Recombinant construct P{Act5C-GAL4}
| General Information | |||
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| Symbol | P{Act5C-GAL4} | FlyBase ID | FBtp0001406 |
| Feature type | transgenic_transposon | ||
| Size | 12.481 | Expression data | GAL4 reporter/driver |
| Associated insertions | 3 available | ||
| Molecular map | |||
Recent Updates
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| Description |
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This section contains items that were added to this record for each release.
It currently only tracks new links between this FlyBase report and other
FlyBase data classes (e.g. genes, references, stocks) or controlled
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| FB2013_03 | |||
| FB2013_02 |
Controlled Vocabulary Terms
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| All updates | Click here to see a list of all updates to this record from FB2010_08 and on. | ||
Description & Uses
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| Species | synthetic construct | ||
| Location-dependent
role |
carries FRT site(s)
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| Description |
CV term
Qualifiers & info
Reference
transposon
P-element
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| Uses |
CV term
Qualifiers & info
Reference
analytical tool
mosaic analysis
binary system (regulatory)
Act5C
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| Cloning Sites |
Location
Restriction sites
Reference
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Sequence Data
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| Sequence (FB) | FlyBase-compiled | ||
| Associated Sequence Data | |||
| DDBJ
/
EMBL / GenBank |
DNA sequence
Extent
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Segments & Size
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| Total Size |
12.481
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| Left end |
P5\'
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| Right end |
P3\'
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| Segments |
Number
Orientation
Symbol
2
+
3
4
+
5
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| Reference | (Ito et al., 1997) | ||
Features
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CV term
Qualifiers & info
Reference
binary
component
chimeric
gene
general
promoter
partial
rescue
reporter
gene
selectable
marker
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Component Alleles
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| Allele | Scer\GAL4Act5C.PI | ||
| Reference(s) | (Ito et al., 1997, Tue et al., 2011, Zhong et al., 2011, Baumann et al., 2010, Harterink et al., 2011, Stavropoulos and Young, 2011, Shah et al., 2012, Mitonaka et al., 2007, Port et al., 2011, Inada et al., 2011, Xu et al., 2012, Birner-Gruenberger et al., 2012, Verma et al., 2012, Fernandez-Ayala et al., 2009, Wen et al., 2012, McKinley et al., 2012, Terhzaz et al., 2012, Keegan et al., 2011, Lang et al., 2012, Shao et al., 2011, Reddy and Irvine, 2011, Zhang et al., 2011, Benna et al., 2010, Baonza and Freeman, 2005, Zielke et al., 2006, Nieratschker et al., 2009, Luders et al., 2003, Greeve et al., 2004, Martin-Lanneree et al., 2006, Yoshida et al., 2004, Baena-Lopez et al., 2005, Hayashi and Carthew, 2004, Brumby et al., 2002, del Alamo Rodriguez et al., 2002, Giordano et al., 2002, Baena-Lopez et al., 2005, Major and Irvine, 2005, Baonza and Freeman, 2001, Uhlirova et al., 2005, Palmer et al., 1999, Bessa and Casares, 2005, Drapeau et al., 2003, Fujise et al., 2003, Kolzer et al., 2003, Buckles et al., 2001, Schaefer et al., 2001, Takeo et al., 2005, Bastock et al., 2003, Kramer et al., 2003, Adachi-Yamada et al., 1999, Silver