Recombinant construct P{RMC4}

General Information
Symbol	P{RMC4}	FlyBase ID	FBtp0001089
Feature type	transgenic_transposon

Size	6.453	Expression data
Associated insertions	2 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Bellen et al., 1992)
Uses	CV term Qualifiers & info Reference analytical tool cell ablation (Bellen et al., 1992) carrier transposon cell ablation (Bellen et al., 1992) cell ablation \| (Bellen et al., 1992)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)	FlyBase-compiled
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size	6.453
Left end	P5\' (Bellen et al., 1992)
Right end	P3\' (Bellen et al., 1992)

Segments	Number Orientation Symbol 1 + CaSpeR_P5'(P5',Sp) 2 + 001247 3 + ninaE::DT-A^tsM 4 + 001249 5 + Car1_P3'(H3,P3')
Reference	(Bellen et al., 1992)
Features
	CV term Qualifiers & info Reference cell lethal (Bellen et al., 1992) cell lethal (Bellen et al., 1992) chimeric gene (Bellen et al., 1992) partial rescue (Pirrotta, 1988) selectable marker (Pirrotta, 1988, Bellen et al., 1992)
Component Alleles
Allele	Cbβ\DT-A^tsM.ninaE
Reference(s)	(Sentry et al., 1993, Bellen et al., 1992)
Molecular data	Construct: Amino acid replacement: G53E (GGG to GAG). Mutant variant of Cbβ\DT-A is expressed from the ninaE promoter. (Bellen et al., 1992)
Phenotypic class	visible \| cold sensitive (Bellen et al., 1992)
Phenotype manifest in	ommatidium \| cold sensitive (Bellen et al., 1992)
Other information

Allele	w^+mC
Reference(s)	(Pirrotta, 1988, Bellen et al., 1992)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	pP{RMC4} (Bellen et al., 1992)
descendant(s)
Comments
	mapping_comment: CAUTION: Extent of SV40 sequences may be incorrect. (Bellen et al., 1992) construct_comment: DT-A^tsM is a cold-sensitive mutation of the diphtheria toxin A subunit (DT-A; toxin gene carried by the corynebacteriophage of C. diphtheriae).Selectivity imposed both by temperature sensitivity and by substitution of different promoter sequences. (Bellen et al., 1992) construct_comment: One of a series of DT-A cell ablation vectors containing the ninaE promoter.The ninaE promoter can be excised and replaced using the flanking EcoRI and KpnI sites. (Bellen et al., 1992) sequence comment: CAUTION: Extent of SV40 sequences may be incorrect. (Bellen et al., 1992)
Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{RMC4} (FlyBase, 1996-) P^RMC4 (Bellen et al., 1992)
Secondary FlyBase IDs

References ( 2 )
Research paper	Bellen et al., 1992, Development 114(3): 787--796 Isolation of temperature-sensitive diphtheria toxins in yeast and their effects on Drosophila cells. [FBrf0055894]
Review	Sentry et al., 1993, BioEssays 15(7): 491--493 Conditional cell ablation in Drosophila. [FBrf0057849]