Recombinant construct P{UAS-tkv.Q253D.Nb}

General Information
Symbol	P{UAS-tkv.Q253D.Nb}	FlyBase ID	FBtp0001199
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	1 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Nellen et al., 1996)
Uses	CV term Qualifiers & info Reference binary system (targeted) tkv (Nellen et al., 1996) characterization tkv (Nellen et al., 1996)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference binary component (Nellen et al., 1996) chimeric gene (Nellen et al., 1996) modified gene (Nellen et al., 1996) selectable marker (Nellen et al., 1996)
Component Alleles
Allele	tkv^{Q253D.Scer\UAS.cNb}
Reference(s)	(Crickmore and Mann, 2007, Marty et al., 2001, Bessa and Casares, 2005, Fujise et al., 2003, Allan et al., 2003, Pena-Rangel et al., 2002, Rangarajan et al., 2001, Nakagoshi et al., 2002, Cavodeassi et al., 2002, de Navas et al., 2006, Gorfinkiel et al., 2003, Hemphala et al., 2003, Kwon et al., 2004, Bennett and Alphey, 2002, Bessa et al., 2002, Bharathi et al., 2004, Moreno and Basler, 2004, Cordero et al., 2007, Tanimoto et al., 2000, Fernandez et al., 2000, Tomoyasu et al., 2000, Steneberg et al., 1999, Fuss and Hoch, 1998, Henderson et al., 1999, Garoia et al., 2005, Kuhnlein et al., 1997, Tsuneizumi et al., 1997, Riesgo-Escovar and Hafen, 1997, Schulz et al., 2004, Brugger et al., 2004, Morel and Arias, 2004, Martin et al., 2004, Keisman and Baker, 2001, Lee and Treisman, 2001, Su et al., 1998, Nellen et al., 1996, Hazelett et al., 1998, Arquier et al., 2001, Shivdasani and Ingham, 2003, Yin and Frasch, 1998, Vincent et al., 1997, Riesgo-Escovar and Hafen, 1997, Glise and Noselli, 1997, Estrada et al., 2006, Crickmore and Mann, 2006, Crickmore and Mann, 2006, Ramel et al., 2007, Letizia et al., 2007, Wada et al., 2007, Rodriguez, 2011, Shen et al., 2008, Zeng et al., 2007, Yan et al., 2009, Schlichting and Dahmann, 2008, Brás-Pereira and Casares, 2008, Fontenele et al., 2009, Barrio et al., 2007, Widmann and Dahmann, 2009, Vuilleumier et al., 2010, Ninov et al., 2010, Higashi-Kovtun et al., 2010, Wang and Ward, 2010, Szuperák et al., 2011, Khuong et al., 2010)
Molecular data	Encodes a constitutively active form of tkv. (Kuhnlein et al., 1997) Construct: Amino acid replacement: Q253D. (Nellen et al., 1996)
Phenotypic class	increased cell death, with Scer\GAL4^{nos.UTR.T:Hsim\VP16} (Schulz et al., 2004) visible, with Scer\GAL4^cad-em459 (Gorfinkiel et al., 2003) visible, with Scer\GAL4^dpp.blk1 (Bharathi et al., 2004, Zeng et al., 2007) visible, with Scer\GAL4^GMR.PF (Ramel et al., 2007) visible, with Scer\GAL4^vg.PM (Tsuneizumi et al., 1997)
Phenotype manifest in	analia, with Scer\GAL4^cad-em459 (Gorfinkiel et al., 2003) anterior crossvein, with Scer\GAL4^dpp.blk1 (Bharathi et al., 2004) apoptotic amnioserosa (Wada et al., 2007) denticle belt, with Scer\GAL4^69B (Nellen et al., 1996) embryonic/first instar larval cuticle, with Scer\GAL4^69B (Fernandez et al., 2000) embryonic/larval dorsal branch, with Scer\GAL4^btl.PS (Steneberg et al., 1999) embryonic/larval tracheal system, with Scer\GAL4^btl.PS (Vincent et al., 1997) embryonic epidermis, with Scer\GAL4^prd.RG1 (Arquier et al., 2001) epidermal cell \| larval stage \| somatic clone, with Scer\GAL4^{Scer\FRT.Act5C} (Ninov et al., 2010) eye, with Scer\GAL4^GMR.PF (Ramel et al., 2007) genitalia, with Scer\GAL4^cad-em459 (Gorfinkiel et al., 2003) germline cell \| male, with Scer\GAL4^{nos.UTR.T:Hsim\VP16} (Schulz et al., 2004) histoblast \| somatic clone, with Scer\GAL4^{Scer\FRT.Act5C} (Ninov et al., 2010) histoblast nest \| somatic clone, with Scer\GAL4^{Scer\FRT.Act5C} (Ninov et al., 2010) hub cell, with Scer\GAL4^{nos.UTR.T:Hsim\VP16} (Schulz et al., 2004) mesoderm, with Scer\GAL4^how-24B, Scer\GAL4^twi.PG (Yin and Frasch, 1998) ommatidium, with Scer\GAL4^GMR.PF (Ramel et al., 2007) pericardial cell, with Scer\GAL4^how-24B, Scer\GAL4^twi.PG (Yin and Frasch, 1998) testis \| germline clone, with Scer\GAL4^Act5C.PI (Shivdasani and Ingham, 2003) wing, with Scer\GAL4^nub-AC-62 (Martin et al., 2004, Barrio et al., 2007) wing, with Scer\GAL4^salm-459.2 (Barrio et al., 2007) wing, with Scer\GAL4^vg.PM (Tsuneizumi et al., 1997) wing blade, with Scer\GAL4^dpp.blk1 (Zeng et al., 2007) wing cell, with Scer\GAL4^e22c (Yan et al., 2009) wing cell, with Scer\GAL4^hs.PB (Yan et al., 2009) wing disc, with Scer\GAL4^C-765 (Nellen et al., 1996) wing disc, with Scer\GAL4^nub-AC-62 (Martin et al., 2004) wing disc \| anterior/posterior compartment boundary, with Scer\GAL4^dpp.blk1 (Bharathi et al., 2004) wing margin \| anterior compartment, with Scer\GAL4^vg.int2.1 (Bennett and Alphey, 2002) wing margin bristle \| anterior compartment, with Scer\GAL4^vg.int2.1 (Bennett and Alphey, 2002) wing pouch, with Scer\GAL4^dpp.blk1 (Shen et al., 2008) wing vein, with Scer\GAL4^e22c (Yan et al., 2009) wing vein, with Scer\GAL4^hs.PB (Yan et al., 2009) wing vein, with Scer\GAL4^nub-AC-62 (Barrio et al., 2007) wing vein, with Scer\GAL4^salm-459.2 (Barrio et al., 2007) wing vein L5, with Scer\GAL4^vg.int2.1 (Bennett and Alphey, 2002)
Other information

