Recombinant construct P{GAL4-Kr.C}

General Information
Symbol	P{GAL4-Kr.C}	FlyBase ID	FBtp0001434
Feature type	transgenic_transposon

Size		Expression data	GAL4 reporter/driver
Associated insertions	6 available
Molecular map
Recent Updates
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FB2013_03
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Castelli-Gair et al., 1994)
Uses	CV term Qualifiers & info Reference binary system (regulatory) Kr (Castelli-Gair et al., 1994) characterization Kr \| regulatory (Castelli-Gair et al., 1994)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference binary component (Castelli-Gair et al., 1994) chimeric gene (Castelli-Gair et al., 1994) reporter gene (Castelli-Gair et al., 1994) selectable marker (Castelli-Gair et al., 1994)
Component Alleles
Allele	Scer\GAL4^Kr.PM
Reference(s)	(Murthy et al., 2003, Hooper, 2003, Badenhorst, 2001, Liu et al., 2002, Wessells et al., 1999, Inbal et al., 2001, Shandala et al., 2003, Mansfield et al., 2002, Kiger et al., 2001, Schock and Perrimon, 2003, Sharrow and Tiemeyer, 2001, Colas et al., 1999, Fung et al., 2003, Rusten et al., 2002, Schock and Perrimon, 2002, Donaldson et al., 2004, Narasimha and Brown, 2004, Overton et al., 2002, Lo et al., 2002, Castelli-Gair et al., 1994, Narasimha and Brown, 2004, Ahmed et al., 2003, Casso et al., 2000, Rusten et al., 2001, Li and Vaessin, 2000, Lorenzen et al., 2001, Martin-Bermudo and Brown, 2000, Seher and Leptin, 2000, Gao et al., 2000, Roman et al., 2000, Ingham et al., 2000, Zaffran and Frasch, 2000, Leclerc et al., 2000, Gao et al., 1999, Casso et al., 1999, Holmes and Heilig, 1999, Hipfner et al., 2004, Golembo et al., 1999, Yarnitzky et al., 1998, Gabay et al., 1997, Carhan et al., 2004, Ohshiro and Saigo, 1997, Shandala et al., 2004, Chu et al., 1998, Furlong et al., 2001, Schweitzer et al., 1995, Kuhnlein and Schuh, 1996, Yagi et al., 1998, Pielage et al., 2003, Correia et al., 2003, Giesen et al., 1997, Nakao and Campos-Ortega, 1996, Giebel et al., 1997, Jenkins et al., 2003, Yagi and Hayashi, 1997, Golembo et al., 1996, Gabay et al., 1996, Koizumi et al., 2003, Schnepp et al., 1998, Terry et al., 2006, Reddy et al., 2006, Altenhein et al., 2006, Herranz et al., 2006, Sweeney et al., 2006, Sweeney et al., 2006, Schaerlinger et al., 2007, Lovegrove et al., 2006, Li et al., 2004, Joly et al., 2007, Nanda et al., 2009, Rousset et al., 2010, Von Ohlen et al., 2007, Von Ohlen and Moses, 2009, Satoh et al., 2008, Helman et al., 2011, Leonardi et al., 2011, Moses et al., 2011, Mohseni et al., 2009)
Molecular data	Kr promoter drives expression of Scer\GAL4. (Schweitzer et al., 1995) Construct: Sequences encoding Scer\GAL4 were inserted downstream of a 5.0kb Kr control region. (Castelli-Gair et al., 1994)
Phenotypic class	lethal, with spi^Scer\UAS.cSa (Schweitzer et al., 1995) lethal, with vnd^Scer\UAS.cKa (Koizumi et al., 2003) lethal, with vnd^{Y54M.Scer\UAS} (Koizumi et al., 2003) lethal, with vnd^{Δacidic.Scer\UAS} (Koizumi et al., 2003) lethal, with vnd^{ΔNK-2box.Scer\UAS} (Koizumi et al., 2003) lethal, with vnd^{Δtinman.Scer\UAS} (Koizumi et al., 2003) lethal \| embryonic stage, with vnd^Scer\UAS.cMa (Mellerick and Modica, 2002) neuroanatomy defective, with brk^Scer\UAS.cJa (Rusten et al., 2002) neuroanatomy defective, with N^{int.G.Scer\UAS} (Ahmed et al., 2003) partially lethal - majority live, with dlt^Scer\UAS.cPa (Pielage et al., 2003) viable, with 5-HT2^Scer\UAS.cCa (Colas et al., 1999) viable \| poor \| heat sensitive, with 5-HT2^Scer\UAS.cCa (Colas et al., 1999) wild-type, with trbl^EP3519 (Seher and Leptin, 2000)
