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General Information
Symbol
P{GAL4::VP16-nos.UTR}
FlyBase ID
FBtp0001325
Feature type
transgenic_transposon
Component Allele(s)
Also Known As
nos-GAL4, nanos-Gal4, Nanos-Gal4:VP16, nanos-Gal4-VP16, nos-gal4-vp16, MTD-Gal4, nosGal4, nanos-Gal4::VP16, nanos-GAL4VP16, Nos-gal4VP16
Size
Expression Data
GAL4 reporter/driver
Associated insertion(s)
Molecular map
Description and Uses
Location-dependent role
Description
CV term
Qualifiers and info
Reference
transposon
Construct components
Product class / Tool use(s)
Marker(s)
Component allele Scer\GAL4VP16.nos.UTR
Product class / Tool use(s)
Regulatory region(s)
Description
nos coding sequences within nosgnosb are replaced with sequences encoding the Tag:FLAG epitope and a GAL4::VP16 driver. The construct contains approximately 700bp of the nos promoter, the nos 5' and 3' UTRs and approximately 500bp of genomic sequences 3' of nos.
Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Features
CV term
Qualifiers and info
Reference
binary
component
chimeric
gene
molecular
tag
reporter
gene
selectable
marker
Component Alleles
Reference(s)
(Asaoka et al., 2019, Basar et al., 2019, Baskar et al., 2019, Bhandari et al., 2019, Di Cara et al., 2019, ElMaghraby et al., 2019, Ge et al., 2019, Hanyu-Nakamura et al., 2019, He et al., 2019, Hughes et al., 2019, Jagannathan et al., 2019, Kong et al., 2019, Lake et al., 2019, Li et al., 2019, Moschall et al., 2019, Nilangekar et al., 2019, Park et al., 2019, Primus et al., 2019, Ray et al., 2019, Shropshire and Bordenstein, 2019, Yu et al., 2019, Yu et al., 2019, Chen et al., 2018, Chen et al., 2018, Coll et al., 2018, DeLuca and Spradling, 2018, Flora et al., 2018, Gambetta and Furlong, 2018, Harumoto and Lemaitre, 2018, Jankovics et al., 2018, Kistler et al., 2018, Morita et al., 2018, Ng et al., 2018, Osman and Pek, 2018, Santoso et al., 2018, Shukla et al., 2018, Sugimori et al., 2018, Théron et al., 2018, Zhu et al., 2018, Akishina et al., 2017, Bence et al., 2017, Chen et al., 2017, Cheng et al., 2017, Chiang et al., 2017, Goyal et al., 2017, Ji et al., 2017, Johnson et al., 2017, Kan et al., 2017, Pae et al., 2017, Puah et al., 2017, Radford et al., 2017, Shan et al., 2017, Song et al., 2017, Yang et al., 2017, Zhang et al., 2017, Blundon et al., 2016, Börner and Becker, 2016, Börner et al., 2016, Cai et al., 2016, Catrina et al., 2016, Chaturvedi et al., 2016, Guo et al., 2016, Gyuricza et al., 2016, Hayashi et al., 2016, Huang et al., 2016, Jones et al., 2016, Kowanda et al., 2016, Ku et al., 2016, Lee et al., 2016, Liu et al., 2016, Lu et al., 2016, Navarro-Costa et al., 2016, Port and Bullock, 2016, Quijano et al., 2016, Sen et al., 2016, Stepanik et al., 2016, Tan et al., 2016, Wissel et al., 2016, Wu et al., 2016, Yadav and Sarkar, 2016, Yu et al., 2016, Zhaunova et al., 2016, Zheng et al., 2016, Ables et al., 2015, Barckmann et al., 2015, Breuer and Ohkura, 2015, Chakraborty et al., 2015, Dolezal et al., 2015, Dubruille and Loppin, 