Recombinant construct P{Mae-UAS.E^S}

General Information
Symbol	P{Mae-UAS.E^S}	FlyBase ID	FBtp0001319
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	0 available
Molecular map
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Rorth, 1996)
Uses	CV term Qualifiers & info Reference control construct
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference regulatory/structural DNA (Rorth, 1996) selectable marker (Rorth, 1996) structural element (Rorth, 1996) structural element (Rorth, 1996) structural element (Rorth, 1996)
Component Alleles
Allele	gypsy\su(Hw)BR³⁷⁰
Reference(s)	(Rorth, 1996)
Molecular data	Construct: 370bp KpnI-NotI gypsy\su(Hw)BR fragment. (Rorth, 1996)
Phenotypic class
Phenotype manifest in
Other information

Allele	Scer\UAS^14x
Reference(s)	(Rorth, 1996)
Molecular data	Construct: 14 copies of Scer\GAL4 binding sites. (Rorth, 1996)
Phenotypic class
Phenotype manifest in
Other information

Allele	TrlBR^cRa
Reference(s)	(Rorth, 1996)
Molecular data	Construct: Contains two copies of a 'GAGA element' which can bind Trl. (Rorth, 1996)
Phenotypic class
Phenotype manifest in
Other information

Allele	w^+mC
Reference(s)	(Rorth, 1996)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	pP{Mae-UAS.E^S} (Rorth, 1996)
descendant(s)
Comments
	construct_comment: Restriction site useful for plasmid rescue:EcoRI.Of 102 insertions, none exhibited a mutant phenotype, implying that UAS activating elements without adjacent promoter (Hsp70) sequences fail to elicit expression from proximate genes. (Rorth, 1996)
Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	E^S (Rorth, 1996) P{Mae-UAS.E^S} (FlyBase, 1996-)
Secondary FlyBase IDs

References ( 1 )
Research paper	Rorth, 1996, Proc. Natl. Acad. Sci. U.S.A. 93(22): 12418--12422 A modular misexpression screen in Drosophila detecting tissue-specific phenotypes. [FBrf0090768]

Recombinant construct P{Mae-UAS.ES}

Recombinant construct P{Mae-UAS.E^S}