Recombinant construct P{UAS-y.C}

General Information
Symbol	P{UAS-y.C}	FlyBase ID	FBtp0001209
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	2 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Calleja, 1996.10.16)
Uses	CV term Qualifiers & info Reference binary system (targeted) y (Calleja, 1996.10.16) characterization y (Calleja, 1996.10.16) reporter construct y \| binary system (Calleja et al., 1996)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference binary component (Calleja, 1996.10.16) chimeric gene (Calleja, 1996.10.16) selectable marker (Calleja, 1996.10.16, Calleja et al., 1996)
Component Alleles
Allele	w^+mC
Reference(s)	(Calleja, 1996.10.16, Calleja et al., 1996)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information

Allele	y^Scer\UAS.cCa
Reference(s)	(Calleja, 1996.10.16, Jeong et al., 2008, Giordano et al., 2002, Drapeau et al., 2003, Wang and Struhl, 2005, Wittkopp et al., 2002, Gorfinkiel et al., 1999, Calleja et al., 2000, Azpiazu and Morata, 2000, Sivasankaran et al., 2000, Gorfinkiel et al., 1997, Heitzler et al., 1996, Lecuit et al., 1996, Calleja et al., 1996, Suzanne et al., 2003, Pastor-Pareja et al., 2004, de Navas et al., 2006, Riedel et al., 2011, de Navascués and Modolell, 2010)
Molecular data	Expression of a 2kb y cDNA, including the entire y coding region, is governed by UAS regulatory sequences. (Calleja, 1996.10.16) A full length y cDNA has been reconstituted by ligating two EcoRI fragments and is expressed from Scer\UAS regulatory sequences. (Calleja et al., 1996) y expression is influenced by Scer\UAS Scer\GAL4 binding sites. (Heitzler et al., 1996) Construct: Expression of y⁺ is influenced by Scer\UAS Scer\GAL4 binding sites. (Lecuit et al., 1996)
Phenotypic class	body color defective, with Scer\GAL4^en-e16E (Riedel et al., 2011) body color defective, with Scer\GAL4^pnr-MD237 (Wittkopp et al., 2002)
Phenotype manifest in	adult cuticle, with Scer\GAL4^hs.PB (Calleja et al., 1996) adult cuticle, with Scer\GAL4^pnr-MD237 (Calleja et al., 1996) analia, with Scer\GAL4^cad-em459 (Calleja et al., 1996) antennal segment, with Scer\GAL4^Dll-md23 (Calleja et al., 1996) metatarsus, with Scer\GAL4^Dll-md23 (Calleja et al., 1996) pretarsus, with Scer\GAL4^Dll-md23 (Calleja et al., 1996) tarsal segment 2, with Scer\GAL4^Dll-md23 (Calleja et al., 1996) tarsal segment 3, with Scer\GAL4^Dll-md23 (Calleja et al., 1996) tarsal segment 4, with Scer\GAL4^Dll-md23 (Calleja et al., 1996) tibia, with Scer\GAL4^Dll-md23 (Calleja et al., 1996) wing, with Scer\GAL4^Dll-md23 (Calleja et al., 1996) wing blade \| posterior compartment, with Scer\GAL4^en-e16E (Riedel et al., 2011)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	pP{UAS-y.C} (Calleja, 1996.10.16)
descendant(s)
Comments
	report comment: cDNA 2kb in length; contains entire y⁺ coding sequence. (Calleja, 1996.10.16)
Stocks Listed in FlyBase ( 8 )
Bloomington	36525 P{GawB}MD758/CyO; P{UAS-y.C}MC1 3043 y¹ w¹¹¹⁸; P{UAS-y.C}MC1 36520 y¹ w^* P{GawB}fz3^MD810; P{UAS-y.C}MC1 3040 y¹ w¹¹¹⁸; P{GawB}tsh^md621/CyO; P{UAS-y.C}MC1/TM2 3039 y¹ w¹¹¹⁸; P{GawB}pnr^MD237/TM3, P{UAS-y.C}MC2, Ser¹
Kyoto	106797 y¹ w¹¹¹⁸; P{GawB}tsh^md621/CyO; P{UAS-y.C}MC1/TM2 106800 y¹ w¹¹¹⁸; P{UAS-y.C}MC1/TM3, P{UAS-y.C}MC2, Ser¹ 106796 y¹ w¹¹¹⁸; P{GawB}pnr^MD237/TM3, P{UAS-y.C}MC2, Ser¹
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{UAS-y.C} (FlyBase, 1996-) UAS-y (Suzanne et al., 2003, Drapeau et al., 2003, Calleja et al., 2000, Azpiazu and Morata, 2000, de Navascués and Modolell, 2010) UASy (Calleja, 1996.10.16) UAS-y.C (Pastor-Pareja et al., 2004) UAS-y⁺ (Wang and Struhl, 2005, Giordano et al., 2002, Gorfinkiel et al., 1999, de Navas et al., 2006) UAS-yellow (Wittkopp et al., 2002, Riedel et al., 2011, Jeong et al., 2008)
Secondary FlyBase IDs

