Recombinant construct P{GAL4-vg.M}

General Information
Symbol	P{GAL4-vg.M}	FlyBase ID	FBtp0001163
Feature type	transgenic_transposon

Size		Expression data	GAL4 reporter/driver
Associated insertions	1 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Simmonds et al., 1995)
Uses	CV term Qualifiers & info Reference binary system (regulatory) vg (Simmonds et al., 1995) characterization vg \| regulatory (Simmonds et al., 1995)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference binary component (Simmonds et al., 1995) reporter gene (Simmonds et al., 1995)
Component Alleles
Allele	Scer\GAL4^vg.PM
Reference(s)	(Collins et al., 1999, Janody et al., 2003, Prasad et al., 2003, MacKay et al., 2003, Szuplewski et al., 2003, Bokel et al., 2006, Fouix et al., 2003, Nakagoshi et al., 2002, Parker et al., 2002, Bourouis, 2002, Srivastava et al., 2002, Maurange and Paro, 2002, Baena-Lopez and Garcia-Bellido, 2003, Igaki et al., 2002, Belenkaya et al., 2002, Han et al., 2004, Bajpai et al., 2004, Caygill and Johnston, 2008, Jaiswal et al., 2006, Lee and Baehrecke, 2001, Wan et al., 2000, Collins and Treisman, 2000, Lee et al., 2000, Chen and Struhl, 1999, Shashidhara et al., 1999, Schweizer and Basler, 1998, Strigini and Cohen, 1997, Tsuneizumi et al., 1997, Rorth et al., 2000, Chen et al., 2004, Bajpai et al., 2004, Kataoka et al., 2001, Lecuit et al., 1996, Bennett et al., 2003, Simmonds et al., 1995, Pallavi and Shashidhara, 2003, Mohit et al., 2006, Oh and Irvine, 2009, Lawrence et al., 2004, Miura et al., 2006, Israeli et al., 2007, Brun et al., 2006, Loya et al., 2009, Zhang et al., 2011, Casso et al., 2008, Lu et al., 2009, Grönke et al., 2009, Bischoff et al., 2009, Mallik and Lakhotia, 2009, Usha and Shashidhara, 2010, Gaumer et al., 2000, Colin et al., 2009, Jacobsson et al., 2009, Yu et al., 2013, Vyas et al., 2008)
Molecular data	Construct: vg promoter drives the expression of Scer\GAL4. (Simmonds et al., 1995)
Phenotypic class	hyperplasia, with stwl^UY823 (Brun et al., 2006) increased cell death, with Eip93F^Scer\UAS.cLa (Lee et al., 2000) increased cell death, with Mmus\Bax^Scer\UAS.cGa (Colin et al., 2009) lethal, with Mmus\Bax^Scer\UAS.cGb (Gaumer et al., 2000) lethal, with yki^{S111A.S168A.S250A.Scer\UAS.T:SV5\V5} (Oh and Irvine, 2009) lethal, with yki^{S111A.S168A.Scer\UAS.T:SV5\V5} (Oh and Irvine, 2009) lethal, with yki^{S168A.S250A.Scer\UAS.T:SV5\V5} (Oh and Irvine, 2009) lethal, with yki^{S168A.Scer\UAS.T:SV5\V5} (Oh and Irvine, 2009) lethal \| pupal stage, with Eip93F^Scer\UAS.cLa (Lee et al., 2000) lethal \| pupal stage \| heat sensitive, with stwl^UY823 (Brun et al., 2006) viable, with A2bp1^{dsRNA.Scer\UAS} (Usha and Shashidhara, 2010) visible, with AGO1^Scer\UAS.cKa (Kataoka et al., 2001) visible, with brm^{K804R.Scer\UAS.T:Ivir\HA1} (Collins et al., 1999) visible, with CG3632^Scer\UAS.cYa (Yu et al., 2013) visible, with ci::pan^ciD.Scer\UAS (Schweizer and