UASt regulatory sequences drive expression of a constitutively activated, ligand-independent form of N. The signal sequence and signal cleavage site of Dl (amino acid residues 1-27) are fused to N sequence from amino acid 1742 to the end of the coding sequence. A frameshift mutation in the N coding sequence causes premature termination after amino acid residue 2235 with 4 residues (RPPT) appended to the C terminus.
construct_comment: Obtained from E. Giniger.
report comment: Notch protein produced is constitutively active.