Recombinant construct P{Act5C(-FRT)lacZ.nls1}

General Information
Symbol	P{Act5C(-FRT)lacZ.nls1}	FlyBase ID	FBtp0001102
Feature type	transgenic_transposon

Size		Expression data	lacZ reporter
Associated insertions	0 available
Molecular map
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Struhl and Basler, 1993)
Uses	CV term Qualifiers & info Reference analytical tool mosaic analysis (Struhl and Basler, 1993)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference cell marker (Struhl and Basler, 1993) chimeric gene (Struhl and Basler, 1993) general promoter (Struhl and Basler, 1993) molecular tag (Struhl and Basler, 1993) reporter gene (Struhl and Basler, 1993) selectable marker (Struhl and Basler, 1993)
Component Alleles
Allele	Ecol\lacZ^{Act5C.PS.T:Zzzz\nls1}
Reference(s)	(Struhl and Basler, 1993, Baena-Lopez et al., 2005, Jung et al., 2005, Baena-Lopez et al., 2005, Moreno and Morata, 1999, Rozowski and Akam, 2002, Rangarajan et al., 1999, Emerald and Cohen, 2004, Morrison and Halder, 2010, de la Cova et al., 2004, Tomoyasu et al., 2000, Weigmann and Cohen, 1999, Sun and Tower, 1999, Vegh and Basler, 2003, Perez and Steller, 1996, Reddy et al., 1997, Woodhouse et al., 1998, Lee et al., 2006, Hasegawa et al., 2011)
Molecular data	The Scer\FRT cassette containing a termination codon has been excised, allowing Ecol\lacZ expression from the Actin promoter. (Okabe and Okano, 1997) Scer\FRT cassette has been excised, allowing Ecol\lacZ expression from the Act5C promoter. (Reddy et al., 1997) Fusion of the constitutive Act5C promoter to the Ecol\lacZ coding region. (Brewster and Bodmer, 1995) Scer\FRT-phl^C20 cassette has been excised, juxtaposing Act5C promoter with Ecol\lacZ coding squences. (Struhl and Basler, 1993)
Phenotypic class
Phenotype manifest in
Other information

Allele	ry^+t7.2
Reference(s)	(Struhl and Basler, 1993)
Molecular data	Construct: 7.2kb HindIII fragment that contains ry⁺ function. (Rubin and Spradling, 1983)
Phenotypic class	wild-type (Rubin and Spradling, 1983)
Phenotype manifest in
Other information
Expression Data
Reporter Expression
distribution deduced from reporter Stage Tissue/Position (including subcellular localization) Reference third instar larval stage wing disc (Struhl and Basler, 1993) imaginal disc (Struhl and Basler, 1993)
Additional Information
Statement Reference Present in clones in animals carrying the progenitor transposon P{Act5C(FRT.polyA)lacZ.nls1} and exposed to a source of Scer\FLP1. (Struhl and Basler, 1993)

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	P{Act5C(FRT.polyA)lacZ.nls1} (Struhl and Basler, 1993)
descendant(s)
Comments
	construct_comment: Contains single FRT site. (Struhl and Basler, 1993) report comment: lacZ allele contains a nuclear localization signal. (Struhl and Basler, 1993)
Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	act5c>lacZ (Weigmann and Cohen, 1999) Act5C>nuc-lacZ (Struhl and Basler, 1993) act5c lacZ (Emerald and Cohen, 2004) Act>stop>lacZ (de la Cova et al., 2004) Actin>stop>lacZ (Hasegawa et al., 2011) P{Act5C(-FRT)lacZ.nls1} (FlyBase, 1996-) P{Act5C-nls-lacZ} (FlyBase, 1996-) P{lacZ.Act5C.S} (FlyBase, 1996-)
Secondary FlyBase IDs

References ( 19 )
Research paper	Hasegawa et al., 2011, Development 138(5): 983--993 Concentric zones, cell migration and neuronal circuits in the Drosophila visual center. [FBrf0213020] Morrison and Halder, 2010, genesis 48(1): 3--7 Characterization of a Dorsal-Eye Gal4 Line in Drosophila. [FBrf0209713] Lee et al., 2006, Genes Dev. 20(24): 3464--3474 Drosophila Aurora-A kinase inhibits neuroblast self-renewal by regulating aPKC/Numb cortical polarity and spindle orientation. [FBrf0195248] Baena-Lopez et al., 2005, Curr. Biol. 15(18): 1640--1644 The orientation of cell divisions determines the shape of Drosophila organs. [FBrf0187296] Jung et al., 2005, Development 132(11): 2521--2533 The Drosophila lymph gland as a developmental model of hematopoiesis. [FBrf0187467] de la Cova et al., 2004, Cell 117(1): 107--116 Drosophila myc regulates organ size by inducing cell competition. [FBrf0174444] Emerald and Cohen, 2004, Dev. Biol. 267(2): 462--472 Spatial and temporal regulation of the homeotic selector gene Antennapedia is required for the establishment of leg identity in Drosophila. [FBrf0174519] Vegh and Basler, 2003, Genetics 163(4): 1427--1438 A genetic screen for hedgehog targets involved in the maintenance of the Drosophila anteroposterior compartment boundary. [FBrf0158983] Rozowski and Akam, 2002, Genes Dev. 16(9): 1150--1162 Hox gene control of segment-specific bristle patterns in Drosophila. [FBrf0147040] Tomoyasu et al., 2000, Mech. Dev. 96(1): 37--49 The Decapentaplegic morphogen gradient regulates the notal wingless expression through induction of pannier and u-shaped in Drosophila. [FBrf0130117] Rangarajan et al., 1999, Development 126(15): 3285--3292 Migration and function of glia in the developing Drosophila eye. [FBrf0111468] Sun and Tower, 1999, Mol. Cell. Biol. 19(1): 216--228 FLP recombinase-mediated induction of Cu/Zn-superoxide dismutase transgene expression can extend the life span of adult Drosophila melanogaster flies. [FBrf0105941] Weigmann and Cohen, 1999, Development 126(17): 3823--3830 Lineage-tracing cells born in different domains along the PD axis of the developing Drosophila leg. [FBrf0111516] Woodhouse et al., 1998, Dev. Genes Evol. 207(8): 542--550 Growth, metastasis, and invasiveness of Drosophila tumors caused by mutations in specific tumor suppressor genes. [FBrf0102027] Reddy et al., 1997, Development 124(3): 703--712 Development of the Drosophila olfactory sense organs utilizes cell-cell interactions as well as lineage. [FBrf0092664] Perez and Steller, 1996, J. Neurobiol. 30(3): 359--373 Migration of glial cells into retinal axon target field in Drosophila melanogaster. [FBrf0089776] Struhl and Basler, 1993, Cell 72(4): 527--540 Organizing activity of wingless protein in Drosophila. [FBrf0057889]
Supplementary material	Baena-Lopez et al., 2005, Curr. Biol. 15(18): Supplemental Data. [FBrf0191744]
Letter	Moreno and Morata, 1999, Nature 400(6747): 873--877 Caudal is the Hox gene that specifies the most posterior Drosophile segment. [FBrf0111436]