Basler, 1998) visible, with ci::pan^{S28N.ciD.Scer\UAS} (Schweizer and Basler, 1998) visible, with ct^{dsRNA.cGa.Scer\UAS} (Casso et al., 2008) visible, with Dad^Scer\UAS.cTa (Tsuneizumi et al., 1997) visible, with dve^Scer\UAS.cNa (Nakagoshi et al., 2002) visible, with gbb^Scer\UAS.cUa (Yu et al., 2013) visible, with hh^{Scer\UAS.T:Avic\GFP-EGFP.cVa} (Vyas et al., 2008) visible, with Hsap\MTMR4^Scer\UAS.cYa (Yu et al., 2013) visible, with kto^Scer\UAS.cJa (Janody et al., 2003) visible, with let-7^Scer\UAS.cCa (Caygill and Johnston, 2008) visible, with Mad^Scer\UAS.cNa (Tsuneizumi et al., 1997) visible, with Mlf^Scer\UAS.cFa (Fouix et al., 2003) visible, with osa^Scer\UAS.cCa (Collins et al., 1999) visible, with put^Scer\UAS.cMa (Bennett et al., 2003) visible, with Rbf^Scer\UAS.cDa (Szuplewski et al., 2003) visible, with sgg^S9A.Scer\UAS (Bourouis, 2002) visible, with sgg^S9E.Scer\UAS (Bourouis, 2002) visible, with sgg^Scer\UAS.cBa (Bourouis, 2002) visible, with shg^Scer\UAS.cOa (Kataoka et al., 2001) visible, with skd^Scer\UAS.cJa (Janody et al., 2003) visible, with slbo^Scer\UAS.cRa (Rorth et al., 2000) visible, with tkv^{dsRNA.Scer\UAS.cUa} (Yu et al., 2013) visible, with tkv^{Q253D.Scer\UAS.cNb} (Tsuneizumi et al., 1997) visible, with Ubx^Scer\UAS.cCa (Bischoff et al., 2009) visible, with vri^1.Scer\UAS (Szuplewski et al., 2003)
Phenotype manifest in	alula, with rpr^Scer\UAS.C (Mallik and Lakhotia, 2009) axillary cell, with rpr^Scer\UAS.C (Mallik and Lakhotia, 2009) macrochaeta, with sd::vg^{Scer\UAS.sdTEA} (Srivastava et al., 2002) mesothoracic tergum \| ectopic, with sgg^S9A.Scer\UAS (Bourouis, 2002) mesothoracic tergum \| ectopic, with sgg^S9E.Scer\UAS (Bourouis, 2002) mesothoracic tergum \| ectopic, with sgg^Scer\UAS.cSa (Bajpai et al., 2004) peripodial epithelium & dorsal mesothoracic disc, with Ubx^Scer\UAS.cCa (Pallavi and Shashidhara, 2003) scutellum, with sd::vg^{Scer\UAS.sdTEA} (Srivastava et al., 2002) thorax, with sd::vg^{Scer\UAS.sdTEA} (Srivastava et al., 2002) wing, with brm^{K804R.Scer\UAS.T:Ivir\HA1} (Collins et al., 1999) wing, with Dad^Scer\UAS.cTa (Tsuneizumi et al., 1997) wing, with dve^Scer\UAS.cNa (Nakagoshi et al., 2002) wing, with egr^GS9830 (Igaki et al., 2002) wing, with egr^Scer\UAS.cIa (Mallik and Lakhotia, 2009) wing, with Fmr1^Scer\UAS.cWa (Wan et al., 2000) wing, with hh^{Scer\UAS.T:Avic\GFP-EGFP.cVa} (Vyas et al., 2008) wing, with let-7^Scer\UAS.cCa (Caygill and Johnston, 2008) wing, with Mad^Scer\UAS.cNa (Tsuneizumi et al., 1997) wing, with Mmus\Bax^Scer\UAS.cGa (Colin et al., 2009) wing, with osa^Scer\UAS.cCa (Collins et al., 1999) wing, with pan^ΔN.Scer\UAS (Bajpai et al., 2004) wing, with put^Scer\UAS.cMa (Bennett et al., 2003) wing, with rpr^Scer\UAS.cIa (Igaki et al., 2002) wing, with sgg^S9A.Scer\UAS (Collins and Treisman, 2000, Bourouis, 2002) wing, with sgg^S9E.Scer\UAS (Bourouis, 2002) wing, with sgg^Scer\UAS.cSa (Bajpai et al., 