2015, Eisman et al., 2015, Ghosh and Lasko, 2015, Irizarry and Stathopoulos, 2015, Jiang et al., 2015, Johnson et al., 2015, Jones and Macdonald, 2015, Kuhn et al., 2015, Lake et al., 2015, Li et al., 2015, Lim et al., 2015, Liu and Secombe, 2015, Liu et al., 2015, Liu et al., 2015, Pek et al., 2015, Pengelly et al., 2015, Radford et al., 2015, Reubens et al., 2015, Senti et al., 2015, Sienski et al., 2015, Smendziuk et al., 2015, Winkler et al., 2015, Yang et al., 2015, Yu et al., 2015, Zhang et al., 2015, Atikukke et al., 2014, Cai et al., 2014, Collins et al., 2014, Delabaere et al., 2014, Deshpande et al., 2014, DeVorkin et al., 2014, Eun et al., 2014, Gambetta and Müller, 2014, Ghosh et al., 2014, Henstridge et al., 2014, Huang et al., 2014, Jambor et al., 2014, Komori et al., 2014, Krishnan et al., 2014, Lu et al., 2014, Ma et al., 2014, Ma et al., 2014, Matsuoka et al., 2014, Minakhina et al., 2014, Morais-de-Sá et al., 2014, Shields et al., 2014, Spracklen et al., 2014, Tripathy et al., 2014, Tseng et al., 2014, Urban et al., 2014, Wallace et al., 2014, Weber et al., 2014, Wei and Lilly, 2014, Wei et al., 2014, Xiol et al., 2014, Xu et al., 2014, Azzam and Liu, 2013, Bjorum et al., 2013, Bonner et al., 2013, Colombié et al., 2013, Dix et al., 2013, Eun et al., 2013, Hira et al., 2013, Ismat et al., 2013, Jagut et al., 2013, Johnson et al., 2013, Joly et al., 2013, Kugler et al., 2013, Leibfried et al., 2013, Le Thomas et al., 2013, Marzuk et al., 2013, Mathieu et al., 2013, Miyauchi et al., 2013, Morais-de-Sá et al., 2013, Morillo Prado et al., 2013, Quinlan, 2013, Rödel et al., 2013, Rödel et al., 2013, Su et al., 2013, Tian et al., 2013, Vilmos et al., 2013, Xin et al., 2013, Yacobi-Sharon et al., 2013, Yan and McKee, 2013, Zhao et al., 2013, Bunt et al., 2012, Chen et al., 2012, Deluca and O'Farrell, 2012, Ferguson et al., 2012, Haskel-Ittah et al., 2012, Hernandez et al., 2012, Issigonis and Matunis, 2012, Ji and Tulin, 2012, Kadir et al., 2012, Kim et al., 2012, Kitazawa et al., 2012, Mulligan et al., 2012, Nagel et al., 2012, Neumüller et al., 2012, Olivieri et al., 2012, Radford et al., 2012, Radford et al., 2012, Sanghavi et al., 2012, Sano et al., 2012, Siddall et al., 2012, Sinden et al., 2012, Stinchfield et al., 2012, Takeo et al., 2012, Tran et al., 2012, Tsigkari et al., 2012, Xia et al., 2012, Yang et al., 2012, Yuan et al., 2012, Zanet et al., 2012, Becalska et al., 2011, Casper et al., 2011, Cesario and McKim, 2011, Chang et al., 2011, Cheng et al., 2011, Dubin-Bar et al., 2011, Garcia et al., 2011, Ghorbani et al., 2011, Grillo et al., 2011, Handler et al., 2011, Harris et al., 2011, Helman et al., 2011, Jambor et al., 2011, Jayanandanan et al., 2011, Lake et al., 2011, Liu et al., 2011, Michel et al., 2011, Mukai et al., 2011, Nakayama et al., 2011, Ni et al., 2011, Okegbe and DiNardo, 2011, Pane et al., 2011, Parrott et al., 2011, Pek and Kai, 2011, Senger et al., 2011, Vazquez-Pianzola et al., 2011, Wang et al., 2011, Wong et al., 2011, Wonglapsuwan