Recombinant construct P1(pAd10-sacBII)

General Information
Symbol	P1(pAd10-sacBII)	FlyBase ID	FBtp0001096
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	0 available
Molecular map
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference P1 vector
Uses	CV term Qualifiers & info Reference cloning vector phage P1 (Hartl et al., 1994)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference bacterial selection (Hartl et al., 1994)
Component Alleles

Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	sacBII_cassette (Hartl et al., 1994)
descendant(s)
Comments
	report comment: Insertion of sacBII_cassette between the BamHI and SalI sites in the tetR gene of P1(pNS582-tet14_Ad10). (Hartl et al., 1994) construct_comment: One of two vectors used by Berkeley Drosophila Genome Project.Reference: Pierce et al., 1992, Proc. Natl. Acad. Sci. USA 89:2056-2060.Insertion in BamHI cloning site prevents expression of sacBII, allowing selection in medium containing high levels of sucrose.PLEASE NOTE: Sequence available for pSacBII, which appears to be the same vector without the Ad10 stuffer fragment (see Descendants). (Hartl et al., 1994)
Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	P1(pAd10-sacBII) (FlyBase, 1996-) pAd10sacBII (Pierce and Sternberg, 1992) pAd10-sacBII (FlyBase, 1996-)
Secondary FlyBase IDs

References ( 2 )
Research paper	Hartl et al., 1994, Proc. Natl. Acad. Sci. U.S.A. 91(15): 6824--6829 Genome structure and evolution in Drosophila: applications of the framework P1 map. [FBrf0073296] Pierce and Sternberg, 1992, Methods Enzymol. 216: 549--574 Using bacteriophage P1 system to clone high molecular weight genomic DNA. [FBrf0057153]