Recombinant construct P{hsp70-PKA.A901}

General Information
Symbol	P{hsp70-PKA.A901}	FlyBase ID	FBtp0001138
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	2 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Jiang and Struhl, 1995)
Uses	CV term Qualifiers & info Reference characterization Mmus\Pkaca (Jiang and Struhl, 1995)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference general promoter (Jiang and Struhl, 1995) inducible promoter (Jiang and Struhl, 1995)
Component Alleles
Allele	Mmus\Pkaca^hs.PJ
Reference(s)	(Jiang and Struhl, 1995, Williams et al., 2001, Williams et al., 2001, Guo et al., 2000, Asha et al., 1999, The et al., 1997, Tong et al., 2007)
Molecular data	Construct: The coding sequence of the catalytic subunit of Mmus\Pkaca with amino acid substitutions: H87Q and W196R. Expression of the mutated subunit is driven by an Hsp70 promoter. (Jiang and Struhl, 1995)
Phenotypic class	locomotor rhythm defective (Williams et al., 2001, Williams et al., 2001)
Phenotype manifest in
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	pP{hsp70-PKA.A901} (Jiang and Struhl, 1995)
descendant(s)
Comments
	report comment: The mouse PKA allele carried in this construct is constitutively active (reduced binding to regulatory subunit).Selectable marker not specified. (Jiang and Struhl, 1995)
Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	hsp70-PKA* (Guo et al., 2000, Asha et al., 1999) hsPKA (Williams et al., 2001, Tong et al., 2007) P{hsp70-PKA.A901} (FlyBase, 1996-) PKA* (Williams et al., 2001)
Secondary FlyBase IDs

References ( 7 )
Research paper	Tong et al., 2007, Nat. Genet. 39(4): 476--485 Life extension through neurofibromin mitochondrial regulation and antioxidant therapy for neurofibromatosis-1 in Drosophila melanogaster. [FBrf0200172] Williams et al., 2001, Science 293(5538): 2251--2256 A circadian output in Drosophila mediated by Neurofibromatosis-1 and Ras/MAPK. [FBrf0138535] Guo et al., 2000, Nature 403(6772): 895--898 A neurofibromatosis-1-regulated pathway is required for learning in Drosophila. [FBrf0125468] Asha et al., 1999, EMBO J. 18(3): 605--615 The Rap1 GTPase functions as a regulator of morphogenesis in vivo. [FBrf0106166] The et al., 1997, Science 276(5313): 791--794 Rescue of a Drosophila NF1 mutant phenotype by protein kinase A. [FBrf0093751] Jiang and Struhl, 1995, Cell 80(4): 563--572 Protein kinase A and hedgehog signaling in Drosophila limb development. [FBrf0080131]
Supplementary material	Williams et al., 2001, Science 293(5538): A circadian output in Drosophila mediated by Neurofibromatosis-1 and Ras/MAPK. [FBrf0141792]