et al., 2005, Pitman et al., 2002, Cohen et al., 2002, Rauskolb, 2001, Subramanian et al., 2003, Keegan et al., 2005, Zordan et al., 2006, Dai et al., 2003, MacKay et al., 2003, Goto et al., 2001, Selva et al., 2001, Missirlis et al., 2001, van de Bor et al., 2002, Mongelard et al., 2002, Lee and Adler, 2002, Duchek et al., 2001, Missirlis et al., 2002, Tschaepe et al., 2002, Walker et al., 2006, Magie et al., 2002, Cavodeassi et al., 2002, Eun et al., 2007, Furuhashi et al., 2006, Mohan et al., 2007, Niki et al., 2006, Niki, 2006, Anderson et al., 2006, Stabell et al., 2006, Cherbas et al., 2003, Green et al., 2003, Nakagoshi et al., 2002, Glise et al., 2002, Zraly et al., 2003, Chou and Chien, 2002, Okajima and Irvine, 2002, Lengyel and Merriam, 2001, Evans et al., 2002, Akieda and Merriam, 2001, Bardot et al., 2002, Belote and Fortier, 2002, Enerly et al., 2002, Reichhart et al., 2002, Hennig and Neufeld, 2002, Kashevsky et al., 2002, Potter et al., 2002, Callus and Mathey-Prevot, 2002, Matakatsu and Blair, 2004, Chang et al., 2002, Chao et al., 2004, Alvarado et al., 2004, Fujii and Amrein, 2002, Niwa et al., 2004, Griffitts et al., 2003, Singh et al., 2004, Bai and Montell, 2002, Baonza and Freeman, 2002, Fitzpatrick et al., 2002, Araki et al., 2003, Wang et al., 2009, Zhu et al., 2007, Singh et al., 2002, Frankfort and Mardon, 2004, Enerly et al., 2003, Adachi-Yamada and O'Connor, 2002, Xu et al., 2002, del Alamo Rodriguez et al., 2004, Botella et al., 2004, Yan et al., 2004, Warner and Longmore, 2009, Elalayli et al., 2008, Roch and Akam, 2000, Dolezal et al., 2003, Kandachar et al., 2008, Mason et al., 2003, Alcedo et al., 2000, Chang et al., 2003, Pagliarini and Xu, 2003, Huang and Rubin, 2000, Kramerova et al., 2000, Cavodeassi et al., 2000, Bao and Cagan, 2005, Kumar and Moses, 2001, Geng et al., 2000, Hirosawa-Takamori et al., 2000, Flores et al., 2000, Liu and Lengyel, 2000, Chen et al., 2000, Tanimoto et al., 2000, Liu et al., 2000, Moon et al., 2005, Baonza et al., 2000, Baonza et al., 2000, Rauskolb et al., 1999, Baonza and Garcia-Bellido, 2000, Cavodeassi et al., 1999, Sawamoto et al., 1999, Goto and Hayashi, 1999, Rauskolb and Irvine, 1999, Adachi-Yamada et al., 2005, Adachi-Yamada et al., 1999, Aldaz et al., 2005, Han et al., 2005, von Stein et al., 2005, Okumura et al., 2005, Tanentzapf et al., 2000, Niwa et al., 2004, Somogyi and Rorth, 2004, Nakano et al., 2004, Reiling and Hafen, 2004, Adam and Montell, 2004, Perez-Garijo et al., 2004, Pinheiro and Montell, 2004, Hori et al., 2004, Piccin et al., 2001, Yang and Sehgal, 2001, Keisman and Baker, 2001, Ekengren et al., 2001, Sonoda and Wharton, 2001, Tomoyasu et al., 1998, Strutt, 2001, Potter et al., 2001, Papayannopoulos et al., 1998, Loren et al., 2001, Dominguez et al., 1998, Bloomington Drosophila Stock Center, 1998.9.14, Behr et al., 2003, Galloni, 2003, Kango-Singh et al., 2003, Shivdasani and Ingham, 2003, Jin et al., 2003, Patel et al., 2003, Brennecke et al., 2003, Sabatier et al., 2003, Pastor-Pareja et al., 2004, Ji and Clark, 2006, Hickey et al., 2006, Atkey et al., 2006, Hofmeyer, 2006, Matakatsu and Blair, 2006, Gibson et al., 2006, McEwen and Peifer, 2005, Mosimann et al., 2006, Rives et al., 2006, Wells et al., 2006, Kent et al., 2006, Bazigou et al., 2007, Bartscherer et al., 2006, Oshima, 2006, Celotto et al., 2006, Abdu et al., 2006, Lyalin et al., 2006, Gronke et al., 2005, Mulinari et al., 2006, Jacobsen et al., 2006, Bazigou et al., 2007, Kuranaga et al., 2006, Stergiopoulos et al., 2009, Voigt et al., 2002, Takeuchi et al., 2009, Vidal et al., 2006, Igaki et al., 2006, Niwa et al., 2008, Keyser et al., 2007, Oh and Irvine, 2009, Walters et al., 2006, Brown et al., 2006, Zhai et al., 2006, Sage