Recombinant construct P{RS5}

General Information
Symbol	P{RS5}	FlyBase ID	FBtp0001532
Feature type	transgenic_transposon

Size	5.975	Expression data
Associated insertions	1691 available
Molecular map
Recent Updates
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FB2013_03	Stocks FBst0043649
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Description & Uses
Species	synthetic construct
Location-dependent role	carries FRT site(s) (Golic and Golic, 1996) deletion generation component (Golic and Golic, 1996)
Description	CV term Qualifiers & info Reference transposon P-element (Golic and Golic, 1996, Ryder et al., 2004)
Uses	CV term Qualifiers & info Reference genomic insertion screen mutagenesis chromosomal aberrations (Golic and Golic, 1996, Ryder et al., 2004)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size	5.975
Left end
Right end

Segments	Number Orientation Symbol 1 + Car1_P5'(P5',R1) 2 + 001378 3 + FRT_200bp 4 + 001379 5 - RS3.RS5.mW.FRT 6 - 001003 7 + Car1_P3'(H3,P3')
Reference	(Golic and Golic, 1996)
Features
	CV term Qualifiers & info Reference chimeric gene (Golic and Golic, 1996) removable element (Golic and Golic, 1996, Ahmad and Golic, 1996, Golic and Golic, 1996) selectable marker (Golic and Golic, 1996)
Component Alleles
Allele	w^{+mW.Scer\FRT.hs}
Reference(s)	(Golic and Golic, 1996, Ryder et al., 2004, Golic and Golic, 1996, Ahmad and Golic, 1996)
Molecular data	Construct: Scer\FRT sequence is inserted between the exons of w^+mW.hs. (Ahmad and Golic, 1996) A single Scer\FRT is inserted into the first intron of w. (Golic and Golic, 1996) Scer\FRT site is present in the first intron of w^+mW.hs. (Golic and Golic, 1996)
Phenotypic class	eye color defective (Golic and Golic, 1996)
Phenotype manifest in	pigment cell (Golic and Golic, 1996, Golic and Golic, 1996)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	pP{RS5} (Golic and Golic, 1996)
descendant(s)	P{?'RS5} (Hödl and Basler, 2009) P{RS5r} (Golic and Golic, 1996, Ryder et al., 2004)
Comments
	Additional information at: DrosDel RS Elements http://www.drosdel.org.uk/ddelements.html. (Ryder et al., 2004) report comment: See report for Scer\FRT. (Ahmad and Golic, 1996)
Stocks Listed in FlyBase ( 1309 )
Kyoto	125007 w¹¹¹⁸ P{RS5}5-HA-1012 125011 P{RS5}5-HA-1019 w¹¹¹⁸ 125018 P{RS5}5-HA-1028 w¹¹¹⁸ 125019 w¹¹¹⁸ P{RS5}5-HA-1030 125020 w¹¹¹⁸ P{RS5}5-HA-1031 125030 w¹¹¹⁸ P{RS5}5-HA-1049 125045 w¹¹¹⁸ P{RS5}5-HA-1067 125111 w¹¹¹⁸ P{RS5}5-HA-1157 125124 w¹¹¹⁸ P{RS5}5-HA-1181 125233 w¹¹¹⁸ P{RS5}5-HA-1520 125236 w¹¹¹⁸ P{RS5}5-HA-1523 125245 w¹¹¹⁸ P{RS5}5-HA-1537
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External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	P[RS5] (Golic and Golic, 1996, Ahmad and Golic, 1998) P{FRT(RS5)} (FlyBase, 1996-) P{RS5} (Ryder et al., 2004, FlyBase, 1996-)
Secondary FlyBase IDs

References ( 9 )
Research paper	Dearborn et al., 2012, PLoS ONE 7(5): e37303 Reph, a Regulator of Eph Receptor Expression in the Drosophila melanogaster Optic Lobe. [FBrf0218347] Hödl and Basler, 2009, Curr. Biol. 19(14): 1221--1226 Transcription in the absence of histone H3.3. [FBrf0208395] Phalke et al., 2009, Nat. Genet. 41(6): 696--702 Retrotransposon silencing and telomere integrity in somatic cells of Drosophila depends on the cytosine-5 methyltransferase DNMT2. [FBrf0208049] Ryder et al., 2004, Genetics 167(2): 797--813 The DrosDel collection: a set of P-element insertions for generating custom chromosomal aberrations in Drosophila melanogaster. [FBrf0179424] Ahmad and Golic, 1998, Genetics 148(2): 775--792 The transmission of fragmented chromosomes in Drosophila melanogaster. [FBrf0101865] Ahmad and Golic, 1996, Genetics 144(2): 657--670 Somatic reversion of chromosomal position effects in Drosophila melanogaster. [FBrf0090428] Golic and Golic, 1996, Genetics 144(4): 1693--1711 Engineering the Drosophila genome: chromosome rearrangements by design. [FBrf0091061] Golic and Golic, 1996, Genetics 143(1): 385--400 A quantitative measure of the mitotic pairing alleles in Drosophila melanogaster and the influence of structural heterozygosity. [FBrf0087226]
FlyBase analysis	FlyBase Curators, 2008, Insertion identifiers and alleles based on genomic location of insertions with respect to gene annotations. Insertion identifiers and alleles based on genomic location of insertions with respect to gene annotations. [FBrf0202955]