Phenotype manifest in	abdominal 1 lch5 neuron (Rusten et al., 2001) abdominal 2 lch5 neuron (Rusten et al., 2001) abdominal 3 lch5 neuron (Rusten et al., 2001) aCC neuron, with vnd^Scer\UAS.cCa (Chu et al., 1998) amnioserosa, with Atg1^Scer\UAS.cSa, Scer\GAL4^{Orct2-cald-GAL4} (Mohseni et al., 2009) amnioserosa, with Scer\GAL4^{Orct2-cald-GAL4}, W^GD1673 (Mohseni et al., 2009) Bolwig organ, with Fas2^Scer\UAS.cHa (Holmes and Heilig, 1999) CNS glial cell, with vnd^Scer\UAS.cMa (Mellerick and Modica, 2002) commissure, with vnd^Scer\UAS.cMa (Mellerick and Modica, 2002) dorsal abdominal cluster, with brk^Scer\UAS.cJa (Rusten et al., 2002) embryo, with Cdk7^{D137R.Scer\UAS.T:Ivir\HA1} (Leclerc et al., 2000) embryo, with Cdk7^{T170A.Scer\UAS.T:Ivir\HA1} (Leclerc et al., 2000) embryonic/larval neuron, with N^{int.G.Scer\UAS} (Ahmed et al., 2003) embryonic/larval posterior spiracle, with Abd-B^Scer\UAS.cCa (Castelli-Gair et al., 1994) embryonic/larval somatic muscle, with vn^Scer\UAS.cYa (Yarnitzky et al., 1998) embryonic epidermis, with pros^Scer\UAS.cMa (Li and Vaessin, 2000) embryonic epidermis, with spi^s.Scer\UAS (Schweitzer et al., 1995) extended germ band embryo, with Rho1^N19.Scer\UAS (Schock and Perrimon, 2002) extended germ band embryo, with Rho1^V12.Scer\UAS (Schock and Perrimon, 2002) fascicle, with vnd^Scer\UAS.cMa (Mellerick and Modica, 2002) filzkorper \| ectopic, with Abd-B^Scer\UAS.cCa (Castelli-Gair et al., 1994) gastrula embryo, with 5-HT2^Scer\UAS.cCa (Colas et al., 1999) germ band, with Pi3K92E^{D954A.Scer\UAS.T:Hsap\MYC} (Herranz et al., 2006) interface glial cell, with ttk^69.Scer\UAS (Badenhorst, 2001) Keilin's organ, with Ubx^Scer\UAS.cCa (Castelli-Gair et al., 1994) lateral abdominal cluster, with brk^Scer\UAS.cJa (Rusten et al., 2002) mesoderm, with pros^Scer\UAS.cMa (Li and Vaessin, 2000) mitotic cell cycle \| embryonic stage 1, with Cdk7^{D137R.Scer\UAS.T:Ivir\HA1} (Leclerc et al., 2000) mitotic cell cycle \| embryonic stage 1, with Cdk7^{T170A.Scer\UAS.T:Ivir\HA1} (Leclerc et al., 2000) pCC neuron, with vnd^Scer\UAS.cCa (Chu et al., 1998) peripheral nervous system, with ttk^p69.Scer\UAS (Giesen et al., 1997) peripheral nervous system, with ttk^p88.Scer\UAS (Giesen et al., 1997) photoreceptor \| embryonic stage, with Fas2^Scer\UAS.cHa (Holmes and Heilig, 1999) presumptive embryonic/larval peripheral nervous system, with brk^Scer\UAS.cJa (Rusten et al., 2002) RP2 motor neuron, with vnd^Scer\UAS.cCa (Chu et al., 1998) spiracular hair \| ectopic, with Abd-B^Scer\UAS.cCa (Castelli-Gair et al., 1994) tracheal primordium, with salm^Scer\UAS.cKa (Kuhnlein and Schuh, 1996) tracheal system, with salm^Scer\UAS.cKa (Kuhnlein and Schuh, 1996) U neuron, with vnd^Scer\UAS.cCa (Chu et al., 1998) ventral nerve cord, with pros^Scer\UAS.cMa (Li and Vaessin, 2000) ventral nerve cord, with vnd^Scer\UAS.cMa (Mellerick and Modica, 2002) ventral nerve cord primordium, with ttk^69.Scer\UAS (Badenhorst, 2001)
Other information

