Recombinant construct P{CXY21}

General Information
Symbol	P{CXY21}	FlyBase ID	FBtp0001113
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	1 available
Molecular map
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Description & Uses
Species	synthetic construct
Location-dependent role	carries FRT site(s) (Harrison and Perrimon, 1993)
Description	CV term Qualifiers & info Reference transposon P-element (Harrison and Perrimon, 1993)
Uses	CV term Qualifiers & info Reference analytical tool mosaic analysis (Harrison and Perrimon, 1993)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference chimeric gene (Harrison and Perrimon, 1993) reporter gene (Harrison and Perrimon, 1993) selectable marker (Harrison and Perrimon, 1993) selectable marker (Harrison and Perrimon, 1993)
Component Alleles
Allele	Ecol\lacZ^{Scer\FRT.NLS.αTub84B}
Reference(s)	(Harrison and Perrimon, 1993, Lawrence et al., 2002, Dominguez et al., 1998, Gonczy and DiNardo, 1996, Morimoto et al., 1996)
Molecular data	Construct: Ecol\lacZ protein coding region fused to 2kb of αTub84B promoter, including the 5' leader, intron and coding sequence for the first 21 amino acids. SV40 nuclear localisation signal is ligated in the sense orientation at the αTub84B Ecol\lacZ junction. Scer\FRT site is inserted within the αTub84B intron. w sequences are adjacent to Ecol\lacZ coding region and y sequences are adjacent to αTub84B promoter. (Harrison and Perrimon, 1993)
Phenotypic class
Phenotype manifest in
Other information

Allele	w^+mC
Reference(s)	(Harrison and Perrimon, 1993)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information

Allele	y^+mDint2
Reference(s)	(Harrison and Perrimon, 1993)
Molecular data	Construct: 5.2kb y⁺ gene including 2.8kb of 5' flanking sequences and 0.13kb of 3' sequences and lacks the tissue-specific bristle and tarsal claw enhancers. (Roseman et al., 1995) 5.2kb y gene fragment containing 2.8kb of 5' flanking and 0.13kb of 3' flanking DNA and lacking the intron that is the location of the tissue-specific bristle and tarsal claw enhancers. (Roseman et al., 1995) 5kb intronless SalI fragment containing the y gene. (Harrison and Perrimon, 1993) 5.2kb SalI y gene intronless fragment containing 2.9kb of 5' flanking and 0.3kb of 3' flanking DNA. (Patton et al., 1992)
Phenotypic class	body color defective (Ring and Garza, 2003.6.10, Roseman et al., 2001) visible (Roseman et al., 2001)
Phenotype manifest in	adult cuticle (Roseman et al., 2001) arista (Ring and Garza, 2003.6.10) macrochaeta (Ring and Garza, 2003.6.10) unguis (Ring and Garza, 2003.6.10)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	pP{CXY21} (Harrison and Perrimon, 1993)
descendant(s)	P{5'CXY21.29} (Harrison and Perrimon, 1993)
Comments
	construct_comment: Mosaic analysis requires initial generation (by imprecise excision events) of reciprocal derivatives of P{CXY21} at a given insertion site. (Harrison and Perrimon, 1993)
Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	CXY21 (Harrison and Perrimon, 1993) lacZy⁺ (Lawrence et al., 2002) P{CXY21} (FlyBase, 1996-)
Secondary FlyBase IDs

References ( 5 )
Research paper	Lawrence et al., 2002, Development 129(11): 2749--2760 Towards a model of the organisation of planar polarity and pattern in the Drosophila abdomen. [FBrf0148964] Dominguez et al., 1998, Curr. Biol. 8(19): 1039--1048 Multiple functions of the EGF receptor in Drosophila eye development. [FBrf0104744] Gonczy and DiNardo, 1996, Development 122(8): 2437--2447 The germ line regulates somatic cyst cell proliferation and fate during Drosophila spermatogenesis. [FBrf0089662] Morimoto et al., 1996, Development 122(12): 3745--3754 Pointed, an ETS domain transcription factor, negatively regulates the EGF receptor pathway in Drosophila oogenesis. [FBrf0091294] Harrison and Perrimon, 1993, Curr. Biol. 3(7): 424--433 Simple and efficient generation of marked clones in Drosophila. [FBrf0057940]