A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Recombinant construct P{GAL4-Act5C(-FRT).P}

General Information
Symbol P{GAL4-Act5C(-FRT).P} FlyBase ID FBtp0001641
Feature type transgenic_transposon
Size Expression data GAL4 reporter/driver
Associated insertions 0 available
Molecular map
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Description
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FB2013_03
FB2013_02
Controlled Vocabulary Terms
All updates Click here to see a list of all updates to this record from FB2010_08 and on.
hide Description & Uses
Species synthetic construct
Location-dependent
role
Description
CV term
Qualifiers & info
Reference
transposon
Uses
CV term
Qualifiers & info
Reference
analytical tool
mosaic analysis (binary system)
binary system (regulatory)
Cloning Sites
Location
Restriction sites
Reference
hide Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ /
EMBL /
GenBank
DNA sequence
Extent
 
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Total Size
Left end
Right end
Segments
Number
Orientation
Symbol
Reference
hide Features
CV term
Qualifiers & info
Reference
binary
chimeric
general
reporter
hide Component Alleles
Allele Scer\GAL4Act5C.PP
Reference(s) (Reynolds-Kenneally and Mlodzik, 2005, Peri et al., 2002, Wang et al., 2005, Chern and Choi, 2002, Besse et al., 2002, Burke et al., 1999, Sapir et al., 2005, Dong et al., 2000, Collins and Cohen, 2005, Verdu et al., 1999, Perrin et al., 2003, Raisin et al., 2003, McClure and Schubiger, 2005, Rangarajan et al., 1999, Juhasz et al., 2003, Stocker et al., 2003, Saucedo et al., 2003, Hasson et al., 2001, Tulina and Matunis, 2001, Weihe et al., 2001, Britton et al., 2002, Kopp and Duncan, 2002, Amanai and Jiang, 2001, Martin-Castellanos and Edgar, 2002, Rogers et al., 2005, Xin et al., 2002, Chamoun et al., 2001, Rangarajan et al., 2001, Frank et al., 2002, Fabrizio et al., 2003, Szuplewski et al., 2003, Chu et al., 2002, Milan et al., 2001, Jia et al., 2005, Milan and Cohen, 2003, Besse and Pret, 2003, Emerald et al., 2003, Xi et al., 2003, Culi and Mann, 2003, Galant et al., 2002, Parker et al., 2002, Giraldez et al., 2002, Wu and Cohen, 2002, Milan et al., 2002, Jia et al., 2002, Hennig and Neufeld, 2002, Somogyi and Rorth, 2004, Huh et al., 2004, Gaziova et al., 2004, Tseng and Hariharan, 2002, Jia et al., 2003, Hipfner et al., 2002, Radimerski et al., 2002, Bessa et al., 2002, Stevaux et al., 2002, Deng et al., 2003, Bai and Montell, 2002, Estella et al., 2003, Wittwer et al., 2005, Pantalacci et al., 2003, Ikeya et al., 2002, Pierce et al., 2004, Torroja et al., 2004, Ahmad and Baker, 2002, Rosin et al., 2004, Jhaveri et al., 2004, Yoshida et al., 2004, Denef et al., 2000, Fortier and Belote, 2000, Frei and Edgar, 2004, Datar et al., 2000, Prober and Edgar, 2000, Patel et al., 2009, Sisson et al., 2006, Dahmann and Basler, 2000, de la Cova et al., 2004, Reis and Edgar, 2004, Zhang et al., 2000, Brogiolo et al., 2001, Wolf and Schuh, 2000, Milan and Cohen, 2000, Entchev et al., 2000, Certel et al., 2000, Dobens et al., 2000, Luo et al., 1999, Jarecki et al., 1999, Willert et al., 1999, Erkner et al., 