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General Information
Symbol
P{UAS-GFP.nls}
FlyBase ID
FBtp0001204
Feature type
transgenic_transposon
Component Allele(s)
Also Known As
UAS-GFP.nls, UAS-GFP, UAS-GFPnls, UAS-nls-GFP, UAS-nlsGFP, UAS-GFPnls, UAS-nGFP, UAS-GFP-NLS, GFP-NLS, UAS-nls::GFP
Size
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Location-dependent role
Description
CV term
Qualifiers and info
Reference
transposon
Construct components
Product class / Tool use(s)
Marker(s)
Product class / Tool use(s)
Encoded product / tool
Description

Expression of GFP, tagged at its N terminal end with the 15 amino acid SV40 nuclear localization signal, is governed by UAS regulatory sequences.

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Features
CV term
Qualifiers and info
Reference
binary
component
chimeric
gene
molecular
tag
reporter
gene
selectable
marker
Component Alleles
Reference(s)
(Mehta et al., 2020, Andreyeva et al., 2019, Guo et al., 2019, Morón-Oset et al., 2019, Phan et al., 2019, Conway et al., 2018, Pu et al., 2018, Perry et al., 2015, Azuma et al., 2014, Harrop et al., 2014, Hauling et al., 2014, Lawton et al., 2014, Martín-Peña et al., 2014, Eun et al., 2013, Hopkins, 2013, Ibrahim et al., 2013, Jepson et al., 2013, Lindström et al., 2013, Neisch et al., 2013, Shen et al., 2013, Uchino et al., 2013, Dahlgaard et al., 2012, Denton et al., 2012, Edwards et al., 2012, Kennell et al., 2012, Saleem et al., 2012, Unhavaithaya and Orr-Weaver, 2012, Weiss et al., 2012, Acebes et al., 2011, Benchabane et al., 2011, Benchabane et al., 2011, Cáceres et al., 2011, Cho and Fischer, 2011, Estes et al., 2011, Gallio et al., 2011, Hasegawa et al., 2011, Keller et al., 2011, Lee et al., 2011, Li et al., 2011, Vrailas-Mortimer et al., 2011, Wang et al., 2011, Alekseyenko et al., 2010, Ballard et al., 2010, Lam et al., 2010, Zhang et al., 2010, Guha et al., 2009, Issigonis et al., 2009, Jacobsson et al., 2009, Jansen et al., 2009, Mitri et al., 2009, Mohseni et al., 2009, Mosimann et al., 2009, Shahidullah et al., 2009, Awasaki et al., 2008, Chang et al., 2008, Chen et al., 2008, Costantino et al., 2008, Hong et al., 2008, Lee et al., 2008, Thorne and Amrein, 2008, Watson et al., 2008, Wu et al., 2008, Caldwell et al., 2007, Duan et al., 2007, Kiger et al., 2007, Wada et al., 2007, Wang et al., 2007, Wang et al., 2007, Baena-Lopez and Garcia-Bellido, 2006, Le Bras and Van Doren, 2006, Maqbool et al., 2006, Mosimann et al., 2006, Muro et al., 2006, Palanker et al., 2006, Sato et al., 2006, Umetsu et al., 2006, Yasunaga et al., 2006, Araki et al., 2005, Caldwell et al., 2005, Caldwell et al., 2005, Chen et al., 2005, Cole et al., 2005, Guha and Kornberg, 2005, McClure and Schubiger, 2005, Monier et al., 2005, Ralston and Blair, 2005, Rawson, 2005, Rawson et al., 2005, Xu et al., 2005, Yoshida et al., 2005, Yuan et al., 2005, Dominguez et al., 2004, Dutta et al., 2004, Galko and Krasnow, 2004, Kimura et al., 2004, Soler et al., 2004, Thorne et al., 2004, Araki et al., 2003, Bidet et al., 2003, Cherbas et al., 2003, Fichelson and Gho, 2003, Kenyon et al., 2003, Labrador and Corces, 2003, Marygold and Vincent, 2003, Mergliano and Minden, 2003, Perrin et al., 2003, Robertson et al., 2003, Roignant et al., 2003, Sen et al., 2003, Ahmad and Baker, 2002, Behan et al., 2002, Held, 2002, Rulifson et al., 2002, Williams and Shepherd, 2002, Dong et al., 2001, Keisman et al., 2001, Loren et al., 2001, Pfeiffer et al., 2000, Gho et al., 1999, Jarecki et al., 1999, Weinkove et al., 1999, Shiga et al., 1996)
Molecular data

Expression of GFP, tagged at its N terminal end with the 15 amino acid SV40 nuclear localization signal, is governed by UAS regulatory sequences.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
descendant(s)
Comments

report comment: Sequences for SV40 nuclear localization signal fused at 5\' end of GFP sequences.

construct_comment: Designed as vital cell marker, however levels of fluorescence very low in diploid cells.

Stocks (6)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (52)
Reported As
Symbol Synonym
P {w [+mC]= UAS-GFP. nls}
PUAS-GFP.nls
Pw<up>+;UAS-GFP nls</up>
P{UAS-GFP.N}
UAS-GFP-nls14
UAS-gfp-nls
UAS-gfp.nls
UAS-nls-EGFP
UAS-nucgfp8
UAS-nuclear GFP
UAS-nuclear-localization-signal-GFP
UAS-nuclearGFP
Secondary FlyBase IDs
    References (126)