Allele	w^+mC
Reference(s)	(Castelli-Gair et al., 1994)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS) Stage Tissue/Position (including subcellular localization) Reference embryonic stage ectoderm (Golembo et al., 1996) parasegment 4 -- parasegment 9 (Schweitzer et al., 1995) embryonic stage 6 -- 10 parasegment 5 -- parasegment 9 (Castelli-Gair et al., 1994) embryonic stage 9 -- 12 amnioserosa (Casso et al., 2000) posterior ectoderm (Casso et al., 2000) embryonic stage 13 -- 15 amnioserosa (Narasimha and Brown, 2004) embryonic stage 16 -- 17 macrophage (Casso et al., 2000) Bolwig organ (Casso et al., 2000) embryonic stage 17 embryonic/larval posterior spiracle (Casso et al., 2000) first instar larval stage Bolwig organ (Casso et al., 2000) embryonic/larval posterior spiracle (Casso et al., 2000) second instar larval stage embryonic/larval salivary gland (Casso et al., 2000) third instar larval stage embryonic/larval fat body (Casso et al., 2000) pupal stage \| early embryonic/larval fat body (Casso et al., 2000) pupal stage \| late pupal abdomen (Casso et al., 2000) adult stage adult abdomen \| restricted (Casso et al., 2000)
Additional Information
Statement Reference In embryos, expression easily scored at late extended germ band; fades during germ band retraction (stage 13-14), increases at end of embryogenesis, where it is visible in Bolwig's organ and the posterior spiracle. In larva, first instar pattern similar to that in late embryo; in second instar most conspicuous expression in the salivary glands; in late third instar and early pupae, in the fat body. In late pupae and young adults, signal observed internally throughout abdomen; in older adults restricted to internal abdominal structures near the thorax. (Casso et al., 2000) (Described as expressed in parasegments T2 to A4.) (Schweitzer et al., 1995) In embryos, no expression observed in the CNS. (Castelli-Gair et al., 1994)

Marker for	amnioserosa (Narasimha and Brown, 2004)
Reflects expression of	Kr (Castelli-Gair et al., 1994, Casso et al., 2000)
Progenitors & Descendants
progenitor(s)	pP{GAL4-Kr.C} (Castelli-Gair et al., 1994)
descendant(s)
Comments

