Recombinant construct P{UAS-FLP1.D}

General Information
Symbol	P{UAS-FLP1.D}	FlyBase ID	FBtp0001610
Feature type	transgenic_transposon

Size	7.725	Expression data
Associated insertions	2 available
Molecular map
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03	Stocks FBst0043658
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Duffy et al., 1998)
Uses	CV term Qualifiers & info Reference binary system (targeted) Scer\FLP1 (Duffy et al., 1998) characterization Scer\UAS (Duffy et al., 1998)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)	FlyBase-compiled
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size	7.725
Left end	P5\' (Duffy et al., 1998)
Right end	P3\' (Duffy et al., 1998)

Segments	Number Orientation Symbol 1 + CaSpeR_P5'(P5',Sp) 2 + 001187 3 - UAS::FLP1 4 + 001186 5 + Car1_P3'(H3,P3')
Reference	(Duffy et al., 1998)
Features
	CV term Qualifiers & info Reference binary component (Duffy et al., 1998) chimeric gene (Duffy et al., 1998) partial rescue (Pirrotta, 1988) selectable marker (Pirrotta, 1988, Duffy et al., 1998)
Component Alleles
Allele	Scer\FLP1^Scer\UAS.cDa
Reference(s)	(Duffy et al., 1998, Suzanne et al., 2001, Morrison and Halder, 2010, Singh and Choi, 2003, Mason et al., 2003, Pai et al., 2000, Collins and Treisman, 2000, Liu and Montell, 2001, Maurel-Zaffran and Treisman, 2000, Stowers and Schwarz, 1999, Liu and Montell, 1999, Tanentzapf et al., 2000, Benton and St. Johnston, 2003, Bazigou et al., 2007, Spasić et al., 2008, Evans et al., 2009, Evans et al., 2009, Pack-Chung et al., 2007, Herz et al., 2009, Potter et al., 2010, Viktorin et al., 2011, Wang et al., 2011, Ji and Tulin, 2012)
Molecular data	Construct: Expression of a SalI-XbaI fragment including the Scer\FLP1 coding region is governed by Scer\UAS regulatory sequences. (Duffy et al., 1998)
Phenotypic class
Phenotype manifest in
Other information

Allele	w^+mC
Reference(s)	(Pirrotta, 1988, Duffy et al., 1998)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	pP{UAST} (Duffy et al., 1998)
descendant(s)
Comments
	construct_comment: Progenitors are those of the plasmid, pP{UAS-FLP1.D}. (Duffy et al., 1998)
Stocks Listed in FlyBase ( 40 )
Bloomington	4540 w^; P{UAS-FLP1.D}JD2 4539 y¹ w^; P{UAS-FLP1.D}JD1 5075 P{FRT(w^hs)}G13; P{GawB}T155 P{UAS-FLP1.D}JD2 5082 y¹ w^; P{GawB}T155 P{UAS-FLP1.D}JD2 6356 y¹ w^; P{en2.4-GAL4}e16E P{UAS-FLP1.D}JD1 5083 y¹ w^*; P{en2.4-GAL4}e22c P{UAS-FLP1.D}JD1/CyO 5074 P{neoFRT}40A/CyO; P{GawB}T155 P{UAS-FLP1.D}JD2/TM3, Sb¹
Kyoto	107787 y¹ w^; P{UAS-FLP1.D}JD1 107788 w^; P{UAS-FLP1.D}JD2/TM3, Sb¹ 108021 P{FRT(w^hs)}G13; P{GawB}T155 P{UAS-FLP1.D}JD2 108029 y¹ w^; P{en2.4-GAL4}e22c P{UAS-FLP1.D}JD1/CyO 108751 y¹ w^; P{en2.4-GAL4}e16E P{UAS-FLP1.D}JD1/CyO
	More stocks available...
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{UAS-FLP1.D} (FlyBase, 1996-, Evans et al., 2009, Ji and Tulin, 2012) UAS>FLP (Tanentzapf et al., 2000) UAS-FLP (Bazigou et al., 2007, Pack-Chung et al., 2007, Potter et al., 2010) UAS-Flp (Morrison and Halder, 2010, Spasić et al., 2008) UAS FLP (Evans et al., 2009) UAS-flp (Viktorin et al., 2011) uas-flp (Wang et al., 2011) UASt FLP (Mason et al., 2003)
Secondary FlyBase IDs

References ( 23 )

Generate a list of	All references relating to this transgenic transposon ( 23 )

List References by type	Research paper ( 21 ) Supplementary material ( 2 )
Recent research papers ( 3 )
	Ji and Tulin, 2012, Nat. Commun. 3: 760 Poly(ADP-ribose) controls DE-cadherin-dependent stem cell maintenance and oocyte localization. [FBrf0217856] Viktorin et al., 2011, Dev. Biol. 356(2): 553--565 Multipotent neural stem cells generate glial cells of the central complex through transit amplifying intermediate progenitors in Drosophila brain development. [FBrf0214495] Wang et al., 2011, Proc. Natl. Acad. Sci. U.S.A. 108(27): 11139--11144 Sexually dimorphic regulation of the Wingless morphogen controls sex-specific segment number in Drosophila. [FBrf0214239] Show all research papers ...