Recombinant construct P{EP}

General Information
Symbol	P{EP}	FlyBase ID	FBtp0001317
Feature type	transgenic_transposon

Size	7.987	Expression data
Associated insertions	3862 available
Molecular map
Recent Updates
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FB2013_03	Insertions P{EP}mbl[B2-E1] P{EP}Ubp64E[E213-1A] P{EP}CG5009[B227.2] P{EP}mrj[E1050] P{EP}bun[M15] P{EP}bun[G6132] P{EP}Hsp68[E407] P{EP}wech[JM265] P{EP}bun[G12085] P{EP}wech[E546] P{EP}emb[E2-1A] P{EP}wech[JM120] P{EP}Faf[E659] P{EP}emb[E128-1A] P{EP}CG8209[B3-Sa] P{EP}Tpr2[EB7-1A] P{EP}orb2[B8-S] P{EP}DnaJ-1[B345.2] P{EP}bun[G12937] P{EP}jub[EPG845] Alleles bun[M15] wech[E546] bun[G12085] wech[JM120] DnaJ-1[B345.2] Tpr2[EB7-1A] orb2[B8-S] emb[E128-1A] CG5009[B227.2] mbl[B2-E1] mrj[E1050] Ubp64E[E213-1A] bun[G6132] Faf[E659] emb[E2-1A] jub[EPG845] CG8209[B3-Sa] wech[JM265] bun[G12937] Hsp68[E407] Kdm2[EP3093]
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Description & Uses
Species	synthetic construct
Location-dependent role	mobile activating element (UASG) (Rorth, 1996)
Description	CV term Qualifiers & info Reference transposon P-element (Rorth, 1996, Rorth, 1998.4.4, Parks et al., 2004, Bellen et al., 2004)
Uses	CV term Qualifiers & info Reference genomic insertion screen mobile activating element UAS (Rorth, 1996)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)	FlyBase-compiled
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size	7.987
Left end	P5\' (Rorth, 1996)
Right end	P3\' (Rorth, 1996)

Segments	Number Orientation Symbol 1 + CaSpeR_P5'(P5',Sp) 2 + 001268 3 - pHSS7(N,R1)b 4 + NotI 5 + GAGA_sites 6 + 001271 7 + 14xUAS_Ghs 8 + 001269 9 + Car1_P3'(H3,P3')
Reference	(Rorth, 1998.4.4)
Features
	CV term Qualifiers & info Reference bacterial selection (Mlodzik and Hiromi, 1992) mobile activating (Rorth, 1996, Rorth, 1998.4.4) partial rescue (Pirrotta, 1988) regulatory/structural DNA (Rorth, 1996) selectable marker (Pirrotta, 1988)
Component Alleles
Allele	Scer\UAS^14x.hs
Reference(s)	(Rorth, 1996, Rorth, 1998.4.4)
Molecular data	Construct: 14 copies of Scer\GAL4 binding sites, followed by a fragment of the Hsp70 promoter from -45 to +33. (Rorth, 1996)
Phenotypic class
Phenotype manifest in
Other information

Allele	TrlBR^cRa
Reference(s)	(Rorth, 1996)
Molecular data	Construct: Contains two copies of a 'GAGA element' which can bind Trl. (Rorth, 1996)
Phenotypic class
Phenotype manifest in
Other information

Allele	w^+mC
Reference(s)	(Pirrotta, 1988, Rorth, 1996)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	pP{EP} (Rorth, 1998.4.4)
descendant(s)	P{3'EP} (FlyBase, 1992-) P{5'EP} (FlyBase, 1992-) P{?'EP} (FlyBase, 1992-) P{?EP} (FlyBase, 1992-, Park et al., 2006) P{EP-Δ9} (Van Buskirk and Schupbach, 2002) P{EP-Δ10} (Sullivan et al., 2001) P{EP-Δ13} (Van Buskirk and Schupbach, 2002) P{EP-Δ15} (Sullivan et al., 2001) P{EP-Δ19} (Sullivan et al., 2001) P{EP-Δ191} (Plank et al., 2005) P{EP-ΔA10} (Guichet et al., 2002) P{EP-ΔC3} (Kanakousaki and Gibson, 2012) P{EP-ΔN2} (Kanakousaki and Gibson, 2012)
Comments
	Additional information at: GDP Transposons http://flypush.imgen.bcm.tmc.edu/pscreen/transposons.html (Bellen et al., 2004) construct_comment: Used for modular misexpression screen.GAL4-activated transcription from Hsp70 promoter is toward P3\', extending into adjacent genomic sequences.Restriction site useful for plasmid rescue: EcoRI. (Rorth, 1996)
Stocks Listed in FlyBase ( 1776 )
Bloomington	26582 w^* P{EP}G74 33439 w^* P{EP}G59 33441 w^* P{EP}G78 33514 P{EP}G64 w^* 33516 w^* P{EP}G91 26583 w^* P{EP}G249 26585 w^* P{EP}G307 26586 w^* P{EP}G313 26588 w^* P{EP}G327 26590 P{EP}G352 w^* 26593 w^* P{EP}G400 26594 w^* P{EP}G524
	More stocks available...
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{EP} (FlyBase, 1996-, Parks et al., 2004, Bellen et al., 2004, BDGP Project Members, 1994-1999) P{Mae-UAS.EP} (FlyBase, 1996-)
Secondary FlyBase IDs