2004) wing, with slbo^Scer\UAS.cRa (Rorth et al., 2000) wing, with tkv^{Q253D.Scer\UAS.cNb} (Tsuneizumi et al., 1997) wing, with Zzzz\EF^Scer\UAS.cGa (Guichard et al., 2006) wing \| anterior \| proximal, with en^Scer\UAS.cSa (Simmonds et al., 1995) wing \| anterior compartment, with en^Scer\UAS.cSa (Simmonds et al., 1995) wing \| posterior compartment, with en^Scer\UAS.cSa (Simmonds et al., 1995) wing \| temperature conditional, with Mmus\Bax^Scer\UAS.cGb (Gaumer et al., 2000) wing blade, with Ubx^Scer\UAS.cCa (Bischoff et al., 2009) wing cell, with Fmr1^Scer\UAS.cWa (Wan et al., 2000) wing disc, with Eip93F^Scer\UAS.cLa (Lee et al., 2000) wing disc, with let-7^Scer\UAS.cCa (Caygill and Johnston, 2008) wing disc, with sgg^Scer\UAS.cSa (Mohit et al., 2006) wing disc, with stwl^UY823 (Brun et al., 2006) wing disc \| temperature conditional, with Mmus\Bax^Scer\UAS.cGb (Gaumer et al., 2000) wing hinge, with osa^Scer\UAS.cCa (Collins et al., 1999) wing hinge, with stwl^UY823 (Brun et al., 2006) wing hinge primordium, with stwl^UY823 (Brun et al., 2006) wing margin, with aos::shg^{i.Scer\UAS.T:Hsap\MYC} (Bajpai et al., 2004) wing margin, with ct^{dsRNA.cGa.Scer\UAS} (Casso et al., 2008) wing margin, with Fmr1^Scer\UAS.cWa (Wan et al., 2000) wing margin, with kto^Scer\UAS.cJa (Janody et al., 2003) wing margin, with Mlf^Scer\UAS.cFa (Fouix et al., 2003) wing margin, with Mmus\Bax^Scer\UAS.cGa (Colin et al., 2009) wing margin, with pan^ΔN.Scer\UAS (Bajpai et al., 2004) wing margin, with Rbf^Scer\UAS.cDa (Szuplewski et al., 2003) wing margin, with sgg^S9A.Scer\UAS (Collins and Treisman, 2000) wing margin, with sgg^S9E.Scer\UAS (Bourouis, 2002) wing margin, with sgg^Scer\UAS.cBa (Bourouis, 2002) wing margin, with sgg^Scer\UAS.cSa (Bajpai et al., 2004) wing margin, with shg^Scer\UAS.cOa (Kataoka et al., 2001) wing margin, with skd^Scer\UAS.cJa (Janody et al., 2003) wing margin, with stwl^UY823 (Brun et al., 2006) wing margin, with Ube3a^Scer\UAS.cLa (Lu et al., 2009) wing margin, with vri^1.Scer\UAS (Szuplewski et al., 2003) wing margin bristle, with ci::pan^ciD.Scer\UAS (Schweizer and Basler, 1998) wing margin bristle, with ci::pan^{S28N.ciD.Scer\UAS} (Schweizer and Basler, 1998) wing margin bristle, with sgg^Scer\UAS.cBa (Bourouis, 2002) wing margin bristle \| ectopic, with arm^{S10.Scer\UAS.T:Hsap\MYC} (Bajpai et al., 2004) wing margin bristle \| ectopic, with arm^{Δ.Scer\UAS.T:Ivir\HA1} (Bajpai et al., 2004) wing margin bristle \| ectopic, with dsh^Scer\UAS.cNa (Bajpai et al., 2004) wing margin bristle \| posterior \| proximal, with brm^{K804R.Scer\UAS.T:Ivir\HA1} (Collins et al., 1999) wing pouch, with stwl^UY823 (Brun et al., 2006) wing vein \| ectopic, with CG3632^Scer\UAS.cYa (Yu et al., 2013) wing vein \| ectopic, with gbb^Scer\UAS.cUa (Yu et al., 2013) wing vein \| ectopic, with Hsap\MTMR4^Scer\UAS.cYa (Yu et al., 2013) wing vein \| ectopic, with tkv^{dsRNA.Scer\UAS.cUa} (Yu et al., 2013)
