minigene altered to include a su(Hw)
binding region (all 12 binding sites, nucleotides 647 to 1077 of Marlor et al., Mol Cell Biol 6:1129-1134 ), upstream of the w
eye enhancer (nucleotides -1084 to -1465 of Qian et al., Genetics 131:79-90 ), upstream of the w
coding region, upstream of a second su(Hw)
binding sites flank the w+mET
sequences to insulate the w
gene from chromosomal position effects.