et al., 2008, Yang et al., 2005, Yasunaga et al., 2006, Glittenberg et al., 2006, Ninov et al., 2007, Song et al., 2007, Hagedorn et al., 2006, He et al., 2005, Polaski et al., 2006, Mao et al., 2006, Jafar-Nejad, 2005, Seto and Bellen, 2006, Muhlig-Versen et al., 2005, Ahmad and Henikoff, 2001, Chotard et al., 2005, Kamimura et al., 2006, Chung et al., 2007, Ishikawa et al., 2005, Park et al., 2006, Strutt et al., 2004, Araki et al., 2005, Stabell et al., 2007, Kirilly et al., 2005, Lee and Cooley, 2007, Wang et al., 2007, Martin et al., 2005, Félix et al., 2007, Taylor et al., 2004, Maxwell et al., 2008, Takahashi et al., 2007, Rajendran et al., 2008, Ratan et al., 2008, Eun et al., 2008, Markstein et al., 2008, Draper et al., 2007, Hallson et al., 2008, Rollins et al., 2004, Kirilly et al., 2005, Norgate et al., 2006, Thompson and Travers, 2008, Cliffe et al., 2009, Yoshioka et al., 2008, Venkatachalam et al., 2008, Steiger et al., 2008, Mandalaywala et al., 2008, Lee et al., 2009, Yan et al., 2009, Ford et al., 2007, Lin et al., 2009, Seigle et al., 2008, Liu et al., 2009, Arya and Lakhotia, 2008, Eivers et al., 2009, Bretman et al., 2010, Wang and Kalderon, 2009, Svendsen et al., 2009, Grönke et al., 2009, Morris et al., 2006, Le Mée et al., 2008, Mallik and Lakhotia, 2009, Shahidullah et al., 2009, Das Thakur et al., 2010, Ogasawara et al., 2007, Wagner et al., 2010, Rousset et al., 2010, Ballard et al., 2010, Villányi et al., 2008, Hua et al., 2010, Gluderer et al., 2010, Alfonso-Parra and Maggert, 2010, El Chamy et al., 2008, Sekyrova et al., 2010, Matusek et al., 2008, Gou et al., 2010, Fraichard et al., 2010, Nakamura et al., 2010, Kim et al., 2010, Tsai and Sun, 2004, Igaki et al., 2009, Agrawal et al., 2009, Ni et al., 2009, Peralta et al., 2009, Loveall and Deitcher, 2010, Bodai et al., 2007, Mallik and Lakhotia, 2010, Takahashi et al., 2010, Puseenam et al., 2009, Sharma et al., 2008, Tang et al., 2010, Sanders et al., 2010, Rhiner et al., 2010, Spain et al., 2010, Hall et al., 2010, Carra et al., 2010, Kim et al., 2010, Tsuda et al., 2010, Beinert et al., 2004, Ferretti et al., 2010, Dubin-Bar et al., 2008, Friggi-Grelin et al., 2003, Saja et al., 2010, Honegger et al., 2008, Marinho et al., 2011, Wang et al., 2008, Klusza and Deng, 2010, Köhler et al., 2009, Cho and Fischer, 2011, Yu et al., 2011, Wang et al., 2010, Onorati et al., 2011, Hector et al., 2009, Brideau and Barbash, 2011, Tan et al., 2011, Kawamori and Yamaguchi, 2011, Nishimura et al., 2008, Montagne et al., 2010, Rauskolb et al., 2011, Schwedes et al., 2011, Renfranz et al., 2010, Meyer et al., 2011, Colinet et al., 2010, Colinet et al., 2010, Cui et al., 2008, Du et al., 2011, Patalano et al., 2009, Benna et al., 2009, Terhzaz et al., 2010, Johnson et al., 2010, Xie et al., 2010, Bosveld et al., 2012, Tsigkari et al., 2012, Isaji et al., 2011, Luo et al., 2012, Ji and Tulin, 2012, Daenzer et al., 2012, Saleem et al., 2012, Lawlor et al., 2012, Gault et al., 2012, Herz et al., 2012, Dahal et al., 2012, Panz et al., 2012, Li, 2010, Leitao-Goncalves et al., 2012, Klapholz et al., 2009, Schotman et al., 2008, Charron et al., 2008, Zheng et al., 2010, Nam et al., 2012, Chen et al., 2011, Harder et al., 2008, Degennaro et al., 2011, Tsuda et al., 2010, Lorbeck et al., 2011, Saini et al., 2011, Sansone and Blumenthal, 2012, Hyun and Hashimoto, 2011, Thackray et al., 2012, Galletti et al., 2009, Muñiz Ortiz et al., 2011, Humphrey et al., 2009, Lee and Fischer, 2012, Micale et al., 2009) | ||
| Molecular data |
A functional Scer\GAL4[Act5C.PI] (P{Act5C-GAL4}) is reconstituted by FRT-mediated recombination between P{y.FRT.GAL4}52B and P{Act5C.FRT}52B at identical chromosomal locations in trans to each other.
