A Database of Drosophila Genes & Genomes

FB2008_07, released August 8, 2008
 

Recombinant construct P{UAS-lacZ.NZ}

General Information
Symbol P{UAS-lacZ.NZ} FlyBase ID FBtp0001611
Feature type transgenic_transposon Created / Updated 1999-01-08/2000-02-03
Size Expression data lacZ reporter
Location-dependent role Associated insertions 4 available
Molecular map
hide Description & Uses
Species synthetic construct
Description
CV term
Qualifiers & info
Reference
transposon
P-element
(Bloomington Drosophila Stock Center, 1998.9.14)
Uses
CV term
Qualifiers & info
Reference
binary system (targeted)
Ecol\lacZ
(Bloomington Drosophila Stock Center, 1998.9.14)
reporter construct
Ecol\lacZ | binary system
(Bloomington Drosophila Stock Center, 1998.9.14)
Cloning Sites
Location
Restriction sites
Reference
hide Sequence Data
Sequence (FB)
Sequence
Associated Sequence Data
DDBJ /
EMBL /
GenBank
DNA sequence
Extent
 
hide Segments & Size
Total Size
Left end
Right end
Segments
Number
Orientation
Symbol
Reference
hide Features
CV term
Qualifiers & info
Reference
binary
component
(Bloomington Drosophila Stock Center, 1998.9.14)
chimeric
gene
(Bloomington Drosophila Stock Center, 1998.9.14)
selectable
marker
(Bloomington Drosophila Stock Center, 1998.9.14)
molecular
tag
(Bloomington Drosophila Stock Center, 1998.9.14)
reporter
gene
(Bloomington Drosophila Stock Center, 1998.9.14)
hide Component Alleles
hideEcol\lacZScer\UAS.T:SV40\nls2
Reference(s) (Bello et al., 2003, Brodu et al., 2002, Badenhorst, 2001, Yamada et al., 2003, Monier et al., 2005, Baker et al., 2003, Kozlova and Thummel, 2002, Cenci and Gould, 2005, Rangarajan et al., 1999, Baker et al., 2007, Lawrence et al., 2002, Takamatsu et al., 2002, Ward et al., 2002, Sen et al., 2004, Lee and Park, 2004, Astle et al., 2003, Dominguez et al., 2004, Shi and Skeath, 2004, Rival et al., 2004, Hatini et al., 2000, Bruckner et al., 2004, Jhaveri et al., 2000, Suzuki and Saigo, 2000, Wang et al., 2006, Garrity et al., 1999, Sato et al., 1999, Rival et al., 2004, Hatini and DiNardo, 2001, Bloomington Drosophila Stock Center, 1998.9.14, Kozlova and Thummel, 2003, Baker et al., 1996, Fleming et al., 1997, Huang and Fischer-Vize, 1996, Bello et al., 2006, Mikeladze-Dvali et al., 2005, Palanker et al., 2006, Kim et al., 2006, Bruckner et al., 2004, Lawrence et al., 2004, Bello et al., 2007)
Molecular data
Construct: Ecol\lacZ coding region tagged with T:SV40\nls2 is expressed under the control of Scer\UAS regulatory sequences.
(Bloomington Drosophila Stock Center, 1998.9.14)
Phenotypic class
Phenotype manifest in
Other information
hidew+mC
Reference(s) (Bloomington Drosophila Stock Center, 1998.9.14)
Molecular data
Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
 
