Recombinant construct P{EP}

General Information
Symbol	P{EP}	FlyBase ID	FBtp0001317
Feature type	transgenic_transposon

Size	7.987	Expression data
Associated insertions	3747 available
Molecular map
Recent Updates
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FB2012_01	Insertions P{EP}puc[EP3194] P{EP}GE10558 P{EP}mgl[GE3293] P{EP}l(3)S055409[S055409] References Lo et al., 2007, Dev. Biol. 311(2): 287--296 Tripura et al., 2011, Int. J. Dev. Biol. 55(6): 583--590 Link et al., 2007, J. Cell Biol. 178(4): 567--574 Riedel et al., 2011, Development 138(1): 149--158 Alleles puc[EP3194] Hand[173] msn[S055409] mgl[GE3293] Sip1[EP2037]
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Description & Uses
Species	synthetic construct
Location-dependent role	mobile activating element (UASG) (Rorth, 1996)
Description	CV term Qualifiers & info Reference transposon P-element (Rorth, 1996, Rorth, 1998.4.4, Parks et al., 2004, Bellen et al., 2004)
Uses	CV term Qualifiers & info Reference genomic insertion screen mobile activating element UAS (Rorth, 1996)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)	FlyBase-compiled
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size	7.987
Left end	P5\' (Rorth, 1996)
Right end	P3\' (Rorth, 1996)

Segments	Number Orientation Symbol 1 + CaSpeR_P5'(P5',Sp) 2 + 001268 3 - pHSS7(N,R1)b 4 + NotI 5 + GAGA_sites 6 + 001271 7 + 14xUAS_Ghs 8 + 001269 9 + Car1_P3'(H3,P3')
Reference	(Rorth, 1998.4.4)
Features
	CV term Qualifiers & info Reference bacterial selection (Mlodzik and Hiromi, 1992) mobile activating (Rorth, 1996, Rorth, 1998.4.4) partial rescue (Pirrotta, 1988) regulatory/structural DNA (Rorth, 1996) selectable marker (Pirrotta, 1988)
Component Alleles
Allele	Scer\UAS^14x.hs
Reference(s)	(Rorth, 1996, Rorth, 1998.4.4)
Molecular data	Construct: 14 copies of Scer\GAL4 binding sites, followed by a fragment of the Hsp70 promoter from -45 to +33. (Rorth, 1996)
Phenotypic class
Phenotype manifest in
Other information

Allele	TrlBR^cRa
Reference(s)	(Rorth, 1996)
Molecular data	Construct: Contains two copies of a 'GAGA element' which can bind Trl. (Rorth, 1996)
Phenotypic class
Phenotype manifest in
Other information

Allele	w^+mC
Reference(s)	(Pirrotta, 1988, Rorth, 1996)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Assay mode
Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	pP{EP} (Rorth, 1998.4.4)
descendant(s)	P{3'EP} (FlyBase, 1992-) P{5'EP} (FlyBase, 1992-) P{?'EP} (FlyBase, 1992-) P{?EP} (FlyBase, 1992-, Park et al., 2006) P{EP-Δ9} (Van Buskirk and Schupbach, 2002) P{EP-Δ10} (Sullivan et al., 2001) P{EP-Δ13} (Van Buskirk and Schupbach, 2002) P{EP-Δ15} (Sullivan et al., 2001) P{EP-Δ19} (Sullivan et al., 2001) P{EP-Δ191} (Plank et al., 2005) P{EP-ΔA10} (Guichet et al., 2002)
Comments
	construct_comment: Used for modular misexpression screen.GAL4-activated transcription from Hsp70 promoter is toward P3\', extending into adjacent genomic sequences.Restriction site useful for plasmid rescue: EcoRI. (Rorth, 1996)
Stocks Listed in FlyBase ( 1769 )
Bloomington	26582 w^* P{EP}G74 33439 w^* P{EP}G59 33441 w^* P{EP}G78 33514 P{EP}G64 w^* 33516 w^* P{EP}G91 26583 w^* P{EP}G249 26585 w^* P{EP}G307 26586 w^* P{EP}G313 26588 w^* P{EP}G327 26590 P{EP}G352 w^* 26593 w^* P{EP}G400 26594 w^* P{EP}G524
	More stocks available...
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{EP} (FlyBase, 1996-, Parks et al., 2004, Bellen et al., 2004, BDGP Project Members, 1994-1999) P{Mae-UAS.EP} (FlyBase, 1996-)
Secondary FlyBase IDs