Scer\GAL4[Act5C.PI] (P{Act5C-GAL4}) can be reconstituted by FRT-mediated recombination between P{y.FRT.GAL4}52B and P{Act5C.FRT}52B at identical chromosomal locations in trans to each other.
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| Phenotypic class |
lethal, with baiKK108684
lethal, with Caf1GD11258
lethal, with CG9650CTG-240.4
lethal, with CHOp24GD3225
lethal, with CHOp24KK107384
lethal, with D12.AD12.A
lethal, with E2fScer\UAS.cNa
lethal, with ecaKK109376
lethal, with EcRA.Scer\UAS
lethal, with EcRB1.Scer\UAS
lethal, with EcRB2.Scer\UAS
lethal, with GaleNIG.12030R
lethal, with Hr96Scer\UAS.cSa
lethal, with Hrb98DEGD14939
lethal, with opmKK107517
lethal, with p24-1GD3321
lethal, with p24-1KK108359
lethal, with rprScer\UAS.cZa
lethal, with tncEY16369
long lived, with Hsp22EP3247
small body, with Fer1HCHDN.Scer\UAS
(Li, 2010)
viable, with CG31787GD1759
viable, with svpScer\UAS.cKa
viable, with Tip60s-s.Scer\UAS
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| Phenotype manifest in |
actin filament & border follicle cell | somatic clone, with Rac1V12.Scer\UAS
adult cuticle & adult abdomen, with Nop60BdsRNA.Sym.Scer\UAS
axon & photoreceptor cell R8 | somatic clone, with AlkEC.Scer\UAS.T:Hsap\MYC
axon & photoreceptor cell R8 | somatic clone, with standsRNA.extra.Scer\UAS, standsRNA.intra.Scer\UAS
cell-cell adherens junction & dorsal mesothoracic disc, with DlScer\UAS.cDa
dorsal mesothoracic disc & filamentous actin | somatic clone, with Nint.SH.Scer\UAS
dorsal mesothoracic disc & peripodial epithelium & prepupa, with pucE69, pucScer\UAS.cMa
dorsal mesothoracic disc & peripodial epithelium & prepupa, with pucScer\UAS.cMa
dorsal mesothoracic disc & peripodial stalk & prepupa, with pucE69, pucScer\UAS.cMa
dorsal mesothoracic disc & peripodial stalk & prepupa, with pucScer\UAS.cMa
eye, with EgfrDN.Scer\UAS
filamentous actin & dorsal mesothoracic disc, with DlScer\UAS.cDa
filamentous actin & embryonic/first instar larval cuticle | ectopic | somatic clone, with ovoScer\UAS.cPa
follicle cell & actin filament | somatic clone, with Pvrλ.Scer\UAS.T:Hsap\MYC
leg, with hyxEY06898
leg & adult cuticle | somatic clone, with drmScer\UAS.cGa
leg & adult cuticle | somatic clone, with oddScer\UAS.cHa
leg & trichome | somatic clone, with esgScer\UAS.cFa
leg & trichome | somatic clone, with hthScer\UAS.cPa
medulla cortex & glial cell | somatic clone, with gcm2Scer\UAS.cCa
medulla cortex & glial cell | somatic clone, with gcmScer\UAS.cJa
mesothoracic tergum & sensory organ cell | ectopic, with DlScer\UAS.cJa
microchaeta & mesothoracic tergum | somatic clone, with AmunScer\UAS.T:Disc\RFP-mRFP
neuroblast | supernumerary & larval brain | somatic clone, with NICN.Scer\UAS
ommatidium, with ayUAS
ommatidium, with RhebAV4
S2 cell-line & filamentous actin, with ik2DN.Scer\UAS
scutum & macrochaeta, with tkvQ199D.Scer\UAS.cTa
tibia, with eyScer\UAS.cHa
wing, with eyScer\UAS.cHa
wing, with tncEY16369
wing, with ydsRNA.Scer\UAS
wing & sensillum campaniformium | supernumerary | somatic clone, with gcmScer\UAS.cvdBa
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| Other information | |||
| Allele | w+mC | ||
| Reference(s) | (Pirrotta, 1988, Ito et al., 1997, Gronke et al., 2005) | ||
| Molecular data |
Minigene of the white cDNA.