et al., 2011, Zamparini et al., 2011, Zhang et al., 2011, Zheng et al., 2011, Zhu and Bhat, 2011, Anne, 2010, Anne, 2010, Bakhrat et al., 2010, Chen et al., 2010, Cho et al., 2010, Coffee et al., 2010, Doerflinger et al., 2010, Duchi et al., 2010, Flaherty et al., 2010, Gonsalvez et al., 2010, Hudson and Cooley, 2010, Joyce and McKim, 2010, Kugler et al., 2010, Lund et al., 2010, Ma et al., 2010, Monk et al., 2010, Nezis et al., 2010, Patil and Kai, 2010, Pines et al., 2010, Renault et al., 2010, Rouget et al., 2010, Singh et al., 2010, Stein et al., 2010, Takeo et al., 2010, Titen and Golic, 2010, Xia et al., 2010, Yan et al., 2010, Anderson et al., 2009, Chen et al., 2009, Dienstbier et al., 2009, Eisman et al., 2009, Fichelson et al., 2009, Fichelson et al., 2009, Guo and Wang, 2009, Iovino et al., 2009, Kao and Megraw, 2009, Li et al., 2009, Lim et al., 2009, Moore et al., 2009, Mottier-Pavie and Megraw, 2009, Nezis et al., 2009, Rewitz et al., 2009, Rhiner et al., 2009, Rutkowski and Warren, 2009, Shen et al., 2009, Sheng et al., 2009, Vilmos et al., 2009, Yatsenko et al., 2009, Zanet et al., 2009, Boylan et al., 2008, Charron et al., 2008, Cinnamon et al., 2008, Colombié et al., 2008, Dubin-Bar et al., 2008, Gervais et al., 2008, Hanyu-Nakamura et al., 2008, Hartl et al., 2008, Hou et al., 2008, Jiang et al., 2008, Jin et al., 2008, Leatherman and DiNardo, 2008, Lee et al., 2008, Lin et al., 2008, Neumuller et al., 2008, Page et al., 2008, Ratan et al., 2008, Snee et al., 2008, Sun et al., 2008, Tan et al., 2008, Technau and Roth, 2008, Von Stetina et al., 2008, Wu et al., 2008, Xu et al., 2008, Yamada et al., 2008, Yoon et al., 2008, Baum et al., 2007, Chen et al., 2007, Clark et al., 2007, Dahlgaard et al., 2007, Grimaldi et al., 2007, Jang et al., 2007, Jeffress et al., 2007, Jung and Bonini, 2007, Kalamegham et al., 2007, Lee and Cooley, 2007, Lim and Kai, 2007, Maines et al., 2007, Mische et al., 2007, Mukai et al., 2007, Murray and Saint, 2007, Pandey et al., 2007, Pane et al., 2007, Park et al., 2007, Park et al., 2007, Peretz et al., 2007, Pesin and Orr-Weaver, 2007, Petrella et al., 2007, Rickmyre et al., 2007, Sato et al., 2007, Snee et al., 2007, Song et al., 2007, Sturgill et al., 2007, Swan and Schupbach, 2007, Tadros et al., 2007, Xiang et al., 2007, Yatsenko et al., 2007, Zhang and Megraw, 2007, Abdu et al., 2006, Bolivar et al., 2006, Bullock et al., 2006, Colombani et al., 2006, Eisman et al., 2006, Frescas et al., 2006, Gilboa and Lehmann, 2006, Gorjanacz et al., 2006, Jenny et al., 2006, Le Bras and Van Doren, 2006, Lin et al., 2006, Mazzalupo and Cooley, 2006, O'Reilly et al., 2006, Rosales-Nieves et al., 2006, Sengoku et al., 2006, Sugimura and Lilly, 2006, Takeo et al., 2006, Takeo et al., 2006, Terry et al., 2006, Vagin et al., 2006, Venkei et al., 2006, Verdier et al., 2006, Wang et al., 2006, Wehr et al., 2006, Weil et al., 2006, Zaessinger et al., 2006, Anderson et al., 2005, Cui and Hawley, 2005, Cui et al., 2005, Ganguly et al., 