References ( 151 )

Generate a list of	All references relating to this transgenic transposon ( 151 )

List References by type	Research paper ( 115 ) Supplementary material ( 9 ) Review ( 1 ) Personal communication to FlyBase ( 20 ) FlyBase analysis ( 4 ) Computer file ( 2 )
Recent research papers ( 21 )
	Cachero et al., 2011, PLoS Biol. 9(1): e1000568 The gene regulatory cascade linking proneural specification with differentiation in Drosophila sensory neurons. [FBrf0212891] Ding et al., 2011, Dev. Dyn. 240(1): 122--134 Glial cell adhesive molecule unzipped mediates axon guidance in Drosophila. [FBrf0212620] Freeman et al., 2011, Mol. Cell. Neurosci. 46(2): 535--547 NFAT regulates pre-synaptic development and activity-dependent plasticity in Drosophila. [FBrf0212894] Lim et al., 2011, Nature 470(7334): 399--403 The novel gene twenty-four defines a critical translational step in the Drosophila clock. [FBrf0213059] Morawe et al., 2011, J. Cell Biol. 193(1): 71--80 Loss of the extraproteasomal ubiquitin receptor Rings lost impairs ring canal growth in Drosophila oogenesis. [FBrf0213374] Riedel et al., 2011, Development 138(1): 149--158 Megalin-dependent Yellow endocytosis restricts melanization in the Drosophila cuticle. [FBrf0212536] Suissa et al., 2011, J. Biol. Chem. 286(17): 15556--15564 The NAB-Brk Signal Bifurcates at JNK to Independently Induce Apoptosis and Compensatory Proliferation. [FBrf0213516] Tripura et al., 2011, Int. J. Dev. Biol. 55(6): 583--590 Regulation and activity of JNK signaling in the wing disc peripodial membrane during adult morphogenesis in Drosophila. [FBrf0216296] Blanchard et al., 2010, J. Neurosci. 30(17): 5855--5865 The transcription factor Mef2 is required for normal circadian behavior in Drosophila. [FBrf0210652] Cully et al., 2010, Mol. Cell. Biol. 30(2): 481--495 A role for p38 stress-activated protein kinase in regulation of cell growth via TORC1. [FBrf0209636] Fan et al., 2010, Genetics 184(1): 141--154 Drosophila translational elongation factor-1{gamma} is modified in response to DOA kinase activity and is essential for cellular viability. [FBrf0209673] Houot et al., 2010, Genetics 185(4): 1297--1309 The Consequences of Regulation of desat1 Expression for Pheromone Emission and Detection in Drosophila melanogaster. [FBrf0211531] Kim et al., 2010, Dev. Biol. 346(2): 247--257 Drosophila Cand1 regulates Cullin3-dependent E3 ligases by affecting the neddylation of Cullin3 and by controlling the stability of Cullin3 and adaptor protein. [FBrf0211875] Kim et al., 2010, Biochem. Biophys. Res. Commun. 402(3): 565--570 dGIPC is required for the locomotive activity and longevity in Drosophila. [FBrf0212394] Mottola et al., 2010, Development 137(14): 2353--2364 A novel function for the Rab5 effector Rabenosyn-5 in planar cell polarity. [FBrf0211104] Müller et al., 2010, PLoS ONE 5(12): e14323 Regulation and Functions of the lms Homeobox Gene during Development of Embryonic Lateral Transverse Muscles and Direct Flight Muscles in Drosophila. [FBrf0212632] Park et al., 2010, PLoS ONE 5(10): e13151 Drosophila Porin/VDAC Affects Mitochondrial Morphology. [FBrf0212060] Schneiderman et al., 2010, PLoS Genet. 6(9): e1001095 Perturbation Analysis of Heterochromatin-Mediated Gene Silencing and Somatic Inheritance. [FBrf0211786] Song et al., 2010, Genes Dev. 24(9): 881--886 Coop functions as a corepressor of Pangolin and antagonizes Wingless signaling. [FBrf0213249] Sun et al., 2010, Nat. Struct. Mol. Biol. 17(10): 1188--1194 A metazoan ortholog of SpoT hydrolyzes ppGpp and functions in starvation responses. [FBrf0212027] van der Knaap et al., 2010, Mol. Cell. Biol. 30(3): 736--744 Biosynthetic enzyme GMP synthetase cooperates with ubiquitin-specific protease 7 in transcriptional regulation of ecdysteroid target genes. [FBrf0209736] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...