Recombinant construct P{UAS-tau-lacZ.B}

General Information
Symbol	P{UAS-tau-lacZ.B}	FlyBase ID	FBtp0001184
Feature type	transgenic_transposon

Size		Expression data	lacZ reporter
Associated insertions	2 available
Molecular map
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Hidalgo et al., 1995)
Uses	CV term Qualifiers & info Reference analytical tool cell marker (Hidalgo et al., 1995) binary system (targeted) Btau\MAPT (Hidalgo et al., 1995) Ecol\lacZ (Hidalgo et al., 1995) characterization Scer\UAS (Hidalgo et al., 1995) reporter construct Ecol\lacZ \| binary system (Hidalgo et al., 1995)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference binary component (Hidalgo et al., 1995) cell marker (Hidalgo et al., 1995) chimeric gene (Hidalgo et al., 1995) molecular tag (Hidalgo et al., 1995) selectable marker (Hidalgo et al., 1995)
Component Alleles
Allele	Ecol\lacZ^{Scer\UAS.T:Btau\MAPT}
Reference(s)	(Booth et al., 2000, Sepp et al., 2001, Sepp and Auld, 2003, Kurusu et al., 2002, Ward et al., 2002, Buch et al., 2008, Acebes et al., 2004, Garces and Thor, 2006, Gomez-Diaz et al., 2004, Siegmund and Korge, 2001, Takizawa and Hotta, 2001, Sotillos and de Celis, 2006, Jacobs et al., 2000, Baines et al., 1999, Martin-Bermudo et al., 1999, Landgraf et al., 1999, Llimargas and Casanova, 1999, Llimargas, 1999, Williams and Shepherd, 1999, Martinek and Young, 2000, Hidalgo and Brand, 1997, Hidalgo et al., 1995, Boube et al., 2001, Fujioka et al., 2003, Brand and Dormand, 1995, Martin-Bermudo et al., 1997, Buescher et al., 2006, Bossing and Brand, 2006, Pereanu and Hartenstein, 2006, Simionato et al., 2007, Lacin et al., 2009, Hassan et al., 2005, Lear et al., 2005, Rival et al., 2006, Strigini et al., 2006, Hiramoto and Hiromi, 2006)
Molecular data	Construct: Btau\MAPT-Ecol\lacZ gene fusion, including an SV40 terminator were excised as an XboI-XbaI fragment from P{etau-lacZ} is fused to Scer\GAL4 Scer\UAS binding site. (Hidalgo et al., 1995)
Phenotypic class	lethal \| male \| pupal stage, with Scer\GAL4^cb01 (Ward et al., 2002) lethal \| male \| pupal stage, with Scer\GAL4^cb07 (Ward et al., 2002) lethal \| male \| pupal stage, with Scer\GAL4^cb10 (Ward et al., 2002) lethal \| pupal stage, with Scer\GAL4^cb14 (Ward et al., 2002) lethal \| pupal stage, with Scer\GAL4^cb22 (Ward et al., 2002) lethal \| pupal stage, with Scer\GAL4^cb25 (Ward et al., 2002) lethal \| pupal stage, with Scer\GAL4^cb26 (Ward et al., 2002) lethal \| pupal stage, with Scer\GAL4^cb32 (Ward et al., 2002) lethal \| pupal stage, with Scer\GAL4^cb35 (Ward et al., 2002) lethal \| pupal stage, with Scer\GAL4^cb36 (Ward et al., 2002) lethal \| pupal stage, with Scer\GAL4^cb41 (Ward et al., 2002)
Phenotype manifest in
Other information

Allele	w^+mC
Reference(s)	(Hidalgo et al., 1995)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference Scer\GAL4-targeted reporter; β-galactosidase distribution reflects action of enhancer/promoter elements driving GAL4 in the same cell.Product localized to microtubules: carries binding domain of Btau\MAPT microtubule-associated protein. (Hidalgo et al., 1995) Scer\GAL4-targeted reporter: expression of Ecol\lacZ reflects action of enhancer/promoter elements driving GAL4 in the same cell.Product localized to microtubules; allows visualization of cell body, axons and other projections. (Hidalgo et al., 1995)

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	pP{UAS-tau-lacZ.B} (Hidalgo et al., 1995)
descendant(s)
Comments
	construct_comment: Btau\MAPT (tau) is a microtubule-associated protein; the MAPT-lacZ fusion protein associates with microtubules, allowing the entire cell to be visualized, including axons and other neural projections. (Hidalgo et al., 1995)
Stocks Listed in FlyBase ( 6 )
Bloomington	5829 y¹ w^; P{UAS-tau-lacZ.B}2 7469 w^; P{CQ2-GAL4}H, P{UAS-tau-lacZ.B}2 7471 w^; P{RN2-GAL4}E, P{UAS-tau-lacZ.B}3 7474 w^; P{RN2-GAL4}P, P{UAS-tau-lacZ.B}2 7467 y¹ w^*; P{CQ2-GAL4}O, P{UAS-tau-lacZ.B}3
Kyoto	108451 y¹ w^*; P{UAS-tau-lacZ.B}2
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	{UAS-tau-lacZ.B} (Buescher et al., 2006) P{UAS-btau.lacZ} (FlyBase, 1996-) P{UAS-lacZ.btau} (FlyBase, 1996-) P{UAS-tau-lacZ.B} (FlyBase, 1996-) tau-lacZ (Martinek and Young, 2000) UAS TAU/lacZ (Williams and Shepherd, 1999) UAS-tauLacZ (Acebes et al., 2004) UAS tau lacZ (Booth et al., 2000) UAS-tau-lacZ (Bossing and Brand, 2006, Garces and Thor, 2006, Hiramoto and Hiromi, 2006, Strigini et al., 2006) UAS-taulacZ (Sotillos and de Celis, 2006, Lear et al., 2005) UAS-taulacz (Pereanu and Hartenstein, 2006) UAS-tau-LacZ (Rival et al., 2006) UAS-tlacZ (Benveniste et al., 1998) UAS-τlacZ (Simionato et al., 2007) UAS-τLacZ (Buch et al., 2008) UAS-τ-lacZ (Lacin et al., 2009, Hassan et al., 2005)
Secondary FlyBase IDs

References ( 38 )

Generate a list of	All references relating to this transgenic transposon ( 38 )

List References by type	Research paper ( 37 ) Review ( 1 )
Recent research papers (0)
	All research papers listed in FlyBase were published before 2011 Show research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...