Stocks Listed in FlyBase ( 110 )
Bloomington	9399 Pvr⁵³⁶³/CyO, P{GAL4-Kr.C}DC3, P{UAS-GFP.S65T}DC7 7155 w^; Df(2R)247/CyO, P{GAL4-Kr.C}DC3, P{UAS-GFP.S65T}DC7 5193 Df(1)JA27/FM7c, P{GAL4-Kr.C}DC1, P{UAS-GFP.S65T}DC5, sn⁺ 7294 Df(3L)4231/TM3, P{GAL4-Kr.C}DC2, P{UAS-GFP.S65T}DC10, Sb¹ 8475 w^; Df(2R)Nipped-D/CyO, P{GAL4-Kr.C}DC3, P{UAS-GFP.S65T}DC7 25901 w^; ncm^2a1/CyO, P{GAL4-Kr.C}DC3, P{UAS-GFP.S65T}DC7 25909 w^; ncm^2a2/CyO, P{GAL4-Kr.C}DC3, P{UAS-GFP.S65T}DC7 34048 w^; P{UAS-Roc1b.D}1/CyO, P{GAL4-Kr.C}DC3, P{UAS-GFP.S65T}DC7 25905 w^; Prp8^2e1/CyO, P{GAL4-Kr.C}DC3, P{UAS-GFP.S65T}DC7 25907 w^; RpL5^2d2/CyO, P{GAL4-Kr.C}DC3, P{UAS-GFP.S65T}DC7 25910 w^; RpL5^2d1/CyO, P{GAL4-Kr.C}DC3, P{UAS-GFP.S65T}DC7
Kyoto	108095 Df(1)JA27/FM7c, P{GAL4-Kr.C}DC1, P{UAS-GFP.S65T}DC5, sn⁺
	More stocks available...
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	Gal4^Kr (Satoh et al., 2008) Kr^GAL4 (Hipfner et al., 2004) Kr-Gal4 (Shandala et al., 2004, Correia et al., 2003, Rusten et al., 2002, Schock and Perrimon, 2002, Liu et al., 2002, Inbal et al., 2001, Badenhorst, 2001, Colas et al., 1999, Golembo et al., 1999, Reddy et al., 2006, Herranz et al., 2006, Schaerlinger et al., 2007, Lovegrove et al., 2006, Helman et al., 2011, Moses et al., 2011) Kr-GAL4 (Donaldson et al., 2004, Ahmed et al., 2003, Fung et al., 2003, Koizumi et al., 2003, Schock and Perrimon, 2003, Murthy et al., 2003, Rusten et al., 2001, Ingham et al., 2000, Li and Vaessin, 2000, Zaffran and Frasch, 2000, Holmes and Heilig, 1999, Castelli-Gair et al., 1994, Schweitzer et al., 1995, Terry et al., 2006, Rousset et al., 2010, Leonardi et al., 2011) Krgal4 (Hooper, 2003) KrGal4 (Shandala et al., 2003, Von Ohlen and Moses, 2009) KrGAL4 (Overton et al., 2002, Lorenzen et al., 2001, Mohseni et al., 2009) kr-GAL4 (Leclerc et al., 2000) KrGFP (Kiger et al., 2001) kruppel (Joly et al., 2007) Kruppel::GAL4 (Carhan et al., 2004) Kruppel::Gal4 (Narasimha and Brown, 2004, Narasimha and Brown, 2004) Kruppel-Gal4 (Jenkins et al., 2003, Sharrow and Tiemeyer, 2001, Wessells et al., 1999, Gao et al., 1999, Herranz et al., 2006, Sweeney et al., 2006, Sweeney et al., 2006, Li et al., 2004) Kruppel Gal4 (Gao et al., 2000, Von Ohlen et al., 2007) kruppel-GAL4 (Martin-Bermudo and Brown, 2000) Kruppel-GAL4 (Seher and Leptin, 2000) kruppel-gal4 (Yarnitzky et al., 1998) P{GAL4-Kr.C} (FlyBase, 1996-, Mohseni et al., 2009) P{w^+mC=Kr-GAL4} (Casso et al., 2000, Casso et al., 1999) P^Gal4-Kr.C (Nanda et al., 2009)
Secondary FlyBase IDs
	FBtp0000731
References ( 79 )

Generate a list of	All references relating to this transgenic transposon ( 79 )

List References by type	Research paper ( 76 ) Supplementary material ( 2 ) FlyBase analysis ( 1 )
Recent research papers ( 3 )
	Helman et al., 2011, Curr. Biol. 21(13): 1102--1110 Phosphorylation of Groucho Mediates RTK Feedback Inhibition and Prolonged Pathway Target Gene Expression. [FBrf0214255] Leonardi et al., 2011, Development 138(16): 3569--3578 Multiple O-glucosylation sites on Notch function as a buffer against temperature-dependent loss of signaling. [FBrf0214548] Moses et al., 2011, Dev. Biol. 360(1): 208--215 Phosphorylation of Ind by MAP kinase enhances Ind-dependent transcriptional repression. [FBrf0216621] Show all research papers ...