Other information
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS) Stage Tissue/Position (including subcellular localization) Reference third instar larval stage wing disc \| restricted (Lecuit et al., 1996, Simmonds et al., 1995)
Additional Information
Statement Reference In wing disc (third larval instar), expressed in region of the presumptive wing blade. (Simmonds et al., 1995) In wing disc (third larval instar), expressed along dorsal/ventral compartment boundary. (Lecuit et al., 1996)

Marker for
Reflects expression of	vg (Simmonds et al., 1995)
Progenitors & Descendants
progenitor(s)	pP{GAL4-vg.M} (Simmonds et al., 1995)
descendant(s)
Comments
	construct_comment: Obtained from S.M. Morimura and F.M. Hoffmann.Selectable marker not specified. (Simmonds et al., 1995)
Stocks Listed in FlyBase ( 1 )
Bloomington	6819 w^*; P{GAL4-vg.M}2; TM2/TM6B, Tb¹
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{GAL4-vg.M} (FlyBase, 1996-, Casso et al., 2008, Grönke et al., 2009, Jacobsson et al., 2009) P{Gal4-vg-M} (Bischoff et al., 2009) vestigial-gal4 (Israeli et al., 2007) vestigial-Gal4 (Bischoff et al., 2009) vg.Gal4 (Lawrence et al., 2004) Vg(181)-Gal4 (Bourouis, 2002) vg^Q1206-Gal4 (Chen et al., 2004, Chen and Struhl, 1999) vg-G4 (Baena-Lopez and Garcia-Bellido, 2003) vg-GAL4 (Bajpai et al., 2004, Bajpai et al., 2004, Pallavi and Shashidhara, 2003, Szuplewski et al., 2003, Janody et al., 2003, Bennett et al., 2003, Prasad et al., 2003, Maurange and Paro, 2002, Igaki et al., 2002, Kataoka et al., 2001, Lee and Baehrecke, 2001, Wan et al., 2000, Lee et al., 2000, Collins et al., 1999, Shashidhara et al., 1999, FlyBase, 1996-, Mohit et al., 2006, Fouix et al., 2003, Miura et al., 2006, Mallik and Lakhotia, 2009, Usha and Shashidhara, 2010, Gaumer et al., 2000, Brun et al., 2006, Vyas et al., 2008) vgGAL4 (MacKay et al., 2003, Rorth et al., 2000) vg-GAl4 (Parker et al., 2002) vg-Gal4 (Srivastava et al., 2002, Collins and Treisman, 2000, Schweizer and Basler, 1998, Bokel et al., 2006, Oh and Irvine, 2009, Jaiswal et al., 2006, Lu et al., 2009, Loya et al., 2009) Vg-GAL4 (Simmonds et al., 1995) VgGal4 (Caygill and Johnston, 2008) vg-gal4 (Colin et al., 2009, Yu et al., 2013) vg-Gal4-181 (Nakagoshi et al., 2002) vgQ1206-Gal4 (Han et al., 2004) vg Q1206-Gal4 (Belenkaya et al., 2002, Zhang et al., 2011)
Secondary FlyBase IDs

References ( 55 )

Generate a list of	All references relating to this transgenic transposon ( 55 )

List References by type	Research paper ( 54 ) Supplementary material ( 1 )
Recent research papers ( 2 )
	Yu et al., 2013, J. Biol. Chem. 288(1): 79--88 Myotubularin-related Protein 4 (MTMR4) Attenuates BMP/Dpp Signaling by Dephosphorylation of Smad Proteins. [FBrf0220364] Zhang et al., 2011, Cell Res. 21(12): 1677--1690 SNX3 controls Wingless/Wnt secretion through regulating retromer-dependent recycling of Wntless. [FBrf0216871] Show all research papers ...