Recombinant construct P{αTub84B(FRT.y⁺CD2)en.Z2}

General Information
Symbol	P{αTub84B(FRT.y⁺CD2)en.Z2}	FlyBase ID	FBtp0001176
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	0 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Zecca et al., 1995, FlyBase, 1992-)
Uses	CV term Qualifiers & info Reference characterization en \| mosaic analysis (Zecca et al., 1995) FRT construct en (Zecca et al., 1995)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference cell marker (Zecca et al., 1995) chimeric gene (Zecca et al., 1995) FRT progenitor (Zecca et al., 1995) removable cassette (Zecca et al., 1995) selectable marker (Zecca et al., 1995)
Component Alleles
Allele	en^{Scer\FRT.Rnor\CD2.αTub84B}
Reference(s)	(Zecca et al., 1995, Lawrence et al., 1999, Strutt and Mlodzik, 1996)
Molecular data	Construct: en coding sequences (1-1840 of FBrf0042028) separated from αTub84B promoter by Scer\FRT sites flanking both Rnor\CD2^A902 and y^+t7.7. (Zecca et al., 1995)
Phenotypic class
Phenotype manifest in
Other information

Allele	Hsp70Ab^polyA
Reference(s)	(FlyBase, 1992-, Zecca et al., 1995)
Molecular data	Construct: 2.1kb SalI-EcoRI fragment initiating at the SalI site immediately 3' of the Hsp70Ab coding region, including the Hsp70Ab polyadenylation signal.
Phenotypic class
Phenotype manifest in
Other information

Allele	Rnor\CD2^A902
Reference(s)	(Zecca et al., 1995)
Molecular data	Construct: Rnor\CD2 coding sequence. (Jiang and Struhl, 1995)
Phenotypic class
Phenotype manifest in
Other information

Allele	y^+t7.7
Reference(s)	(Zecca et al., 1995)
Molecular data	Construct: 8kb SalI fragment carrying y⁺. (Merriam, 1997.5.9) Contained within 7.7kb SalI-BglII fragment that includes the entire y⁺ gene. (Struhl and Basler, 1993) Fragment containing the y gene, including 2873bp 5' and 130bp 3' flanking sequences. (Harrison et al., 1989) 5' regulatory region deletion derivative of y, 2873 nucleotides remain upstream of the transcription start site. (Geyer and Corces, 1987)
Phenotypic class	wild-type (Benedyk et al., 1994, Geyer and Corces, 1987, Harrison et al., 1989)
Phenotype manifest in
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)	P{αTub84B(-FRT)en.Z2} (Zecca et al., 1995)
Comments
	report comment: Allele en^αTub84B.PZ is the FRT-deleted (active) form, carried on descendant transposon P{Τub84B-en.Z2}}.CD2 expressed from the αTub84B promoter sequences. (Zecca et al., 1995)
Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{en.FRT.Z2} (FlyBase, 1996-) P{αTub84B(FRT.y⁺CD2)en.Z2} (FlyBase, 1996-) tub>y⁺>en (Lawrence et al., 1999) Tubα1>CD2,y⁺>en (Zecca et al., 1995)
Secondary FlyBase IDs

References ( 3 )
Research paper	Lawrence et al., 1999, Development 126(11): 2431--2439 hedgehog and engrailed: pattern formation and polarity in the Drosophila abdomen. [FBrf0108517] Strutt and Mlodzik, 1996, Mech. Dev. 58(1-2): 39--50 The regulation of hedgehog and decapentaplegic during Drosophila eye imaginal disc development. [FBrf0090819] Zecca et al., 1995, Development 121(8): 2265--2278 Sequential organizing activities of engrailed, hedgehog and decapentaplegic in the Drosophila wing. [FBrf0083533]

Recombinant construct P{αTub84B(FRT.y+CD2)en.Z2}

Recombinant construct P{αTub84B(FRT.y⁺CD2)en.Z2}