Recombinant construct P{ActGFP}

General Information
Symbol	P{ActGFP}	FlyBase ID	FBtp0001557
Feature type	transgenic_transposon

Size		Expression data	GFP reporter
Associated insertions	5 available
Molecular map
Recent Updates
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FB2013_03	Stocks FBst0043644 FBst0043643
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Reichhart and Ferrandon, 1998.6.8)
Uses	CV term Qualifiers & info Reference reporter construct Act5C (Reichhart and Ferrandon, 1998.6.8) vital (Reichhart and Ferrandon, 1998.6.8)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference chimeric gene (Reichhart and Ferrandon, 1998.6.8) reporter gene (Reichhart and Ferrandon, 1998.6.8) selectable marker (Reichhart and Ferrandon, 1998.6.8, Reichhart and Ferrandon, 1998)
Component Alleles
Allele	Avic\GFP^Act5C.PR
Reference(s)	(Bogwitz et al., 2005, Jung et al., 2005, Walters et al., 2005, Sarkar and Lakhotia, 2005, Genova and Fehon, 2003, Narasimha and Brown, 2004, Narasimha and Brown, 2004, Mason et al., 2003, Peyre and Aigaki, 1999, Freilich et al., 1999, Qi et al., 2004, Donaldson et al., 2004, Perrin et al., 2004, Reichhart and Ferrandon, 1998, Reichhart and Ferrandon, 1998.6.8, Katsuyama et al., 2005, Smith and Prehoda, 2011, Metzstein and Krasnow, 2006)
Molecular data	Construct: Distal Act5C promoter drives expression of the S65T version of Avic\GFP coding region, followed by the Drs terminator. (Reichhart and Ferrandon, 1998.6.8)
Phenotypic class
Phenotype manifest in
Other information

Allele	w^+mC
Reference(s)	(Reichhart and Ferrandon, 1998.6.8, Reichhart and Ferrandon, 1998)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression
distribution deduced from reporter Stage Tissue/Position (including subcellular localization) Reference embryonic stage yolk (Reichhart and Ferrandon, 1998.6.8) embryonic stage 15 -- 17 embryonic/larval midgut (Reichhart and Ferrandon, 1998.6.8) first instar larval stage embryonic/larval salivary gland duct (Reichhart and Ferrandon, 1998.6.8) third instar larval stage midgut muscle (Reichhart and Ferrandon, 1998.6.8) embryonic/larval proventriculus (Reichhart and Ferrandon, 1998.6.8) cuprophilic cell (Reichhart and Ferrandon, 1998.6.8) imaginal disc (Reichhart and Ferrandon, 1998.6.8) adult stage eye (Reichhart and Ferrandon, 1998.6.8) adult abdomen (Reichhart and Ferrandon, 1998.6.8) ventriculus circular muscle (Reichhart and Ferrandon, 1998.6.8) proboscis (Reichhart and Ferrandon, 1998.6.8) adult stage \| female seminal receptacle (Reichhart and Ferrandon, 1998.6.8) oogenesis & adult stage \| female ovarian sheath (Reichhart and Ferrandon, 1998.6.8) spermatogenesis & adult stage male reproductive system (Reichhart and Ferrandon, 1998.6.8) oogenesis (Reichhart and Ferrandon, 1998.6.8)
Additional Information
Statement Reference In the embryo, there is a strong maternal contribution which masks the zygotic expression until about stage 15, when a weak signal can be detected in the embryonic midgut. Since their cuticle is transparent, third instar larvae are easy to score under the fluorescent dissecting microscope. The main GFP expression pattern consists of a strong fluorescence in the salivary duct, the copper cells, the proventriculus and the visceral musculature of the midgut. A weaker signal can be detected in imaginal disks. In first instar larvae, the fluorescence appears to be restricted to the midgut. GFP expression observed in the reproductive tract of the male; in females, in ovaries (yolk of mature stages and musculature of the ovary sheath) and in the seminal receptacle. In many animals, the visceral musculature of the midgut is also fluorescent. (Reichhart and Ferrandon, 1998.6.8)

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	pP{ActGFP} (Reichhart and Ferrandon, 1998.6.8)
descendant(s)	P{ActGFP.w^-} (Carre and Antoniewski, 2005.10)
Comments

Stocks Listed in FlyBase ( 88 )
Bloomington	9562 mud¹/FM7i, P{ActGFP}JMR3 9563 mud⁴/FM7i, P{ActGFP}JMR3 38390 Pabp2⁵⁵/CyO, P{ActGFP}JMR1 36281 Tango11²/CyO, P{ActGFP}JMR1 36320 Tango11¹/CyO, P{ActGFP}JMR1 6590 Ptp52F^18.3/CyO, P{ActGFP}JMR1 4759 pan²/P{ActGFP}unc-13^GJ 6589 Ptp52F^8.10.3/CyO, P{ActGFP}JMR1 6665 w^* rok¹/FM7i, P{ActGFP}JMR3 9372 w^* sd¹¹/FM7i, P{ActGFP}JMR3 23693 w^* flw⁶/FM7i, P{ActGFP}JMR3 30560 Df(1)MH30, w¹¹¹⁸/FM7i, P{ActGFP}JMR3
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External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	ACT:GFP (Metzstein and Krasnow, 2006) Act-GFP (Bogwitz et al., 2005, Donaldson et al., 2004, Perrin et al., 2004, Katsuyama et al., 2005) actin5c::GFP (Narasimha and Brown, 2004, Narasimha and Brown, 2004) actin5C-GFP (Jung et al., 2005) Actin-GFP (Smith and Prehoda, 2011) P[pActGFP] (Reichhart and Ferrandon, 1998.6.8) P{ActGFP} (FlyBase, 1996-) P{w+ Act5C-GFP} (Walters et al., 2005) P{w+mC=ActGFP} (Genova and Fehon, 2003)
Secondary FlyBase IDs

References ( 21 )

Generate a list of	All references relating to this transgenic transposon ( 21 )

List References by type	Research paper ( 15 ) Supplementary material ( 2 ) Personal communication to FlyBase ( 4 )
Recent research papers ( 1 )
	Smith and Prehoda, 2011, Mol. Cell 43(4): 540--549 Robust spindle alignment in Drosophila neuroblasts by ultrasensitive activation of pins. [FBrf0214708] Show all research papers ...