Recombinant construct P{CXY21.29+4a}

General Information
Symbol	P{CXY21.29+4a}	FlyBase ID	FBtp0001382
Feature type	transgenic_transposon

Size		Expression data	lacZ reporter
Associated insertions	1 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role	carries FRT site(s) (Harrison and Perrimon, 1993)
Description	CV term Qualifiers & info Reference transposon P-element (Harrison and Perrimon, 1993)
Uses	CV term Qualifiers & info Reference analytical tool mosaic analysis (Harrison and Perrimon, 1993)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference chimeric gene (Harrison and Perrimon, 1993) reporter gene (Harrison and Perrimon, 1993) selectable marker (Harrison and Perrimon, 1993) selectable marker (Harrison and Perrimon, 1993)
Component Alleles
Allele	Ecol\lacZ^αTub84B.PHb
Reference(s)	(Harrison and Perrimon, 1993)
Molecular data	αTub84B promoter sequences drive Ecol\lacZ expression. (Harrison and Perrimon, 1993)
Phenotypic class
Phenotype manifest in
Other information

Allele	w^+mC
Reference(s)	(Harrison and Perrimon, 1993)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information

Allele	y^+mDint2
Reference(s)	(Harrison and Perrimon, 1993)
Molecular data	Construct: 5.2kb y⁺ gene including 2.8kb of 5' flanking sequences and 0.13kb of 3' sequences and lacks the tissue-specific bristle and tarsal claw enhancers. (Roseman et al., 1995) 5.2kb y gene fragment containing 2.8kb of 5' flanking and 0.13kb of 3' flanking DNA and lacking the intron that is the location of the tissue-specific bristle and tarsal claw enhancers. (Roseman et al., 1995) 5kb intronless SalI fragment containing the y gene. (Harrison and Perrimon, 1993) 5.2kb SalI y gene intronless fragment containing 2.9kb of 5' flanking and 0.3kb of 3' flanking DNA. (Patton et al., 1992)
Phenotypic class	body color defective (Ring and Garza, 2003.6.10, Roseman et al., 2001) visible (Roseman et al., 2001)
Phenotype manifest in	adult cuticle (Roseman et al., 2001) arista (Ring and Garza, 2003.6.10) macrochaeta (Ring and Garza, 2003.6.10) unguis (Ring and Garza, 2003.6.10)
Other information
Expression Data
Reporter Expression
distribution deduced from reporter Stage Tissue/Position (including subcellular localization) Reference organism \| ubiquitous (Zhang and Kalderon, 2000)
Additional Information
Statement Reference Used as marker for mosaic analysis in embryos. (Zhang and Kalderon, 2000)

Marker for
Reflects expression of	αTub84B (Zhang and Kalderon, 2000)
Progenitors & Descendants
progenitor(s)	P{3'CXY21.4a} (Harrison and Perrimon, 1993) P{5'CXY21.29} (Harrison and Perrimon, 1993)
descendant(s)
Comments
	construct_comment: Equivalent to regenerated P{CXY21} element, with regenerated lacZ marker. (Harrison and Perrimon, 1993)
Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{CXY21.29+4a} (FlyBase, 1996-)
Secondary FlyBase IDs

References ( 1 )
Research paper	Harrison and Perrimon, 1993, Curr. Biol. 3(7): 424--433 Simple and efficient generation of marked clones in Drosophila. [FBrf0057940]