References ( 19 )
Research paper	Riedel et al., 2011, Development 138(1): 149--158 Megalin-dependent Yellow endocytosis restricts melanization in the Drosophila cuticle. [FBrf0212536] de Navascués and Modolell, 2010, Mech. Dev. 127(9-12): 393--406 The pronotum LIM-HD gene tailup is both a positive and a negative regulator of the proneural genes achaete and scute of Drosophila. [FBrf0212072] Jeong et al., 2008, Cell 132(5): 783--793 The evolution of gene regulation underlies a morphological difference between two Drosophila sister species. [FBrf0203102] de Navas et al., 2006, Mech. Dev. 123(11): 860--867 A simple and efficient method to identify replacements of P-lacZ by P-Gal4 lines allows obtaining Gal4 insertions in the bithorax complex of Drosophila. [FBrf0194816] Wang and Struhl, 2005, Development 132(12): 2883--2894 Distinct roles for Mind bomb, Neuralized and Epsin in mediating DSL endocytosis and signaling in Drosophila. [FBrf0187489] Pastor-Pareja et al., 2004, Dev. Cell 7(3): 387--399 Invasive cell behavior during Drosophila imaginal disc eversion is mediated by the JNK signaling cascade. [FBrf0180122] Drapeau et al., 2003, J. Neurobiol. 55(1): 53--72 A gene necessary for normal male courtship, yellow, acts downstream of fruitless in the Drosophila melanogaster larval brain. [FBrf0159053] Suzanne et al., 2003, Dev. Biol. 260(2): 465--483 The hernandez and fernandez genes of Drosophila specify eye and antenna. [FBrf0161494] Giordano et al., 2002, Genetics 160(2): 637--648 RNAi triggered by symmetrically transcribed transgenes in Drosophila melanogaster. [FBrf0144904] Wittkopp et al., 2002, Development 129(8): 1849--1858 Reciprocal functions of the Drosophila yellow and ebony proteins in the development and evolution of pigment patterns. [FBrf0146983] Azpiazu and Morata, 2000, Development 127(12): 2685--2693 Function and regulation of homothorax in the wing imaginal disc of Drosophila. [FBrf0128381] Calleja et al., 2000, Development 127(18): 3971--3980 Generation of medial and lateral dorsal body domains by the pannier gene of Drosophila. [FBrf0129751] Sivasankaran et al., 2000, Mech. Dev. 91(1-2): 427--431 The wingless target gene dfz3 encodes a new member of the Drosophila frizzled family. [FBrf0127331] Gorfinkiel et al., 1999, Mech. Dev. 86(1-2): 113--123 Drosophila terminalia as an appendage-like structure. [FBrf0111374] Gorfinkiel et al., 1997, Genes Dev. 11(17): 2259--2271 The homeobox gene Distal-less induces ventral appendage development in Drosophila. [FBrf0098249] Calleja et al., 1996, Science 274(5285): 252--255 Visualization of gene expression in living adult Drosophila. [FBrf0090484] Heitzler et al., 1996, Genetics 143(3): 1271--1286 A genetic analysis of pannier, a gene necessary for viability of dorsal tissues and bristle positioning in Drosophila. [FBrf0088194] Lecuit et al., 1996, Nature 381(6581): 387--393 Two distinct mechanisms for long-range patterning by decapentaplegic in the Drosophila wing. [FBrf0087557]
Personal communication to FlyBase	Calleja, 1996.10.16, [title not yet available] [title not yet available] [FBrf0089316]