Recombinant construct P{RS3}

General Information
Symbol	P{RS3}	FlyBase ID	FBtp0001534
Feature type	transgenic_transposon

Size	6.046	Expression data
Associated insertions	1698 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role	carries FRT site(s) (Golic and Golic, 1996) deletion generation component (Golic and Golic, 1996)
Description	CV term Qualifiers & info Reference transposon P-element (Golic and Golic, 1996, Ryder et al., 2004)
Uses	CV term Qualifiers & info Reference genomic insertion screen mutagenesis chromosomal aberrations (Golic and Golic, 1996, Ryder et al., 2004)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size	6.046
Left end
Right end

Segments	Number Orientation Symbol 1 + Car1_P5'(P5',R1) 2 + 001380 3 - FRT_200bp 4 + 001381 5 + RS3.RS5.mW.FRT 6 + ^EcoRIef/^HindIIIef 7 + Car1_P3'(H3,P3')
Reference	(Golic and Golic, 1996)
Features
	CV term Qualifiers & info Reference chimeric gene (Golic and Golic, 1996) removable element (Ahmad and Golic, 1996, Golic and Golic, 1996, Martin-Morris et al., 1997) selectable marker (Golic and Golic, 1996)
Component Alleles
Allele	w^{+mW.Scer\FRT.hs}
Reference(s)	(Golic and Golic, 1996, Martin-Morris et al., 1997, Ryder et al., 2004, Ahmad and Golic, 1996)
Molecular data	Construct: Scer\FRT sequence is inserted between the exons of w^+mW.hs. (Ahmad and Golic, 1996) A single Scer\FRT is inserted into the first intron of w. (Golic and Golic, 1996) Scer\FRT site is present in the first intron of w^+mW.hs. (Golic and Golic, 1996)
Phenotypic class	eye color defective (Golic and Golic, 1996)
Phenotype manifest in	pigment cell (Golic and Golic, 1996, Golic and Golic, 1996)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	pP{RS3} (Golic and Golic, 1996)
descendant(s)	P{?RS3} (Bergner et al., 2010) P{RS3r} (Golic and Golic, 1996, Ryder et al., 2004)
Comments
	Additional information at: DrosDel RS Elements http://www.drosdel.org.uk/ddelements.html. (Ryder et al., 2004) report comment: See report for Scer\FRT. (Ahmad and Golic, 1996)
Stocks Listed in FlyBase ( 1491 )
Kyoto	123010 w¹¹¹⁸ P{RS3}CB-0019-3 123028 w¹¹¹⁸ P{RS3}CB-0084-3 123035 w¹¹¹⁸ P{RS3}CB-0106-3 123072 w¹¹¹⁸ P{RS3}CB-0206-3 123102 w¹¹¹⁸ P{RS3}CB-0274-3 123130 w¹¹¹⁸ P{RS3}CB-0332-3 123144 w¹¹¹⁸ P{RS3}CB-0360-3 123154 w¹¹¹⁸ P{RS3}CB-0392-3 123156 w¹¹¹⁸ P{RS3}CB-0402-3 123164 w¹¹¹⁸ P{RS3}CB-0423-3 123193 w¹¹¹⁸ P{RS3}CB-0477-3 123221 w¹¹¹⁸ P{RS3}CB-0526-3
	More stocks available...
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	P[RS3] (Golic and Golic, 1996) P{FRT(RS3)} (FlyBase, 1996-) P{RS3} (FlyBase, 1996-, Ryder et al., 2004)
Secondary FlyBase IDs

References ( 8 )
Research paper	Bergner et al., 2010, DNA Cell Biol. 29(9): 487--498 A novel predicted bromodomain-related protein affects coordination between meiosis and spermiogenesis in Drosophila and is required for male meiotic cytokinesis. [FBrf0211706] Dansereau and Lasko, 2008, J. Cell Biol. 182(5): 963--977 RanBPM regulates cell shape, arrangement, and capacity of the female germline stem cell niche in Drosophila melanogaster. [FBrf0206912] Ryder et al., 2004, Genetics 167(2): 797--813 The DrosDel collection: a set of P-element insertions for generating custom chromosomal aberrations in Drosophila melanogaster. [FBrf0179424] Martin-Morris et al., 1997, Genetics 147(2): 671--677 Heterochromatic trans-inactivation of Drosophila white transgenes. [FBrf0098841] Ahmad and Golic, 1996, Genetics 144(2): 657--670 Somatic reversion of chromosomal position effects in Drosophila melanogaster. [FBrf0090428] Golic and Golic, 1996, Genetics 144(4): 1693--1711 Engineering the Drosophila genome: chromosome rearrangements by design. [FBrf0091061]
FlyBase analysis	FlyBase Curators, 2008, Insertion identifiers and alleles based on genomic location of insertions with respect to gene annotations. Insertion identifiers and alleles based on genomic location of insertions with respect to gene annotations. [FBrf0202955] FlyBase, 2007.6, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0199566]