Recombinant construct P{SUPor-P}

General Information
Symbol	P{SUPor-P}	FlyBase ID	FBtp0001587
Feature type	transgenic_transposon	Created / Updated	1998-09-30/1999-05-13

Size	11.467	Expression data
Location-dependent role		Associated insertions	2492 available
Molecular map
Description & Uses
Species	synthetic construct
Description	CV term Qualifiers & info Reference transposon P-element (Roseman et al., 1995, Parks et al., 2004, Bellen et al., 2004)
Uses	CV term Qualifiers & info Reference characterization gypsy\su(Hw)BR (Roseman et al., 1995) P-element mutagenesis (Roseman et al., 1995) genomic insertion screen lethal (Roseman et al., 1995)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)	FlyBase-compiled
Sequence
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size	11.467
Left end	P5\' (Roseman et al., 1993)
Right end	P3\' (Roseman et al., 1993)

Segments	Number Orientation Symbol 1 + Car1_P5'(P5',R1) 2 + 001327 3 - su(Hw)BR.0.43 4 + 001328 5 - w^BR.E.BR4.5 6 + 001329 7 - su(Hw)BR.0.43 8 + 001330 9 + y^+mDint25.2(S,S) 10 + 001326 11 + Car1_P3'(H3,P3')
Reference	(Roseman et al., 1993)
Features
	CV term Qualifiers & info Reference selectable marker (Patton et al., 1992, Roseman et al., 1995, Roseman et al., 1993, Mallin et al., 1998) selectable marker (Roseman et al., 1993) structural element (Roseman et al., 1995) regulatory/structural DNA (Roseman et al., 1993, Roseman et al., 1995, Mallin et al., 1998, Kurenova et al., 1998)
Component Alleles
y^+mDint2
Reference(s)	(Patton et al., 1992, Roseman et al., 1995, Donaldson et al., 2002, Maggert and Golic, 2002, Roseman et al., 1993, Yan et al., 2002, Roseman et al., 2001, Mallin et al., 1998)
Molecular data	5.2kb SalI y gene intronless fragment containing 2.9kb of 5' flanking and 0.3kb of 3' flanking DNA. (Patton et al., 1992) 5kb intronless SalI fragment containing the y gene. (Harrison and Perrimon, 1993) Construct: 5.2kb y⁺ gene including 2.8kb of 5' flanking sequences and 0.13kb of 3' sequences and lacks the tissue-specific bristle and tarsal claw enhancers. (Roseman et al., 1995) 5.2kb y gene fragment containing 2.8kb of 5' flanking and 0.13kb of 3' flanking DNA and lacking the intron that is the location of the tissue-specific bristle and tarsal claw enhancers. (Roseman et al., 1995)
Phenotypic class	body color defective (Ring and Garza, 2003.6.10, Roseman et al., 2001) visible (Roseman et al., 2001)
Phenotype manifest in	macrochaeta (Ring and Garza, 2003.6.10) unguis (Ring and Garza, 2003.6.10) arista (Ring and Garza, 2003.6.10) adult cuticle (Roseman et al., 2001)
Other information
w^BR.E.BR
Reference(s)	(Roseman et al., 1993, Roseman et al., 1995, Maggert and Golic, 2002, Lankenau et al., 2000, Kurenova et al., 1998, Bhadra et al., 1999, Soldatov et al., 1999, Roseman et al., 2001, Mallin et al., 1998)
Molecular data	Construct: w^+mC minigene altered to include a su(Hw) binding region (all 12 binding sites, nucleotides 647 to 1077 of Marlor et al., Mol Cell Biol 6:1129-1134), upstream of the w eye enhancer (nucleotides -1084 to -1465 of Qian et al., Genetics 131:79-90), upstream of the w coding region, upstream of a second su(Hw) binding region. (Roseman et al., 1993) Two gypsy\su(Hw)BR binding sites flank the w^+mET sequences to insulate the w gene from chromosomal position effects. (Roseman et al., 1995) P{SUPor-P-lt} contains a mini-w gene that has two flanking gypsy\su(Hw)BR sites, one of which is upstream of the w eye promoter. (Bhadra et al., 1999)
Phenotypic class	visible \| recessive (Roseman et al., 1993) eye color defective (Kurenova et al., 1998, Roseman et al., 2001)
Phenotype manifest in	pigment cell (Roseman et al., 1993, Kurenova et al., 1998, Roseman et al., 2001)
Other information
gypsy\su(Hw)BR⁴³⁰
Reference(s)	(Roseman et al., 1995, Roseman et al., 1993, Lankenau et al., 2000, Kurenova et al., 1998, Mallin et al., 1998)
Molecular data	Construct: Consists of a 430bp gypsy\su(Hw)BR, including all 12 binding sites. (Patton et al., 1992) Consists of a 430bp gypsy\su(Hw)BR, including all 12 binding sites, nucleotides 647 to 1077 of FBrf0045014. (Roseman et al., 1993) Consists of a 430bp gypsy\su(Hw)BR, gypsy sequences 647-1077 (coordinates of FBrf0045014). (Roseman et al., 1995) Contains 12 su(Hw)-binding sites, corresponding to bp 647-1077 in the gypsy transposon. (Kuhn et al., 2003)
Phenotypic class
Phenotype manifest in
Other information
Expression Data
Reporter Expression
Stage Tissue/Position Reference
Additional Information
Statement Reference

