Recombinant construct P{70FLP}

General Information
Symbol	P{70FLP}	FlyBase ID	FBtp0001131
Feature type	transgenic_transposon	Created / Updated	1997-03-22/1998-07-06

Size		Expression data
Location-dependent role		Associated insertions	7 available
Molecular map
Description & Uses
Species	synthetic construct
Description	CV term Qualifiers & info Reference transposon P-element (Golic, 1994, Golic and Golic, 1996, Golic et al., 1997)
Uses	CV term Qualifiers & info Reference enzyme source Scer\FLP1 (Golic, 1994)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Sequence
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference inducible promoter (Golic and Golic, 1996) general promoter (Golic and Golic, 1996) chimeric gene (Golic and Golic, 1996) selectable marker (Golic and Golic, 1996)
Component Alleles
ry^+t7.2
Reference(s)	(Golic and Golic, 1996, Golic, 1994)
Molecular data	Construct: 7.2kb HindIII fragment that contains ry⁺ function. (Rubin and Spradling, 1983)
Phenotypic class	wild-type (Rubin and Spradling, 1983)
Phenotype manifest in
Other information
Scer\FLP1^hs.PP
Reference(s)	(Golic and Golic, 1996, Ahmad and Golic, 1998, Kracklauer et al., 2007, Watnick et al., 2003, Gao et al., 2003, Seum et al., 2002, Rong and Golic, 2001, Golic et al., 1997, Golic and Golic, 1996, Gao et al., 2003, Watnick et al., 2003, Lankenau et al., 2003, Ahmad and Golic, 1996, Sogame et al., 2003)
Molecular data	Construct: Similar to Scer\FLP1^hs.PG in that the Scer\FLP1 recombinase gene expression is driven by an Hsp70 promoter, but 3' noncoding sequences from an Hsp70 gene have been substituted for the yeast Scer\FLP1 3' sequence. (Golic and Golic, 1996)
Phenotypic class
Phenotype manifest in
Other information
Expression Data
Reporter Expression
Stage Tissue/Position Reference
Additional Information
Statement Reference

Assay mode
Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	pP{70FLP} (Golic and Golic, 1996)
descendant(s)
Comments
	report comment: Shows higher levels of expression after heat-shock induction than does P{hsFLP}. (Golic and Golic, 1996) report comment: One of several Hsp70-FLP1 constructs.Described as similar to P{hsFLP}. (Golic, 1994)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	70FLP (Gao et al., 2003, Watnick et al., 2003, Gao et al., 2003, Watnick et al., 2003, Sogame et al., 2003, Rong and Golic, 2001) hs-FLP (Kracklauer et al., 2007) P[ry⁺,70FLP] (Seum et al., 2002) P[ry⁺, 70FLP] (Golic and Golic, 1996) P{70FLP} (FlyBase, 1996-, Golic et al., 1997) P{@FLP1^hs.PP@ @ry^+t7.2@=@70FLP@} (FlyBase, 1996-) P{ry⁺,70FLP} (Lankenau et al., 2003)
Secondary FlyBase IDs

References ( 17 )
Research paper	Kracklauer et al., 2007, Fly 1(2): 75--85 Drosophila klaroid Encodes a SUN Domain Protein Required for Klarsicht Localization to the Nuclear Envelope and Nuclear Migration in the Eye. [FBrf0200125] Gao et al., 2003, Curr. Biol. 13(24): 2175--2178 PKD2 cation channel is required for directional sperm movement and male fertility. [FBrf0167414] Lankenau et al., 2003, Genetics 163(2): 611--623 Knockout targeting of the Drosophila Nap1 gene and examination of DNA repair tracts in the recombination products. [FBrf0158984] Sogame et al., 2003, Proc. Natl. Acad. Sci. USA 100(8): 4696--4701 Drosophila p53 preserves genomic stability by regulating cell death. [FBrf0159299] Watnick et al., 2003, Curr. Biol. 13(24): 2179--2184 A flagellar polycystin-2 homolog required for male fertility in Drosophila. [FBrf0167415] Seum et al., 2002, Genetics 161(3): 1125--1136 Isolation of Su(var)3-7 mutations by homologous recombination in Drosophila melanogaster. [FBrf0151329] Rong and Golic, 2001, Genetics 157(3): 1307--1312 A targeted gene knockout in Drosophila. [FBrf0134649] Ahmad and Golic, 1998, Genetics 148(2): 775--792 The transmission of fragmented chromosomes in Drosophila melanogaster. [FBrf0101865] Golic et al., 1997, Nucleic Acids Res. 25(18): 3665--3671 FLP-mediated DNA mobilization to specific target sites in Drosophila chromosomes. [FBrf0098247] Ahmad and Golic, 1996, Genetics 144(2): 657--670 Somatic reversion of chromosomal position effects in Drosophila melanogaster. [FBrf0090428] Golic and Golic, 1996, Genetics 144(4): 1693--1711 Engineering the Drosophila genome: chromosome rearrangements by design. [FBrf0091061] Golic and Golic, 1996, Genetics 143(1): 385--400 A quantitative measure of the mitotic pairing alleles in Drosophila melanogaster and the influence of structural heterozygosity. [FBrf0087226] Golic, 1994, Genetics 137(2): 551--563 Local transposition of P elements in Drosophila melanogaster and recombination between duplicated elements using a site-specific recombinase. [FBrf0073185] Rubin and Spradling, 1983, Nucleic Acids Res. 11(18): 6341--6351 Vectors for P element-mediated gene transfer in Drosophila. [FBrf0040064]
Supplementary material	Gao et al., 2003, Curr. Biol. 13(24): PKD2 cation channel is required for directional sperm movement and male fertility. [FBrf0178917] Watnick et al., 2003, Curr. Biol. 13(24): A flagellar polycystin-2 homolog required for male fertility in Drosophila. [FBrf0178959]
FlyBase analysis	FlyBase, 1996-, FlyBase inference based on genome sequence analysis. FlyBase inference based on genome sequence analysis. [FBrf0104946]