2005, Ghosh-Roy et al., 2005, Gordon et al., 2005, Hatfield et al., 2005, Roper et al., 2005, Semotok et al., 2005, Serbus et al., 2005, Swan et al., 2005, Van De Bor et al., 2005, Wawersik et al., 2005, Xi et al., 2005, Bartoszewski et al., 2004, Casanueva and Ferguson, 2004, Ghosh-Roy et al., 2004, Gilboa and Lehmann, 2004, Hachet and Ephrussi, 2004, Hanyu-Nakamura et al., 2004, Hsouna et al., 2004, Iida and Lilly, 2004, Jeong and Kim-Ha, 2004, Kawase et al., 2004, Nelson et al., 2004, Renault et al., 2004, Renault et al., 2004, Riechmann and Ephrussi, 2004, Schulz et al., 2004, Snapp et al., 2004, Snee and Macdonald, 2004, Suzanne, 2004, Yano et al., 2004, Ye et al., 2004, Yoshida et al., 2004, Abdu et al., 2003, Chen and McKearin, 2003, Cox and Spradling, 2003, Doerflinger et al., 2003, Filardo and Ephrussi, 2003, Findley et al., 2003, Ghiglione et al., 2003, Harris et al., 2003, Jenkins et al., 2003, Kramer and Hawley, 2003, Kramer and Hawley, 2003, Kunwar et al., 2003, Laundrie et al., 2003, Manheim and McKim, 2003, Mason et al., 2003, Peterson et al., 2003, Shivdasani and Ingham, 2003, Van Doren et al., 2003, Yamashita et al., 2003, Benton et al., 2002, Ghiglione et al., 2002, Gorjanacz et al., 2002, Hudson and Cooley, 2002, Jackson and Berg, 2002, Januschke et al., 2002, Juge et al., 2002, Kashevsky et al., 2002, Kelso et al., 2002, Mason et al., 2002, Megraw et al., 2002, Polesello et al., 2002, Riechmann et al., 2002, Schulz et al., 2002, Sinka et al., 2002, Timinszky et al., 2002, Trieselmann and Wilde, 2002, Vaccari and Ephrussi, 2002, Amikura et al., 2001, Bolivar et al., 2001, Casali and Casanova, 2001, Dorn et al., 2001, Duchek and Rorth, 2001, Duchek et al., 2001, Harris and Macdonald, 2001, Huynh et al., 2001, Kiger et al., 2001, Labourier et al., 2001, Lee et al., 2001, Schulz et al., 2001, Sonoda and Wharton, 2001, Starz-Gaiano et al., 2001, Tulina and Matunis, 2001, Andrews et al., 2000, Chen et al., 2000, Grieder et al., 2000, Mata et al., 2000, Noll et al., 2000, Ruden et al., 2000, Warren, 2000, Wasser and Chia, 2000, Rorth, 1998, Santel et al., 1998, Van Doren et al., 1998, Van Doren et al., 1998, Van Doren, 1997.3.14)
Molecular data
nos coding sequences within nosgnosb are replaced with sequences encoding the Tag:FLAG epitope and a GAL4::VP16 driver. The construct contains approximately 700bp of the nos promoter, the nos 5' and 3' UTRs and approximately 500bp of genomic sequences 3' of nos.
Expression Data
Reporter Expression
No Assay Recorded
Stage
Tissue/Position (including subcellular localization)
Reference
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Information
Statement
Reference
Drives expression in female germline stem cells at all developmental stages.
On the basis of expression of P{UAS-lacZ.B} target construct, early expression is detected in posterior somatic cells, but not in pole cells. Posterior expression exhibits a posterior-to-anterior concentration gradient. Expression of P{UAS-lacZ.B} in germ cells is not detected until stage 9.