Assay mode
Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	pP{CaSpeR-3} pP{CaSpW18} (Roseman et al., 1993, Roseman et al., 1993)
descendant(s)	P{5'SUPor-P} P{3'SUPor-P} (Dobie et al., 2001, Dobie et al., 2001) P{?SUPor-P} (FlyBase, 1992-)
Comments

Synonyms & Secondary IDs
Reported As
Symbol Synonym	B.R.:Eye>w>B.R. (Roseman et al., 1993) P(y⁺) (Donaldson et al., 2002) P{@gypsy\su(Hw)BR⁴³⁰@ @w^BR.E.BR@ @gypsy\su(Hw)BR⁴³⁰@ @y^+mDint2@=@SUPor-P@} (FlyBase, 1996-) P{SUPor-P} (FlyBase, 1996-, Parks et al., 2004, Dobie et al., 2001, Gene Disruption Project members, 2001-, Bellen et al., 2004) SUPor-P (Soldatov et al., 1999) SuPor-P-It (Bhadra et al., 1999)
Secondary FlyBase IDs

References ( 35 )

Generate a list of	All references relating to this transgenic transposon ( 35 )

List References by type	Research paper ( 28 ) Personal communication to FlyBase ( 3 ) FlyBase analysis ( 3 ) Computer file ( 1 )
Recent research papers ( 8 )
	Caldwell et al., 2007, Fly 1(3): 146--152 reduced ocelli Encodes the Leucine Rich Repeat Protein Pray For Elves in Drosophila melanogaster. [FBrf0200339] Pepple et al., 2007, Genetics 175(1): 125--141 A genetic screen in Drosophila for genes interacting with senseless during neuronal development identifies the importin moleskin. [FBrf0194654] Roy et al., 2007, Genetics 176(2): 801--813 Characterization of BEAF mutations isolated by homologous recombination in Drosophila. [FBrf0200670] Gilbert et al., 2006, Genetics 173(3): 1365--1375 The Drosophila boundary element-associated factors BEAF-32A and BEAF-32B affect chromatin structure. [FBrf0192350] Guelman et al., 2006, Molec. Cell. Biol. 26(19): 7178--7189 The essential gene wda encodes a WD40 repeat subunit of Drosophila SAGA required for histone H3 acetylation. [FBrf0192338] Jung et al., 2006, Curr. Biol. 16(12): 1224--1231 Polychaetoid/ZO-1 is required for cell specification and rearrangement during Drosophila tracheal morphogenesis. [FBrf0192782] Speder et al., 2006, Nature 440(7085): 803--807 Type ID unconventional myosin controls left-right asymmetry in Drosophila. [FBrf0191256] Wehr et al., 2006, Dev. Biol. 294(2): 406--417 Deadlock, a novel protein of Drosophila, is required for germline maintenance, fusome morphogenesis and axial patterning in oogenesis and associates with centrosomes in the early embryo. [FBrf0192692] Show all research papers ...