Recombinant construct P{Ubi-GFP.nls}

General Information
Symbol	P{Ubi-GFP.nls}	FlyBase ID	FBtp0001356
Feature type	transgenic_transposon	Created / Updated	1997-12-29/1997-12-30

Size		Expression data	lacZ reporter,GFP reporter
Location-dependent role		Associated insertions	7 available
Molecular map
Description & Uses
Species	synthetic construct
Description	CV term Qualifiers & info Reference transposon P-element (Davis et al., 1995)
Uses	CV term Qualifiers & info Reference characterization Ubi-p63E (Davis et al., 1995)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Sequence
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference general promoter (Davis et al., 1995) chimeric gene (Davis et al., 1995) selectable marker (Davis et al., 1995) molecular tag (Davis et al., 1995) reporter gene (Davis et al., 1995)
Component Alleles
w^+mC
Reference(s)	(Davis et al., 1995)
Molecular data	Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors. 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Minigene of the white cDNA. (Chung et al., 1993) Deletion of w^+mC 5' flanking sequences to position -305. (Qian and Pirrotta, 1995)
Phenotypic class	courtship defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^Sym.Scer\UAS, w^{a.Sym.Scer\UAS} (Giordano et al., 2002)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^Sym.Scer\UAS, w^{a.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Avic\GFP^{Ubi-p63E.T:Ecol\lacZ,T:Zzzz\nls1}
Reference(s)	(Davis et al., 1995, Peri et al., 2002, Kumar et al., 2001, Fujise et al., 2003, Caceres and Nilson, 2005, Doerflinger et al., 2003, Lin et al., 2003, Guichet et al., 2001, Culi and Mann, 2003, Beall et al., 2002, Geisbrecht and Montell, 2004, Luschnig et al., 2004, Fichelson and Gho, 2003, Bai and Montell, 2002, Lin et al., 2004, Sato and Kornberg, 2002, Gorjanacz et al., 2002, Huynh et al., 2004, MacDougall et al., 2001, Methot and Basler, 2001, Huynh et al., 2001, Yannoni and White, 1999, Luschnig et al., 2000, Namba and Minden, 1999, Brand, 1999, Lopez-Schier and St. Johnston, 2001, Wilkie and Davis, 2001, Torres et al., 2003, Jones et al., 2006, Delanoue and Davis, 2005)
Molecular data	Construct: 1980bp of Ubi-p63E promoter (and first four amino acids) directs the expression of a 9 amino acid nuclear localization sequence fused to 40 amino acids of Ecol\lacZ (from its N-terminus) fused to full length Avic\GFP followed by Hsp27 polyadenylation sequence. (Davis et al., 1995)
Phenotypic class
Phenotype manifest in
Other information
Expression Data
Reporter Expression
Stage Tissue/Position Reference embryonic stage ubiquitous (Davis et al., 1995) embryonic stage (Davis et al., 1995)
Additional Information
Statement Reference Shows highly fluorescent green nuclei and less fluorescent cytoplasm. (Fusion protein contains nuclear localization signal.) There is a long delay between zygotic expression and appearance of fluorescence. (Davis et al., 1995) In early embryo, used as nuclear marker for identification of cells within mitotic domains. (Namba and Minden, 1999)

Assay mode	distribution deduced from reporter (Davis et al., 1995) distribution deduced from reporter (Davis et al., 1995)
Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	pP{Ubi-GFP.nls} (Davis et al., 1995)
descendant(s)
Comments
	report comment: The Ubi::lacZ::GFP fusion protein is highly fluorescent when maternally supplied, but there is a long delay between zygotic expression and appearance of fluorescence. (Davis et al., 1995)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	GFPnls (Huynh et al., 2001) nlsGFP (Torres et al., 2003, Doerflinger et al., 2003, Wilkie and Davis, 2001, MacDougall et al., 2001) NLS-GFP (Gorjanacz et al., 2002) nls-GFP (Delanoue and Davis, 2005) nlsGFPM (Kumar et al., 2001) P(Ubi:GFP.nls) (Jones et al., 2006) P[Ubi-GFP^nls] (Lin et al., 2003) P[ubi-nlsGFP] (Methot and Basler, 2001) P[w+; ubi:GFPnls] (Huynh et al., 2004) P{@w^+mC@ @GFP^{Ubi-p.T:lacZ.T:nls1}@=@Ubi-GFP.nls@} (FlyBase, 1996-) P{Ubi-GFP.nls} (FlyBase, 1996-) P{w^+mC=UbiGFP.nls} (Yannoni and White, 1999) PUbnlsGFP (Brand, 1999) Ubi-GFP.nls (Culi and Mann, 2003) Ubi-GFPnls (Caceres and Nilson, 2005, Lin et al., 2004, Namba and Minden, 1999) ubi-nls-GFP (Geisbrecht and Montell, 2004) ubi-nlsGFP (Luschnig et al., 2004, Bai and Montell, 2002, Sato and Kornberg, 2002, Lopez-Schier and St. Johnston, 2001, Luschnig et al., 2000) Ubi-nlsGFP (Fujise et al., 2003) ubi-nls GFP (Peri et al., 2002, Guichet et al., 2001) ubq-nls::GFP (Fichelson and Gho, 2003)
Secondary FlyBase IDs

References ( 42 )

Generate a list of	All references relating to this transgenic transposon ( 42 )

List References by type	Research paper ( 40 ) Review ( 1 ) FlyBase analysis ( 1 )
Recent research papers ( 1 )
	Jones et al., 2006, Genetics 173(2): 793--808 Characterization of Drosophila mini-me, a gene required for cell proliferation and survival. [FBrf0193720] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2006 Show reviews ...