Recombinant construct P{UAS-tkv.Na}

General Information
Symbol	P{UAS-tkv.Na}	FlyBase ID	FBtp0001196
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	0 available
Molecular map
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Nellen et al., 1996)
Uses	CV term Qualifiers & info Reference binary system (targeted) tkv (Nellen et al., 1996) characterization tkv (Nellen et al., 1996)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference binary component (Nellen et al., 1996) chimeric gene (Nellen et al., 1996) selectable marker (Nellen et al., 1996)
Component Alleles
Allele	tkv^Scer\UAS.cNa
Reference(s)	(Crickmore and Mann, 2007, Myat et al., 2005, Reim and Frasch, 2005, Funakoshi et al., 2001, Tanimoto et al., 2000, Gibson and Perrimon, 2005, Shen and Dahmann, 2005, Kruse et al., 2004, Nellen et al., 1996, Vincent et al., 1997, Crickmore and Mann, 2006, Crickmore and Mann, 2006, Shen et al., 2008, Terriente et al., 2008, Widmann and Dahmann, 2009)
Molecular data	Construct: Expression of a 2.5kb EcoRI-DraI fragment of cDNA STK-A fused to αTub84B transcriptional termination sequences is influences by Scer\UAS Scer\GAL4 binding sites. (Nellen et al., 1996)
Phenotypic class	cell shape defective \| third instar larval stage, with Scer\GAL4^ap-md544 (Widmann and Dahmann, 2009) visible, with Scer\GAL4^Act5C.PI (Funakoshi et al., 2001) visible, with Scer\GAL4^dpp.blk1 (Tanimoto et al., 2000)
Phenotype manifest in	wing, with Scer\GAL4^dpp.blk1 (Tanimoto et al., 2000) wing, with Scer\GAL4^ptc-559.1 (Shen et al., 2008) wing, with Scer\GAL4^tub.PU (Crickmore and Mann, 2006) wing, with Scer\GAL4^tub.PU, Scer\GAL4^vg.int2.1 (Crickmore and Mann, 2006) wing \| prepupal stage, with Scer\GAL4^ptc-559.1 (Shen et al., 2008) wing disc, with Scer\GAL4^tub.PU (Crickmore and Mann, 2006) wing disc \| third instar larval stage, with Scer\GAL4^ap-md544 (Widmann and Dahmann, 2009) wing pouch, with Scer\GAL4^ptc-559.1 (Shen et al., 2008) wing sensillum, with Scer\GAL4^Act5C.PI (Funakoshi et al., 2001) wing vein, with Scer\GAL4^Act5C.PI (Funakoshi et al., 2001) wing vein L4, with Scer\GAL4^dpp.blk1 (Tanimoto et al., 2000) wing vein L5, with Scer\GAL4^dpp.blk1 (Tanimoto et al., 2000)
Other information

Allele	w^+mC
Reference(s)	(Nellen et al., 1996)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	pP{UAS-tkv.Na} (Nellen et al., 1996)
descendant(s)
Comments

Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{UAS-tkv.Na} (FlyBase, 1996-) UAS-tkv (Gibson and Perrimon, 2005, Shen and Dahmann, 2005, Funakoshi et al., 2001, Tanimoto et al., 2000, Nellen et al., 1996, Crickmore and Mann, 2006, Crickmore and Mann, 2007, Shen et al., 2008, Widmann and Dahmann, 2009) UAS-Tkv (Kruse et al., 2004) UAStkv (Terriente et al., 2008) UAS-tkv^1a1BX (Myat et al., 2005) UAS-tkv⁺ (Crickmore and Mann, 2006)
Secondary FlyBase IDs

References ( 15 )
Research paper	Widmann and Dahmann, 2009, J. Cell Sci. 122(9): 1362--1373 Dpp signaling promotes the cuboidal-to-columnar shape transition of Drosophila wing disc epithelia by regulating Rho1. [FBrf0207672] Shen et al., 2008, Mech. Dev. 125(3-4): 233--246 optomotor-blind suppresses instability at the A/P compartment boundary of the Drosophila wing. [FBrf0202782] Terriente et al., 2008, Dev. Biol. 320(1): 102--112 The Drosophila gene zfh2 is required to establish proximal-distal domains in the wing disc. [FBrf0207390] Crickmore and Mann, 2007, Development 134(2): 327--334 Hox control of morphogen mobility and organ development through regulation of glypican expression. [FBrf0194329] Crickmore and Mann, 2006, Science 313(5783): 63--68 Hox control of organ size by regulation of morphogen production and mobility. [FBrf0195181] Gibson and Perrimon, 2005, Science 307(5716): 1785--1789 Extrusion and death of DPP/BMP-compromised epithelial cells in the developing Drosophila wing. [FBrf0184250] Myat et al., 2005, Dev. Biol. 281(1): 38--52 A molecular link between FGF and Dpp signaling in branch-specific migration of the Drosophila trachea. [FBrf0187390] Reim and Frasch, 2005, Development 132(22): 4911--4925 The Dorsocross T-box genes are key components of the regulatory network controlling early cardiogenesis in Drosophila. [FBrf0190316] Shen and Dahmann, 2005, Dev. Biol. 279(1): 31--43 The role of Dpp signaling in maintaining the Drosophila anteroposterior compartment boundary. [FBrf0183859] Kruse et al., 2004, Development 131(19): 4843--4856 Dpp gradient formation by dynamin-dependent endocytosis: receptor trafficking and the diffusion model. [FBrf0180152] Funakoshi et al., 2001, Development 128(1): 67--74 mtv shapes the activity gradient of the Dpp morphogen through regulation of thickveins. [FBrf0131291] Tanimoto et al., 2000, Mol. Cell 5(1): 59--71 Hedgehog creates a gradient of DPP activity in Drosophila wing imaginal discs. [FBrf0125394] Vincent et al., 1997, Development 124(14): 2741--2750 DPP controls tracheal cell migration along the dorsoventral body axis of the Drosophila embryo. [FBrf0097693] Nellen et al., 1996, Cell 85(3): 357--368 Direct and long-range action of a DPP morphogen gradient. [FBrf0087626]
Supplementary material	Crickmore and Mann, 2006, Science 3485(5783): Supporting online material. [FBrf0199087]