References ( 154 )

Generate a list of	All references relating to this transgenic transposon ( 154 )

List References by type	Research paper ( 130 ) Supplementary material ( 10 ) Personal communication to FlyBase ( 11 ) FlyBase analysis ( 3 )
Recent research papers ( 24 )
	Burgess et al., 2012, Development 139(16): 3040--3050 Type II phosphatidylinositol 4-kinase regulates trafficking of secretory granule proteins in Drosophila. [FBrf0219013] Kanakousaki and Gibson, 2012, Development 139(15): 2751--2762 A differential requirement for SUMOylation in proliferating and non-proliferating cells during Drosophila development. [FBrf0218825] Kim et al., 2012, J. Biol. Chem. 287(9): 6628--6641 Glutathione s-transferase omega 1 activity is sufficient to suppress neurodegeneration in a Drosophila model of Parkinson disease. [FBrf0217543] Lee et al., 2012, J. Neurosci. 32(4): 1429--1435 Gustatory receptors required for avoiding the insecticide L-canavanine. [FBrf0217317] Lee et al., 2012, DNA Repair 11(9): 741--752 Drosophila Claspin is required for the G2 arrest that is induced by DNA replication stress but not by DNA double-strand breaks. [FBrf0219169] Luo and Sehgal, 2012, Cell 148(4): 765--779 Regulation of Circadian Behavioral Output via a MicroRNA-JAK/STAT Circuit. [FBrf0217497] Maeng et al., 2012, Mol. Cells 33(5): 457--463 The BTB/POZ-ZF transcription factor dPLZF is involved in Ras/ERK signaling during Drosophila wing development. [FBrf0218378] Nam et al., 2012, EMBO J. 31(5): 1253--1265 Genetic evidence of a redox-dependent systemic wound response via Hayan Protease-Phenoloxidase system in Drosophila. [FBrf0217593] Paik et al., 2012, Mech. Ageing Dev. 133(5): 234--245 Misexpression screen delineates novel genes controlling Drosophila lifespan. [FBrf0218389] Palm et al., 2012, PLoS Genet. 8(7): e1002828 Lipoproteins in Drosophila melanogaster-Assembly, Function, and Influence on Tissue Lipid Composition. [FBrf0219024] Tsai et al., 2012, Proc. Natl. Acad. Sci. U.S.A. 109(43): 17699--17704 Activity-dependent retrograde laminin A signaling regulates synapse growth at Drosophila neuromuscular junctions. [FBrf0219768] Cachero et al., 2011, PLoS Biol. 9(1): e1000568 The gene regulatory cascade linking proneural specification with differentiation in Drosophila sensory neurons. [FBrf0212891] Ding et al., 2011, Dev. Dyn. 240(1): 122--134 Glial cell adhesive molecule unzipped mediates axon guidance in Drosophila. [FBrf0212620] Freeman et al., 2011, Mol. Cell. Neurosci. 46(2): 535--547 NFAT regulates pre-synaptic development and activity-dependent plasticity in Drosophila. [FBrf0212894] Jung et al., 2011, FEBS Lett. 585(12): 1973--1978 Identification of Drosophila SOD3 and its protective role against phototoxic damage to cells. [FBrf0214281] Lee et al., 2011, Mol. Cells 32(5): 477--482 The phosphoinositide phosphatase Sac1 is required for midline axon guidance. [FBrf0216733] Lim et al., 2011, Nature 470(7334): 399--403 The novel gene twenty-four defines a critical translational step in the Drosophila clock. [FBrf0213059] Morawe et al., 2011, J. Cell Biol. 193(1): 71--80 Loss of the extraproteasomal ubiquitin receptor Rings lost impairs ring canal growth in Drosophila oogenesis. [FBrf0213374] Riedel et al., 2011, Development 138(1): 149--158 Megalin-dependent Yellow endocytosis restricts melanization in the Drosophila cuticle. [FBrf0212536] Rodriguez, 2011, PLoS ONE 6(4): e18418 Drosophila TIEG Is a Modulator of Different Signalling Pathways Involved in Wing Patterning and Cell Proliferation. [FBrf0213463] Sabino et al., 2011, J. Cell Sci. 124(7): 1156--1166 Drosophila Ajuba is not an Aurora-A activator but is required to maintain Aurora-A at the centrosome. [FBrf0213223] Seisenbacher et al., 2011, PLoS Genet. 7(8): e1002168 MK2-Dependent p38b Signalling Protects Drosophila Hindgut Enterocytes against JNK-Induced Apoptosis under Chronic Stress. [FBrf0214607] Suissa et al., 2011, J. Biol. Chem. 286(17): 15556--15564 The NAB-Brk Signal Bifurcates at JNK to Independently Induce Apoptosis and Compensatory Proliferation. [FBrf0213516] Tripura et al., 2011, Int. J. Dev. Biol. 55(6): 583--590 Regulation and activity of JNK signaling in the wing disc peripodial membrane during adult morphogenesis in Drosophila. [FBrf0216296] Show all research papers ...