FlyBase Recombinant Construct Report: P{vg(D/V)-lacZ}

General Information

Symbol

P{vg(D/V)-lacZ}

FlyBase ID

FBtp0001124

Feature type

transgenic_transposable_element

Component Allele(s)

Ecol\lacZ^vg.int2.1

Also Known As

vg^BE-lacZ, vgBE-lacZ, vg-lacZ, vg-BE, vg BE-lacZ, vg-BE-lacZ, vgBE, vgB-lacZ

Size

Expression Data

lacZ reporter

Associated insertion(s)

1 available

Molecular map

Description and Uses

Construct components

Component

Use(s)

Marker(s)

w^+*

Component allele Ecol\lacZ^vg.int2.1

Transgenic product class

Component

Use(s)

Regulatory region(s)

vg_boundary_enhancer

Encoded product / tool

lacZ

reporter enzyme

Description

lacZ gene expression is driven by a 750bp EcoRI fragment from vg intron 2 (the vg intron 2 enhancer element) that shares sequence homology with the same fragment from Dvir\vg.

(Williams et al., 1994)

lacZ expression is governed by the wild-type vg second intron D/V boundary enhancer element.

(Kim et al., 1996)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Phenotypic Data

Component Allele

Phenotypic Data

Ecol\lacZ^vg.int2.1

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

second instar larval stage

wing disc | restricted

(Williams et al., 1994)

third instar larval stage

haltere disc | restricted

(Williams et al., 1994)

wing disc | restricted

(Williams et al., 1994)

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

second instar larval stage -- third instar larval stage

dorsal-ventral compartment boundary of the wing disc

(Neumann and Cohen, 1996)

anterior-posterior compartment boundary of the wing disc of wing pouch

(Neumann and Cohen, 1996)

Additional Information

Statement

Reference

EcollacZ^vg.int2.1 transcript is expressed starting at the beginning of the second larval instar in the wing disc. EcollacZ^vg.int2.1 expression is observed along the D/V boundary of the wing disc, as well as along the part of the A/P boundary that lies within the wing pouch.

(Neumann and Cohen, 1996)

Expression restricted to a stripe of cells spanning the dorsal/ventral boundary in the wing and haltere discs. No expression observed in any other larval or embryonic tissues. Earliest expression in second instar wing disc is weak and diffuse; by late second instar has resolved into a broad stripe along the D/V boundary.

(Williams et al., 1994)

Marker for

Reflects expression of

(Neumann and Cohen, 1996)

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

pP{vg(D/V)-lacZ}

(Kim et al., 1996, Williams et al., 1994)

descendant(s)

Comments

construct_comment: One of a series of reporter constructs testing vg intronic enhancer elements (using Hsp70 promoter).

(Williams et al., 1994)

report comment: Contains the vg D/V boundary enhancer, including binding site of the su(H) protein.

(Kim et al., 1996)

Stocks (0)

Stocks containing insertion(s) of this recombinant construct

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (23)

Reported As

Symbol Synonym

BE-lacZ

(Zecca and Struhl, 2007)

P{vg(D/V)-lacZ}

(FlyBase, 1996-)

P{vg(int2.1)-lacZ}

(FlyBase, 1996-)

P{vg-lacZ}

(FlyBase, 1996-)

Vg-boundary-lacZ

(Wang and Struhl, 2004)

mini-w⁺:vg^en

(Kim et al., 1995)

vestigial D/V enhancer-lacZ

(Ye et al., 1999)

vg BE-lacZ

(Baena-Lopez and Garcia-Bellido, 2003, Nagel et al., 2001)

vg boundary enhancer-lacZ

(Yun et al., 1999)

vg(B)

(Baonza and Garcia-Bellido, 2000)

vg(boundary)-lacZ

(Apidianakis et al., 1999)

vg-BE

(Kolzer et al., 2003, Prasad et al., 2003)

vg-BE-lacZ

(Pérez et al., 2011, Nagaraj et al., 1999)

vg-BElacZ

(Kugler and Nagel, 2010)

vg-DV enhancer-lacZ

(Bajpai et al., 2004)

vg-lacZ

(Mouri et al., 2014, Rincon-Limas et al., 1999, Varadarajan and VijayRaghavan, 1999)

vg^BE-LacZ

(Maier et al., 2011, Legent et al., 2006, Nagel et al., 2005, Mazaleyrat et al., 2003)

vg^BE-lac Z

(Maier et al., 2011)

vg^BE-lacZ

(Yuan et al., 2016, Brockmann et al., 2014, Xie et al., 2012, Mukai et al., 2010, Lee et al., 2005, Kamimura et al., 2004, Okajima and Irvine, 2002, Stewart et al., 2001, Furriols and Bray, 2000)

vgB-lacZ

(Grieder et al., 2009, Grieder et al., 2007)

vgBE

(Koelzer and Klein, 2006, Klein and Martinez Arias, 1999)

vgBE-lacZ

(Sasamura et al., 2013, Kugler and Nagel, 2007, Fuwa et al., 2006, Delanoue et al., 2004, Hori et al., 2004, Murata et al., 2001, Nolo et al., 2001, Casares and Mann, 2000, Ju et al., 2000, Lawrence et al., 2000)

vgBElacZ

(Farfán-Pira et al., 2022)

Secondary FlyBase IDs

References (69)

Report Sections

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