A Database of Drosophila Genes & Genomes

FB2012_01, released January 20th, 2012
 

Recombinant construct P{Mae-UAS.6.11}

General Information
Symbol P{Mae-UAS.6.11} FlyBase ID FBtp0001327
Feature type transgenic_transposon
Size Expression data
Associated insertions 1378 available
Molecular map
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Description
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FB2011_10
FB2012_01
Alleles
Insertions
References
All updates Click here to see a list of all updates to this record from FB2010_08 and on.
hide Description & Uses
Species synthetic construct
Location-dependent
role
mobile activating element (UASG)
Description
CV term
Qualifiers & info
Reference
Uses
CV term
Qualifiers & info
Reference
genomic insertion screen
mobile activating element
Cloning Sites
Location
Restriction sites
Reference
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Sequence (FB)
Associated Sequence Data
DDBJ /
EMBL /
GenBank
DNA sequence
Extent
 
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Total Size
Left end
Right end
Segments
Number
Orientation
Symbol
Reference
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CV term
Qualifiers & info
Reference
mobile
activating
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Allele Scer\UAS5x.hs
Reference(s) (Merriam, 1997.5.9, Schaefer et al., 2001, Crisp and Merriam, 1997)
Molecular data
Phenotypic class
Phenotype manifest in
Other information
Allele y+t7.7
Reference(s) (Merriam, 1997.5.9, Liu and Lengyel, 2000, Crisp and Merriam, 1997, Merriam et al., 1997)
Molecular data
Construct: 8kb SalI fragment carrying y+.
Contained within 7.7kb SalI-BglII fragment that includes the entire y+ gene.
Fragment containing the y gene, including 2873bp 5' and 130bp 3' flanking sequences.
5' regulatory region deletion derivative of y, 2873 nucleotides remain upstream of the transcription start site.
Phenotypic class
Phenotype manifest in
Other information
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Reporter Expression
Additional Information
Statement
Reference
Assay mode
Marker for
Reflects expression of
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progenitor(s)
descendant(s)
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report comment: Note: y+ fragment described as 7.9kb; y sequences are 7.7kb, with an additional unrelated fragment of 170bp at the 3\' end. See note for construct P{Car20y}.
construct_comment: Reference: John Merriam, personal communication to FlyBase.Designed to be used for modular misexpression screen.GAL4-activated transcription from Hsp70 promoter is toward P5\', extending into adjacent genomic sequences.
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Bloomington
Kyoto
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Other Crossreferences
Linkouts
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Reported As
Symbol Synonym
P[y[+] UAS]
P[y+ UAS]
Secondary FlyBase IDs
hide References ( 28 )
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hide Recent research papers ( 1 )
Schneiderman et al., 2010, PLoS Genet. 6(9): e1001095
Perturbation Analysis of Heterochromatin-Mediated Gene Silencing and Somatic Inheritance. [FBrf0211786]