4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site.
(Pirrotta, 1988)
Minigene of the white cDNA.
(Chung et al., 1993)
Deletion of w+mC 5' flanking sequences to position -305.
(Qian and Pirrotta, 1995)
Phenotypic class
courtship defective | male
(Lloyd et al., 2002, Hing and Carlson, 1996)
visible | dominant
(True et al., 1996)
visible | recessive
(Roseman et al., 1993)
eye color defective
(Soldatov et al., 1999, Hagstrom et al., 1996)
eye color defective, with Scer\GAL4Act5C.PI, wSym.Scer\UAS, wa.Sym.Scer\UAS
(Giordano et al., 2002)
Phenotype manifest in
pigment cell
(Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995)
pigment cell, with Scer\GAL4Act5C.PI, wSym.Scer\UAS, wa.Sym.Scer\UAS
(Giordano et al., 2002)
Other information
hide Expression Data
Reporter Expression
Stage
Tissue/Position
Reference
Additional Information
Statement
Reference
Scer\GAL4-targeted reporter: expression of Ecol\lacZ reflects action of enhancer/promoter elements driving GAL4 in the same cell.Product localized to nucleus: the β-galactosidase protein produced carries a nuclear localization signal.
(Huang and Fischer-Vize, 1996)
Scer\GAL4-targeted reporter; β-galactosidase distribution reflects action of enhancer/promoter elements driving GAL4 in the same cell.Product localized to nucleus: carries T:SV40\nls2 nuclear localization signal.
(Huang and Fischer-Vize, 1996)
Assay mode
Marker for
Reflects expression of
hide Progenitors & Descendants
progenitor(s)
(Bloomington Drosophila Stock Center, 1998.9.14)
descendant(s)
hide Comments
report comment: Produces β-galactosidase containing the SV40\nls2 nuclear localization signal.
(Bloomington Drosophila Stock Center, 1998.9.14)
mapping_comment: β-gal localized to the nucleus.
(Bloomington Drosophila Stock Center, 1998.9.14)
construct_comment: Progenitors are those of the plasmid pP{UAS-lacZ.NZ}.Yash Hiromi and S. West, unpublished.
(Bloomington Drosophila Stock Center, 1998.9.14)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
NLS-β-Gal
(Shi and Skeath, 2004)
P{@w+mC@ @Ecol\lacZScer\UAS.T:SV40\nls2@=@UAS-lacZ.NZ@}
(FlyBase, 1996-)
P{@w+mC@ @lacZT:nls2@=@UAS-lacZ.NZ@}
(FlyBase, 1996-)
P{UAS-lacZ.NZ}
(FlyBase, 1996-, Kim et al., 2006)
P{UAS-NZ}
(Bloomington Drosophila Stock Center, 1998.9.14)
UAS.lacZ
(Lawrence et al., 2004)
UAS.lacZJ312
(Lawrence et al., 2002)
UAS-lacZ
(Sato et al., 1999)
UAS-lacZ:nls
(Mikeladze-Dvali et al., 2005)
UAS-lacZ.NZ
(Ward et al., 2002)
UAS-LacZ.NZ
(Hatini and DiNardo, 2001)
UAS-lacZnls
(Dominguez et al., 2004)
UAS-lacZnls
(Sen et al., 2004, Bruckner et al., 2004, Bruckner et al., 2004)
UAS-nlacZ
(Astle et al., 2003, Kozlova and Thummel, 2003, Baker et al., 2003, Bello et al., 2003, Kozlova and Thummel, 2002, Badenhorst, 2001, Palanker et al., 2006, Baker et al., 2007)
UAS-nls-lacZ
(Monier et al., 2005, Rival et al., 2004, Rival et al., 2004)
UAS-nlsLacZ
(Cenci and Gould, 2005, Wang et al., 2006)
UAS-NLS-lacZ
(Yamada et al., 2003)
UAS-nlslacZ
(Brodu et al., 2002, Bello et al., 2007)
UAS-nlslacZJ312
(Bello et al., 2006)
UASnuclacZ
(Rangarajan et al., 1999)
UAS-nuclear lacZ
(Jhaveri et al., 2000)
UAS-NZ
(Lee and Park, 2004, Suzuki and Saigo, 2000)
Secondary FlyBase IDs
    hide References ( 52 )
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    hide Recent research papers ( 6 )
    Baker et al., 2007, Genes Dev. 21(4): 450--464
    Functional interactions between the Moses corepressor and DHR78 nuclear receptor regulate growth in Drosophila. [FBrf0193212]
    Bello et al., 2007, Development 134(6): 1091--1099
    Polycomb group genes are required for neural stem cell survival in postembryonic neurogenesis of Drosophila. [FBrf0202325]
    Bello et al., 2006, Development 133(14): 2639--2648
    The brain tumor gene negatively regulates neural progenitor cell proliferation in the larval central brain of Drosophila. [FBrf0194745]
    Kim et al., 2006, Gene Expression Patterns 6(5): 504--518
    Characterization of big bang, a novel gene encoding for PDZ domain-containing proteins that are dynamically expressed throughout Drosophila development. [FBrf0193930]
    Palanker et al., 2006, Development 133(18): 3549--3562
    Dynamic regulation of Drosophila nuclear receptor activity in vivo. [FBrf0193912]
    Wang et al., 2006, Genes Dev. 20(24): 3453--3463
    Aurora-A acts as a tumor suppressor and regulates self-renewal of Drosophila neuroblasts. [FBrf0194325]
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    All reviews listed in FlyBase were published before 2006
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