FlyBase Recombinant Construct Report: P{UAS-Pka-R1.BDK}

General Information

Symbol

P{UAS-Pka-R1.BDK}

FlyBase ID

FBtp0001595

Feature type

transgenic_transposable_element

Component Allele(s)

Pka-R1^BDK.UAS

Also Known As

UAS-PKAinh¹, UAS-R*, UAS-PKAr, UAS-PKAr*, UAS-PKA^DN, UAS-PKA-R1.BDK

Size

Expression Data

Associated insertion(s)

2 available

Molecular map

Description and Uses

Construct components

Component

Use(s)

Marker(s)

w^+mC

Component allele Pka-R1^BDK.UAS

Transgenic product class

dominant_negative_variant

(Wolfgang et al., 1996)

missense_variant

(Kalderon, 1998.3.12)

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Pka-R1

Description

UAS regulatory sequences drive expression of a mutant form of Pka-R1 that contains mutations in two cAMP binding sites (amino acid replacements G196E and G321D). This mutant regulatory subunit acts as an inhibitor of protein kinase A (PKA) activity; the mutations slightly reduce affinity for the catalytic subunit in vitro and makes the regulatory subunit entirely insensitive to cAMP.

(Kalderon, 1998.3.12)

Encodes a dominant negative regulatory form of Pka-R1.

(Wolfgang et al., 1996)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Phenotypic Data

Component Allele

Phenotypic Data

Pka-R1^BDK.UAS

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

pP{UAS-Pka-R1.BDK}

(Li et al., 1995)

descendant(s)

Comments

report comment: Pka-R1 allele in this construct is a mutant form which inhibits PKA function.

(Li et al., 1995)

Stocks (1)

Stocks containing insertion(s) of this recombinant construct

Bloomington

35550

w^*; P{UAS-Pka-R1.BDK}35

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (27)

Reported As

Symbol Synonym

PKA*

(Lee et al., 2009)

PKA-R1(BDK)

(Machado Almeida et al., 2021)

PKA^DN

(Hong et al., 2008)

P{UAS-Pka-R1.BDK}

(Kiger, 2011.5.13, FlyBase, 1996-)

P{UAS-R* w⁺}

(Alcedo et al., 2000)

UAS PKA-DN

(Melnattur et al., 2020)

UAS-PKA-DN

(Hong et al., 2012)

UAS-PKA-R*

(Thornquist et al., 2021)

UAS-PKA-R1.BDK

(Thornquist et al., 2021)

UAS-PKA-R1^BDK

(Kubrak et al., 2024)

UAS-PKA-ca

(Copf et al., 2019)

UAS-PKA^DN

(Kurogi et al., 2023, Kang et al., 2011, Hong et al., 2008)

UAS-PKA^inhibitor

(Hueston et al., 2016)

UAS-PKAR

(Wu et al., 2009)

UAS-PKAi

(Chen et al., 2008)

UAS-PKAinh

(Zhang et al., 2015)

UAS-PKAinh¹

(Kim et al., 2024, Baines, 2004, Mee et al., 2004, Baines, 2003)

UAS-PKAmR*

(Poernbacher and Vincent, 2018)

UAS-PKAr

(Guichard et al., 2006, Cho et al., 2001)

UAS-PKAr*

(Wolterhoff et al., 2020, Lee et al., 2009, Yang et al., 2009)

UAS-PKAr*BDK35

(Gonzales et al., 2016)

UAS-PKAr^*

(Kiger et al., 1999)

UAS-Pka-R1.BDK

(Kim et al., 2024)

UAS-R*

(Shi et al., 2014, Zhou et al., 2006, Jia et al., 2005, Zhang et al., 2005)

UAS-R^*

(Venkatesh et al., 2001)

UAS-pka-R1

(Baum et al., 2000)

constitutively active PKA

(Zhou and Kalderon, 2011)

Secondary FlyBase IDs

References (49)

Report Sections

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