Open Close
General Information
Symbol
P{vg(D/V)-lacZ}
FlyBase ID
FBtp0001124
Feature type
transgenic_transposable_element
Component Allele(s)
Also Known As
vgBE-lacZ, vgBE-lacZ, vg-lacZ, vg-BE, vg BE-lacZ, vg-BE-lacZ, vgBE, vgB-lacZ
Size
Expression Data
lacZ reporter
Associated insertion(s)
Molecular map
Description and Uses
Location-dependent role
Description
CV term
Qualifiers and info
Reference
Construct components
Product class / Tool use(s)
Marker(s)
Component allele Ecol\lacZvg.int2.1
Product class / Tool use(s)
Encoded product / tool
Description

lacZ gene expression is driven by a 750bp EcoRI fragment from vg intron 2 (the vg intron 2 enhancer element) that shares sequence homology with the same fragment from Dvir\vg.

lacZ expression is governed by the wild-type vg second intron D/V boundary enhancer element.

Component allele w+mC
Product class / Tool use(s)
Regulatory region(s)
Description

4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site.

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Features
CV term
Qualifiers and info
Reference
chimeric
gene
reporter
gene
selectable
marker
Component Alleles
Molecular data

lacZ expression is governed by the wild-type vg second intron D/V boundary enhancer element.

lacZ gene expression is driven by a 750bp EcoRI fragment from vg intron 2 (the vg intron 2 enhancer element) that shares sequence homology with the same fragment from Dvir\vg.

Allele
Reference(s)
Molecular data

4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site.

Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Information
Statement
Reference

Ecol\lacZvg.int2.1 transcript is expressed starting at the beginning of the second larval instar in the wing disc. Ecol\lacZvg.int2.1 expression is observed along the D/V boundary of the wing disc, as well as along the part of the A/P boundary that lies within the wing pouch.

Expression restricted to a stripe of cells spanning the dorsal/ventral boundary in the wing and haltere discs. No expression observed in any other larval or embryonic tissues. Earliest expression in second instar wing disc is weak and diffuse; by late second instar has resolved into a broad stripe along the D/V boundary.

 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
Comments

construct_comment: One of a series of reporter constructs testing vg intronic enhancer elements (using Hsp70 promoter).

report comment: Contains the vg D/V boundary enhancer, including binding site of the su(H) protein.

Stocks (0)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (22)
References (66)