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General Information
Symbol
P{UAS-Pka-R1.BDK}
FlyBase ID
FBtp0001595
Feature type
transgenic_transposable_element
Component Allele(s)
Also Known As
UAS-R*, UAS-PKAinh1, UAS-PKAr, UAS-PKAr*, UAS-PKADN
Size
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Location-dependent role
Description
CV term
Qualifiers and info
Reference
transposon
P-element
Construct components
Product class / Tool use(s)
Marker(s)
Component allele Pka-R1BDK.UAS
Product class / Tool use(s)
Description

UAS regulatory sequences drive expression of a mutant form of Pka-R1 that contains mutations in two cAMP binding sites (amino acid replacements G196E and G321D). This mutant regulatory subunit acts as an inhibitor of protein kinase A (PKA) activity; the mutations slightly reduce affinity for the catalytic subunit in vitro and makes the regulatory subunit entirely insensitive to cAMP.

Encodes a dominant negative regulatory form of Pka-R1.

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Features
CV term
Qualifiers and info
Reference
binary
component
chimeric
gene
Component Alleles
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
descendant(s)
Comments

report comment: Pka-R1 allele in this construct is a mutant form which inhibits PKA function.

Stocks (1)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (19)
References (41)