Recombinant construct P{UAS-Khc::lacZ}

General Information
Symbol	P{UAS-Khc::lacZ}	FlyBase ID	FBtp0001539
Feature type	transgenic_transposon

Size		Expression data	lacZ reporter
Associated insertions	2 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Clark et al., 1997)
Uses	CV term Qualifiers & info Reference binary system (targeted) Khc (Clark et al., 1997) Khc \| Ecol\lacZ (Clark et al., 1997) characterization Khc (Clark et al., 1997) reporter construct Khc (Clark et al., 1997)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference binary component (Clark et al., 1997) chimeric gene (Clark et al., 1997) molecular tag (Clark et al., 1997) selectable marker (Clark et al., 1997)
Component Alleles
Allele	Khc^{Scer\UAS.T:Ecol\lacZ}
Reference(s)	(Clark et al., 1997, Zheng et al., 2008, Jankovics et al., 2002, Myat and Andrew, 2002, Benton et al., 2002, Mosley-Bishop et al., 1999, Ito et al., 1995, Kraft et al., 2006, Bitan et al., 2010, Klusza and Deng, 2010, Song et al., 2012)
Molecular data	Construct: Expression of the Khc^T:Ecol\lacZ coding region is governed by Scer\UAS regulatory sequences. (Clark et al., 1997)
Phenotypic class	wild-type, with Scer\GAL4^hs.2sev (Mosley-Bishop et al., 1999)
Phenotype manifest in
Other information

Allele	w^+mC
Reference(s)	(Clark et al., 1997)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS) Stage Tissue/Position (including subcellular localization) Reference third instar larval stage photoreceptor cell \| apical (Mosley-Bishop et al., 1999)
Additional Information
Statement Reference This construct is a marker for plus ends of microtubules, and localizes apically in developing facets. (Mosley-Bishop et al., 1999)

Marker for	microtubule (Mosley-Bishop et al., 1999)
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	Kin::Β-Gal (Klusza and Deng, 2010) Kin:LacZ (Benton et al., 2002) Kin:β-Gal (Jankovics et al., 2002) KZ32 (Benton et al., 2002) P{UAS-Khc::lacZ} (FlyBase, 1996-) pUAS KHC-&Bgr-Gal (Bitan et al., 2010) UAS-Khc-lacZ (Kraft et al., 2006) UAS-kinesinLacZ (Myat and Andrew, 2002) UAS-kin-lacZ (Zheng et al., 2008, Song et al., 2012) UAS-KZ (Clark et al., 1997, Mosley-Bishop et al., 1999)
Secondary FlyBase IDs

References ( 11 )
Research paper	Song et al., 2012, Genes Dev. 26(14): 1612--1625 Regeneration of Drosophila sensory neuron axons and dendrites is regulated by the Akt pathway involving Pten and microRNA bantam. [FBrf0218927] Bitan et al., 2010, Mol. Cell. Biol. 30(2): 496--507 Asymmetric microtubule function is an essential requirement for polarized organization of the Drosophila bristle. [FBrf0209571] Klusza and Deng, 2010, Fly 4(2): 128--136 poly is required for nurse-cell chromosome dispersal and oocyte polarity in Drosophila. [FBrf0211064] Zheng et al., 2008, Nat. Cell Biol. 10(10): 1172--1180 Dynein is required for polarized dendritic transport and uniform microtubule orientation in axons. [FBrf0206898] Kraft et al., 2006, J. Neurosci. 26(34): 8734--8747 Phenotypes of Drosophila brain neurons in primary culture reveal a role for fascin in neurite shape and trajectory. [FBrf0191992] Benton et al., 2002, Dev. Cell 3(5): 659--671 Drosophila 14-3-3/PAR-5 is an essential mediator of PAR-1 function in axis formation. [FBrf0151902] Jankovics et al., 2002, Curr. Biol. 12(23): 2060--2065 MOESIN crosslinks actin and cell membrane in Drosophila oocytes and is required for OSKAR anchoring. [FBrf0155622] Myat and Andrew, 2002, Cell 111(6): 879--891 Epithelial tube morphology is determined by the polarized growth and delivery of apical membrane. [FBrf0155504] Mosley-Bishop et al., 1999, Curr. Biol. 9(21): 1211--1220 Molecular analysis of the klarsicht gene and its role in nuclear migration within differentiating cells of the Drosophila eye. [FBrf0111985] Clark et al., 1997, Development 124(2): 461--470 Reciprocal localization of Nod and kinesin fusion proteins indicates microtubule polarity in the Drosophila oocyte, epithelium, neuron and muscle. [FBrf0092501] Ito et al., 1995, Rouxs Arch. Dev. Biol. 204(5): 284--307 Distribution, classification, and development of Drosophila glial cells in the late embryonic and early larval ventral nerve cord. [FBrf0082171]