Allele	w^+mC
Reference(s)	(Nellen et al., 1996)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	pP{UAS-tkv.Q253D.Nb} (Nellen et al., 1996)
descendant(s)
Comments

Stocks Listed in FlyBase ( 1 )
Bloomington	36536 w^*; P{UAS-tkv.Q253D.Nb}3/TM3, Sb¹ Ser¹
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{UAS-tkv.Q253D.Nb} (FlyBase, 1996-) TKV^act (Khuong et al., 2010) tkv^Q253D (Yan et al., 2009) tkv^QD (Vuilleumier et al., 2010) UAS-tkv (Gorfinkiel et al., 2003) UAS-tkv.QD} (Bessa and Casares, 2005) UAS-tkv* (Schulz et al., 2004, Shivdasani and Ingham, 2003, Keisman and Baker, 2001, Tomoyasu et al., 2000, Tanimoto et al., 2000) UAS-Tkv* (Fernandez et al., 2000, Bharathi et al., 2004) UAS-tkv^A (Allan et al., 2003, Allan et al., 2003) UAS-tkv^act (Rangarajan et al., 2001) UAStkv^act (Khuong et al., 2010) UAS-tkv^DN (Garoia et al., 2005, Fontenele et al., 2009) UAS-tkv^Q253D (Kwon et al., 2004, Fujise et al., 2003, Nakagoshi et al., 2002, Bennett and Alphey, 2002, Arquier et al., 2001, Steneberg et al., 1999, Nellen et al., 1996, Henderson et al., 1999, de Navas et al., 2006, Wada et al., 2007, Schlichting and Dahmann, 2008, Higashi-Kovtun et al., 2010, Wang and Ward, 2010) UAS Tkv^Q253D (Morel and Arias, 2004) UAS-tkv^QD (Martin et al., 2004, Moreno and Basler, 2004, Cavodeassi et al., 2002, Lee and Treisman, 2001, Estrada et al., 2006, Ramel et al., 2007, Szuperák et al., 2011, Letizia et al., 2007, Shen et al., 2008, Zeng et al., 2007, Yan et al., 2009, Brás-Pereira and Casares, 2008, Barrio et al., 2007) UAS-Tkv^QD (Bessa et al., 2002, Fuss and Hoch, 1998, Ninov et al., 2010) UAS-tkv^Q-D (Widmann and Dahmann, 2009) UAS-tkvQ253D (Hemphala et al., 2003, Cordero et al., 2007) UAS-tkvQD (Marty et al., 2001, Crickmore and Mann, 2006, Crickmore and Mann, 2007) UAS TkvQD (Rodriguez, 2011)
Secondary FlyBase IDs

References ( 66 )

Generate a list of	All references relating to this transgenic transposon ( 66 )

List References by type	Research paper ( 63 ) Supplementary material ( 2 ) Personal communication to FlyBase ( 1 )
Recent research papers ( 2 )
	Rodriguez, 2011, PLoS ONE 6(4): e18418 Drosophila TIEG Is a Modulator of Different Signalling Pathways Involved in Wing Patterning and Cell Proliferation. [FBrf0213463] Szuperák et al., 2011, Development 138(4): 715--724 Feedback regulation of Drosophila BMP signaling by the novel extracellular protein Larval Translucida. [FBrf0212881] Show all research papers ...