A Database of Drosophila Genes & Genomes

FB2008_07, released August 8, 2008
 

Recombinant construct P{Ubi-GFP.D}

General Information
Symbol P{Ubi-GFP.D} FlyBase ID FBtp0001355
Feature type transgenic_transposon Created / Updated 1997-12-29/2005-04-28
Size Expression data GFP reporter
Location-dependent role Associated insertions 7 available
Molecular map
hide Description & Uses
Species synthetic construct
Description
CV term
Qualifiers & info
Reference
transposon
P-element
(Davis et al., 1995, Lloyd et al., 2002)
Uses
CV term
Qualifiers & info
Reference
characterization
Ubi-p63E
(Davis et al., 1995)
Cloning Sites
Location
Restriction sites
Reference
hide Sequence Data
Sequence (FB)
Sequence
Associated Sequence Data
DDBJ /
EMBL /
GenBank
DNA sequence
Extent
 
hide Segments & Size
Total Size
Left end
Right end
Segments
Number
Orientation
Symbol
Reference
hide Features
CV term
Qualifiers & info
Reference
general
promoter
(Davis et al., 1995)
chimeric
gene
(Davis et al., 1995)
selectable
marker
(Davis et al., 1995)
reporter
gene
(Davis et al., 1995)
hide Component Alleles
hidew+mC
Reference(s) (Davis et al., 1995, Lloyd et al., 2002)
Molecular data
Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
 
4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site.
(Pirrotta, 1988)
Minigene of the white cDNA.
(Chung et al., 1993)
Deletion of w+mC 5' flanking sequences to position -305.
(Qian and Pirrotta, 1995)
Phenotypic class
courtship defective | male
(Lloyd et al., 2002, Hing and Carlson, 1996)
visible | dominant
(True et al., 1996)
visible | recessive
(Roseman et al., 1993)
eye color defective
(Soldatov et al., 1999, Hagstrom et al., 1996)
eye color defective, with Scer\GAL4Act5C.PI, wSym.Scer\UAS, wa.Sym.Scer\UAS
(Giordano et al., 2002)
Phenotype manifest in
pigment cell
(Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995)
pigment cell, with Scer\GAL4Act5C.PI, wSym.Scer\UAS, wa.Sym.Scer\UAS
(Giordano et al., 2002)
Other information
hideAvic\GFPUbi-p63E.PD
Reference(s) (Davis et al., 1995, Baena-Lopez et al., 2005, Baena-Lopez, 2005, Beccari et al., 2002, Janody et al., 2003, Erkner et al., 2002, Roch et al., 2002, Genova and Fehon, 2003, Suzanne et al., 2001, Okada et al., 2002, Aldridge et al., 2007, Parker et al., 2002, Lee and Adler, 2004, Schulz et al., 2002, Dominguez et al., 2004, Roch and Akam, 2000, Baeg et al., 2001, Treisman, 2001, Geng et al., 2000, Duchek and Rorth, 2001, Bilder et al., 2000, Spencer et al., 1998, Minami et al., 1999, Domingos et al., 2004, Joulia et al., 2005, Vereshchagina et al., 2004, Brand, 1999, Strutt, 2001, Roch et al., 1998, Tapon et al., 2001, Dobens et al., 2001, Hodin and Riddiford, 1998, Roper and Brown, 2004, Liu et al., 2003, Evans and Cline, 2007, Chotard et al., 2005, Bazigou et al., 2007, Lu and Bilder, 2005, Bazigoui et al., 2007)
Molecular data
Construct: 1980bp of Ubi-p63E promoter (and first four amino acids) directs the expression of full length Avic\GFP followed by Hsp27 polyadenylation sequence.
(Davis et al., 1995)
Phenotypic class
Phenotype manifest in
Other information
hide Expression Data
Reporter Expression
Stage
Tissue/Position
Reference
  larval stage | third instar
  imaginal disc
(Roch et al., 1998)
  embryonic stage
 
(Davis et al., 1995)
  embryonic stage
  ubiquitous
(Davis et al., 1995)
Additional Information
Statement
Reference
Shows bright green fluorescence in both nucleus and cytoplasm. There is a long delay between zygotic expression and appearance of fluorescence.
(Davis et al., 1995)
In third instar larva, ubiquitous expression throughout imaginal discs. Used as marker for FRT-induced mitotic clones.
(Roch et al., 1998)
Assay mode
distribution deduced from reporter
(Roch et al., 1998)
distribution deduced from reporter
(Davis et al., 1995)
distribution deduced from reporter
(Davis et al., 1995)
Marker for
Reflects expression of
hide Progenitors & Descendants
progenitor(s)
(Davis et al., 1995)
descendant(s)
hide Comments
report comment: The Ubi::GFP fusion protein is highly fluorescent when maternally supplied, but there is a long delay between zygotic expression and appearance of fluorescence.
(Davis et al., 1995)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
[w+mC=Ubi-GFP]
(Dobens et al., 2001)
nuclear ubiquitin-GFP
(Schulz et al., 2002)
P[hs-w[+]]
(Lloyd et al., 2002)
P[hs-w+]
(Lloyd et al., 2002)
P[Ubi-GFP]
(Parker et al., 2002)
P[UbiGFP]
(Tapon et al., 2001)
P[ubn-GFP]
(Spencer et al., 1998)
P{@w+mC@ @GFPUbi-p.PD@=@Ubi-GFP@}
(FlyBase, 1996-)
P{Ub::GFP w+mC}
(Evans and Cline, 2007)
P{Ubi-GFP.D}
(FlyBase, 1996-, Aldridge et al., 2007)
P{Ubi-GFP}
(FlyBase, 1996-)
P{w+mC=ubi-GFP}
(Domingos et al., 2004)
P{w+mC=Ubi-GFP}
(Genova and Fehon, 2003)
PUbGFP
(Davis et al., 1995, Brand, 1999)
Ub-GFP
(Liu et al., 2003, Minami et al., 1999, Chotard et al., 2005)
UBGFP
(Suzanne et al., 2001)
Ubi::GFP
(Roper and Brown, 2004)
Ubi-GFP
(Lee and Adler, 2004, Janody et al., 2003, Okada et al., 2002, Strutt, 2001, Duchek and Rorth, 2001, Geng et al., 2000, Bazigou et al., 2007, Bazigoui et al., 2007)
ubi-GFP
(Dominguez et al., 2004)
ubiGFP
(Erkner et al., 2002)
ubi-GFP{w+mC}
(Dominguez et al., 2004)
ubiquitinGFP
(Beccari et al., 2002)
ubiquitin-GFP
(Baeg et al., 2001, Bilder et al., 2000)
ubiquitous nuclear GFP
(Roch et al., 2002)
UbqnGFP
(Roch and Akam, 2000)
unnamed
(Lu and Bilder, 2005)
Secondary FlyBase IDs
    hide References ( 51 )
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    hide Recent research papers ( 3 )
    Aldridge et al., 2007, Fly 1(1): 38--46
    Roles for Drp1, a dynamin-related protein, and milton, a kinesin-associated protein, in mitochondrial segregation, unfurling, and elongation during Drosophila spermatogenesis. [FBrf0200064]
    Bazigou et al., 2007, Cell 128(5): 961--975
    Anterograde jelly belly and Alk receptor tyrosine kinase signaling mediates retinal axon targeting in Drosophila. [FBrf0192674]
    Evans and Cline, 2007, Genetics 175(2): 631--642
    Drosophila melanogaster male somatic cells feminized solely by TraF can collaborate with female germ cells to make functional eggs. [FBrf0194910]
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