4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding
the transcription start site.
Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the
Casper series of vectors.
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| Phenotypic class | |||
| Phenotype manifest in |
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| Other information | |||
Expression Data
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| Reporter Expression | |||
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distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
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| Additional Information | |||
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Statement
Reference
Designed for use in mosaic analyses; used to drive gene of interest in Scer\FLP1-generated clones.
Transiently generated during mosaic analyses; ubiquitous expression throughout region of the generated clone, but not in rest
of body (cell autonomous).
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| Marker for | |||
| Reflects expression of | |||
Progenitors & Descendants
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| progenitor(s) | |||
| descendant(s) | |||
Comments
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construct_comment: Contains a single FRT site.
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Stocks Listed in FlyBase
( 7 )
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| Bloomington | |||
| Kyoto | |||
External Crossreferences & Linkouts
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| Other Crossreferences | |||
| Linkouts | |||
Synonyms & Secondary IDs
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| Reported As | |||
| Symbol Synonym |
(act)-gal4
Ac5C>y+>Gal4
Ac>>GAL4
Act
Act-5c
ACT5C
Act5c
Act5C::Gal4
act5C>GAL4
Act5C>y+>gal4
Act5Cflip-outGal4
Act5CGal4
Act5C-Gal4
(Pinheiro and Montell, 2004, Giordano et al., 2002, Yang and Sehgal, 2001, Chen et al., 2000, Walker et al., 2006, Thompson and Travers, 2008, Stabell et al., 2007, Elalayli et al., 2008, Arya and Lakhotia, 2008, Gluderer et al., 2010, Saja et al., 2010, Honegger et al., 2008, Yu et al., 2011, Meyer et al., 2011, Patalano et al., 2009, Benna et al., 2009, Birner-Gruenberger et al., 2012, Charron et al., 2008, Luo et al., 2012)
Act5C-GAL4
(Chang et al., 2003, Jin et al., 2003, Zraly et al., 2003, Hennig and Neufeld, 2002, Chang et al., 2002, Huang and Rubin, 2000, Alcedo et al., 2000, Fernandez-Ayala et al., 2009, Abdu et al., 2006, Jacobsen et al., 2006, Furuhashi et al., 2006, Stabell et al., 2006, Yoshida et al., 2004, Onorati et al., 2011, Hagedorn et al., 2006, Polaski et al., 2006, Ishikawa et al., 2005, Martin et al., 2005, Taylor et al., 2004, Beinert et al., 2004, Stergiopoulos et al., 2009, Kandachar et al., 2008, Mallik and Lakhotia, 2009, Nakamura et al., 2010, Peralta et al., 2009, Loveall and Deitcher, 2010, Tang et al., 2010, Sanders et al., 2010, Carra et al., 2010, Dubin-Bar et al., 2008, Renfranz et al., 2010, Johnson et al., 2010, Xie et al., 2010, Verma et al., 2012, Shah et al., 2012, Nam et al., 2012, Tsuda et al., 2010, Muñiz Ortiz et al., 2011, Ji and Tulin, 2012)
