Recombinant construct P{pUCPlac-1}

General Information
Symbol	P{pUCPlac-1}	FlyBase ID	FBtp0001075
Feature type	transgenic_transposon

Size	7.883	Expression data
Associated insertions	0 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Molsberger et al., 1988)
Uses	CV term Qualifiers & info Reference carrier transposon P-element (Molsberger et al., 1988) reporter (Molsberger et al., 1988)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)	FlyBase-compiled
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size	7.883
Left end	P5\' (Molsberger et al., 1988)
Right end	P3\' (Molsberger et al., 1988)

Segments	Number Orientation Symbol 1 + 6.1_P5'(P5',Xm) 2 + 001036 3 + pUC8(Nr1,Pv2) 4 + pUCPlac.mcs1 5 + lacZ⁺3.06(-P).2 6 + 001244 7 + pUC8(Pv2,Nr1) 8 + 001037 9 + Hsp70::neo_1.4(Xb,Sm) 10 + 001039 11 + 6.1_P3'(X1,P3')
Reference	(Molsberger et al., 1988)
Features
	CV term Qualifiers & info Reference bacterial selection (Molsberger et al., 1988) chimeric gene (Steller and Pirrotta, 1985) general promoter (Thummel, 1996.8.8) general promoter (Steller and Pirrotta, 1985) inducible promoter (Thummel, 1996.8.8) inducible promoter (Steller and Pirrotta, 1985) reporter cassette (Molsberger et al., 1988) reporter gene (Molsberger et al., 1988) selectable marker (Steller and Pirrotta, 1985, Molsberger et al., 1988)
Component Alleles
Allele	Ecol\lacZ^pUCPlac
Reference(s)	(Molsberger et al., 1988)
Molecular data	Construct: 3.0kb BamHI fragment of Ecol\lacZ containing coding sequences starting with the 9th amino acid (promoter and operator sequences are deleted). (Molsberger et al., 1988)
Phenotypic class
Phenotype manifest in
Other information

Allele	Tn\neoR^Hsp70Bb.PS
Reference(s)	(Steller and Pirrotta, 1985, Molsberger et al., 1988)
Molecular data	Construct: Tn\neoR gene lacking the bacterial promoter but driven by 465bp Xba Xmn fragment of the Hsp70 heat shock promoter and 206 nucleotides of untranslated leader sequence. (Steller and Pirrotta, 1985)
Phenotypic class
Phenotype manifest in
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	pP{pUCPlac-1} (Molsberger et al., 1988)
descendant(s)
Comments
	construct_comment: P{pUCPlac-2} and P{pUCPlac-3} are equivalent to P{pUCPlac-1} with additional nucleotides flanking the polylinker, allowing insertion into different open reading frames. (Molsberger et al., 1988)
Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{lacZ^pUCPlac ori ampR neoR^Hsp70Bb.PS=pUCPlac-1} (FlyBase, 1996-) P{pUCPlac-1} (FlyBase, 1996-)
Secondary FlyBase IDs

References ( 1 )
Research paper	Molsberger et al., 1988, Gene 63: 147--151 A new set of lacZ fusion vectors, pUCPlac, for studying gene expression in Drosophila by P-mediated transformation. [FBrf0048073]