In the early embryo, Scer\GAL4nos.UTR.T:Hsim\VP16 RNA detected in pole cells and subsequently in germ cells. However, expression ofthe GAL4::VP16-responsive target construct, P{UAS-lacZ.B}, differs. Early expression is detected in posterior somatic cells, but not in pole cells. Expression of P{UAS-lacZ.B} in germ cells is not detected until stage 9.
Expression is observed in female germline stem cells at all stages and at a high level..
 
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
Comments
construct_comment: Progenitors are those of the plasmid, pP{ GAL4::VP16-nos.UTR }.
construct_comment: Identical to P{GAL4-nos.UTR}, except GAL4::VP16 used in place of GAL4. GAL4::VP16 is a much more potent activator than GAL4 (too good for some applications).
report comment: 5.1kb insert includes the nanos promoter and 5\' and 3\' UTR\'s.
Stocks (10)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (187)
Reported As
Symbol Synonym
(GAL4::VP16-nos.UTR)
GAL4-NosVP16
Gal4-Nos:VP16
Gal4::VP16-nos
Gal4:VP16-nos.UTR
Nanos-Gal-4:VP16
Nanos-Gal4:VP16
Nanos-gal4 VP16
NanosVP16-Gal4
Nos-Gal4-VP16
Nos-Gal4::VP16nos.UTR
NosGal4:VP16
NosVp16-Gal4
P(GAL4-VP16-nos)
P(Gal4::VP16-nos.UTR)
P(w+:nanosGal4:VP-16)
P(w+:nanosGal4:VP-16)
Pw(+mC)=GAL4::VP16-nos.UTRMVD2
P[nos-Gal4-VP16]
P[nos-Gal4:VP16]
P[nos-gal4-vp16]
P[nosP-Gal4:VP16-nos3''UTR]
P[nosP-nos5'-Gal4:VP16-nos3'UTR]
P[nosVP16-GAL4]
P[w[+mC]+GAL4::VP16-nos.UTR]
Pnos-GAL4::VP16
P{GAL4::V{16-nos.UTR}
P{GAL4:VP16-nos.UTR}MVD1
P{Gal4::VP16-nos.UTR}
P{NANOS GAL4 VP-16}
P{nanosP-gal4:vp16}
P{nos-Gal4-VP16}
P{nos-Gal4::VP16}
P{nos-gal4:vp16}
P{nos-gvp}
P{nosP-Gal4:VP16}
P{nosP::gal4-vp16}
P{nosp-gal4:vp16}
P{w+ GAL4::VP16-nos.UTR}
P{w+; nos-Gal4:VP16}
P{w+mC=GAL4::VP16-nos.UTR}
VP16-nanos Gal4
VP16-nos.UTR
VP16::nos-Gal4
[nos-Gal4-VP16]
mat-Gal4
maternal GAL4
maternal Gal4
nanos (nos)-Gal4
nanos GAL4
nanos GAL4VP16
nanos Gal4
nanos-GAL4 VP16
nanos-GAL4-VP16-nanos3'UTR
nanos-GAL4-driver line 4937
nanos-GAL4-vp16
nanos-GAL4VP16
nanos-Gal4-vp16
nanos-VP-16 Gal4
nanos-VP16 GAL4
nanos-VP16-Gal4
nanos-gal4-VP16
nanos::Gal4VP16
nanos::VP16-GAL4
nanos::VP16-Gal4
nanos>Gal4VP16
nanosGAL4
nanosGal4-VP16
nanosGal4::VP16
nanosGal4VP16
nos 3'' UTR-VP16-Gal4
nos Gal4-VP16
nos Gal4VP16
nos-GAL4 VP16
nos-GAL4-vp16
nos-GAL4::VP16-nos.UTR
nos-Gal4-Vp16
nos-Gal4.VP16
nos-Gal4/VP16
nos-Gal4::vp16
nos-Gal4vp16
nos-gal4:VP-16
nos::Gal4
nos::Gal4VP16
nos::VP16-GAL4
nos>GAL4.VP16
nosGal4::VP16
p-{nos-Gal4}
pNos-GAL4-VP16
Secondary FlyBase IDs
    References (470)