)
Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	Act5C>wg (Struhl and Basler, 1993, McClure and Schubiger, 2008) Act>wg (McClure et al., 2008) actin-wg (Tomlinson, 2003) P{Act5C(-FRT)wg.S} (FlyBase, 1996-) P{Act5C>wg} (FlyBase, 1996-) P{Act5C-wg.S} (FlyBase, 1996-) P{wg.Act5C.S} (FlyBase, 1996-)
Secondary FlyBase IDs

References ( 17 )
Research paper	Schubiger et al., 2010, Dev. Biol. 347(2): 315--324 Regeneration and transdetermination: The role of wingless and its regulation. [FBrf0212048] McClure and Schubiger, 2008, Mech. Dev. 125(1-2): 67--80 A screen for genes that function in leg disc regeneration in Drosophila melanogaster. [FBrf0203146] McClure et al., 2008, Dev. Biol. 319(1): 68--77 Three genes control the timing, the site and the size of blastema formation in Drosophila. [FBrf0205073] Joulia et al., 2005, Dev. Biol. 278(2): 496--510 Homeotic proboscipedia function modulates hedgehog-mediated organizer activity to pattern adult Drosophila mouthparts. [FBrf0183854] Sustar and Schubiger, 2005, Cell 120(3): 383--393 A transient cell cycle shift in Drosophila imaginal disc cells precedes multipotency. [FBrf0183774] Tomlinson, 2003, Dev. Cell 5(5): 799--809 Patterning the peripheral retina of the fly: decoding a gradient. [FBrf0167480] Emerald and Roy, 1998, Dev. Genes Evol. 208(9): 504--516 Organising activities of engrailed, hedgehog, wingless and decapentaplegic in the genital discs of Drosophila melanogaster. [FBrf0105224] Heberlein et al., 1998, Development 125(4): 567--577 Dorsoventral patterning in the Drosophila retina by wingless. [FBrf0100748] Maves and Schubiger, 1998, Development 125(1): 115--124 A molecular basis for transdetermination in Drosophila imaginal discs: interactions between wingless and decapentaplegic signaling. [FBrf0100156] Lecuit and Cohen, 1997, Nature 388(6638): 139--145 Proximal-distal axis formation in the Drosophila leg. [FBrf0095553] Sanchez et al., 1997, Dev. Genes Evol. 207(4): 229--241 The genital disc of Drosophila melanogaster. [FBrf0098968] Tomlinson et al., 1997, Development 124(22): 4515--4521 Linking Frizzled and Wnt signaling in Drosophila development. [FBrf0099729] Brook and Cohen, 1996, Science 273(5280): 1373--1377 Antagonistic interactions between wingless and decapentaplegic responsible for dorsal-ventral pattern in the Drosophila Leg. [FBrf0089604] Royet and Finkelstein, 1996, Development 122(6): 1849--1858 hedgehog, wingless and orthodenticle specify adult head development in Drosophila. [FBrf0088886] Maves and Schubiger, 1995, Development 121(5): 1263--1272 wingless induces transdetermination in developing Drosophila imaginal discs. [FBrf0078989] Basler and Struhl, 1994, Nature 368(6468): 208--214 Compartment boundaries and the control of Drosophila limb pattern by hedgehog protein. [FBrf0068409] Struhl and Basler, 1993, Cell 72(4): 527--540 Organizing activity of wingless protein in Drosophila. [FBrf0057889]