Act5C-gal4
act5C-Gal4
Act5c-GAL4
Act5C Gal4
act5c-GAL4
act5C GAL4
Act5CGAL4
Act5cGal4
act5C-gal4
Act5c-Gal4
Act5C-GAL4(II)
act::Gal4
Act:GAL4
act:GAL4
Act5C>GAL4
Act>>GAL4
act>>GAL4
act>Gal4
act > Gal4
act>y+>gal-4
ActG4
act-GAL4
actGAL4
ActGal4
act-Gal4
Act-Gal4
Act-GAL4
ActGAL4
actin5CGal4
actin5C>
Actin5c >> Gal4
actin-5C-GAL4
Actin5c-GAL4
actin5C-Gal4
Actin5c-Gal4
actin 5C-GAL4
Actin5C-GAL4
Actin 5C-GAL4
actin 5C-Gal4
Actin5C-Gal4
Actin-5C-GAL4
actin5C-GAL4
actin5CGAL4
actin5C-gal4
Actin5cGAL4
Actin5C-gal4
Actin5-Gal4
actin::GAL4
actin>>Gal4
actin-GAL4
(Takeo et al., 2005, Botella et al., 2004, Griffitts et al., 2003, Xu et al., 2002, Geng et al., 2000, Adachi-Yamada et al., 1999, Sawamoto et al., 1999, Zhai et al., 2006, Greeve et al., 2004, Rajendran et al., 2008, Baumann et al., 2010, Steiger et al., 2008, Xu et al., 2012, Das Thakur et al., 2010, Hua et al., 2010, Wang et al., 2008, Nishimura et al., 2008, Terhzaz et al., 2012, Zhang et al., 2011, Dahal et al., 2012, Humphrey et al., 2009)
actin-Gal4
(Dolezal et al., 2003, Brennecke et al., 2003, Bardot et al., 2002, Magie et al., 2002, Akieda and Merriam, 2001, Lengyel and Merriam, 2001, Hirosawa-Takamori et al., 2000, Bazigou et al., 2007, Bartscherer et al., 2006, Abdu et al., 2006, Panz et al., 2012, Kamimura et al., 2006, Lee and Cooley, 2007, Inada et al., 2011, Oh and Irvine, 2009, Mohan et al., 2007, Wen et al., 2012, Nieratschker et al., 2009, Saleem et al., 2012, Bodai et al., 2007, Köhler et al., 2009, Meyer et al., 2011, Montagne et al., 2010, Schwedes et al., 2011, Stavropoulos and Young, 2011, Reddy and Irvine, 2011, Degennaro et al., 2011, Gault et al., 2012, Dahal et al., 2012)
actin-gal4
actin-Gal-4
Actin-Gal4
Actin-GAL4
actinGal4
Actin Gal4
Actin-GAL4}
Ay-Gal4(25)
flpout-GAL4
Gal4-Act5c
GAL4-Act5C
Gal4-Act5C
P[w+,Act5C-GAL4]
P{Act5C-GAL4}
(Ekengren et al., 2001, FlyBase, 1996-, Yang et al., 2005, Celotto et al., 2006, Jacobsen et al., 2006, Zhu et al., 2007, Martin-Lanneree et al., 2006, Wang et al., 2007, Takahashi et al., 2007, Hallson et al., 2008, Cliffe et al., 2009, Ford et al., 2007, Keyser et al., 2007, Villányi et al., 2008, Fraichard et al., 2010, Mallik and Lakhotia, 2010, Takahashi et al., 2010, Sanders et al., 2010, Sansone and Blumenthal, 2012, Daenzer et al., 2012)
P{Act5C-Gal4}
P{Act5c-Gal4}
P{Act5CGAL4}
P{Act5C - GAL4}
P{Act5C-GAL4}25F01
P{Act5C-GAL4}25FO1
P{Act5CGAL, w+}
P{GAL4.Act5C.I}
P{GAL4-Act5C(-FRT)}
P{w+mC = Act5C-GAL4}
P{w+mC=Act5C-GAL4}
Pactin-Gal4
P actin-Gal4
Pactin-GAL4
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| Secondary FlyBase IDs | |||
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References
( 379 )
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Recent research papers ( 51 )
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Recent Updates
External Crossreferences & Linkouts