Recombinant construct P{mc/DTA-D}

General Information
Symbol	P{mc/DTA-D}	FlyBase ID	FBtp0001280
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	0 available
Molecular map
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Park and Wolfner, 1995)
Uses	CV term Qualifiers & info Reference analytical tool cell ablation (Park and Wolfner, 1995)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference selectable marker (Kalb et al., 1993, Chapman et al., 1995)
Component Alleles
Allele	Cbβ\DT-A^{E148D.Acp95EF}
Reference(s)	(Kalb et al., 1993, Chapman et al., 1995, Tram and Wolfner, 1999, Park and Wolfner, 1995)
Molecular data	Construct: Amino acid replacement: E148D. Wild type 400bp AccI-PscI fragment of Cbβ\DT-A^Acp95EF.PK is substituted with the mutated fragment. (Kalb et al., 1993)
Phenotypic class	male fertile \| poor (Tram and Wolfner, 1999)
Phenotype manifest in	female accessory gland main cell (Kalb et al., 1993)
Other information

Allele	w^+mW.hs
Reference(s)	(Kalb et al., 1993, Chapman et al., 1995)
Molecular data	A mini-w gene is expressed under the control of an Hsp70 promoter. (Heinrich et al., 1993) w gene expression is under the influence of FLIP recombinase. (Golic and Lindquist, 1989) Expression of a fragment of w genomic DNA with a 2.2kb deletion of 3' flanking sequences and a reduction in the size of the largest intron to 356bp is driven by a 388bp fragment of an Hsp70 gene. (Klemenz et al., 1987) Construct: A 'mini-white' gene constructed by deletion of the XbaI-HindII fragment from the long 5' intron. Carried by the W6 and W8 series of vectors.
Phenotypic class	suppressor of variegation, with Su(var)205⁵⁰² (Ahmad and Golic, 1996)
Phenotype manifest in	pigment cell (Kurkulos et al., 1994, Golic and Lindquist, 1989)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	DTA-D (Tram and Wolfner, 1999) P{mc/DTA-D} (FlyBase, 1996-)
Secondary FlyBase IDs

References ( 4 )
Research paper	Tram and Wolfner, 1999, Genetics 153(2): 837--844 Male seminal fluid proteins are essential for sperm storage in Drosophila melanogaster. [FBrf0111505] Chapman et al., 1995, Nature 373(6511): 241--244 Cost of mating in Drosophila melanogaster females is mediated by male accessory gland products. [FBrf0079936] Park and Wolfner, 1995, Dev. Biol. 171(2): 694--702 Male and female cooperate in the prohormone-like processing of a Drosophila melanogaster seminal fluid protein. [FBrf0084252] Kalb et al., 1993, Proc. Natl. Acad. Sci. U.S.A. 90(17): 8093--8097 Probing the function of Drosophila melanogaster accessory glands by directed cell ablation. [FBrf0059294]