1999, Weinkove et al., 1999, Chao and Nagoshi, 1999, Kopp et al., 1999, Dominguez, 1999, Wu and Cohen, 1999, Larkin et al., 1999, Frei et al., 2005, Halder et al., 1998, Methot and Basler, 1999, Gong et al., 1999, Ho et al., 2005, Schlichting et al., 2005, Methot and Basler, 2000, Strigini and Cohen, 1997, Rulifson et al., 2000, Wang et al., 2000, Sustar and Schubiger, 2005, Gorfinkiel et al., 1997, Pichaud and Casares, 2000, Porsch et al., 2005, Shcherbata et al., 2004, Bras-Pereira et al., 2006, Zirin and Mann, 2004, Truman et al., 2004, Scott et al., 2004, Bateman and McNeill, 2004, Miron et al., 2001, Zhang and Kalderon, 2001, Halfar et al., 2001, Keisman and Baker, 2001, Britton and Edgar, 1998, Lee and Treisman, 2001, Cadigan et al., 1998, Lee et al., 2001, Tapon et al., 2001, Neumann and Cohen, 1998, Neufeld et al., 1998, Abu-Shaar and Mann, 1998, Dobens et al., 2001, Hazelett et al., 1998, Dong et al., 2001, Sen et al., 2003, Johnston and Sanders, 2003, Pai et al., 1998, Pignoni and Zipursky, 1997, Kozopas et al., 1998, Casares and Mann, 1998, Johnston et al., 1999, Lim and Tomlinson, 2006, Pallavi et al., 2006, Betschinger et al., 2006, Dussillol-Godar et al., 2006, Croker et al., 2006, Nolo et al., 2006, Poulton and Deng, 2006, Mirkovic and Mlodzik, 2006, Dolezelova et al., 2007, Zhang et al., 2006, Vereshchagina and Wilson, 2006, Lawrence et al., 2004, Miura et al., 2006, Tang et al., 2011, Simcox et al., 2008, Kockel et al., 2010, Wang and Jiang, 2004, Callejo et al., 2006, Bejarano and Milán, 2009, Schwinkendorf and Gallant, 2009, Scott et al., 2007, Scott et al., 2007, Arsham and Neufeld, 2009, Schlichting and Dahmann, 2008, Ting et al., 2007, Wang and Kalderon, 2009, Grewal et al., 2007, Juhász et al., 2008, Narbonne-Reveau et al., 2008, Usha and Shashidhara, 2010, Taniue et al., 2010, Protzer et al., 2008, Neto-Silva et al., 2010, Mitchell et al., 2010, Kawamori and Yamaguchi, 2011, Cranna et al., 2011, Tong et al., 2011, Vaccari et al., 2010, Pircs et al., 2012, Yatsenko et al., 2009, Miller et al., 2008)
Molecular data
The Scer\FRT cassette has been excised, allowing expression of Scer\GAL4 from the Act5C promoter.
Scer\FRT cassette containing Rnor\CD2A902 is excised, juxtaposing Act5C promoter with Scer\GAL4 coding sequences.
Phenotypic class
Phenotype manifest in
autophagic vacuole & larval fat body | somatic clone | cell autonomous, with Sec61αNIG.9539R
autophagic vacuole & larval fat body | somatic clone | cell autonomous, with Sec61αScer\UAS.cKa.T:Zzzz\FLAG
autophagic vacuole & larval fat body | somatic clone | cell autonomous, with Sec61βGD3787
autophagic vacuole & larval fat body | somatic clone | cell autonomous, with Sec61γGD4660
autophagic vacuole & larval fat body | somatic clone | cell autonomous, with Srp14GD13498
autophagic vacuole & larval fat body | somatic clone | cell autonomous, with Srp54kGD1542
autophagic vacuole & larval fat body | somatic clone | cell autonomous, with Srp68GD11522
autophagic vacuole & larval fat body | somatic clone | cell autonomous, with SrpRβGD2371
autophagic vacuole & larval fat body | somatic clone | cell autonomous, with Xbp1Scer\UAS.RB
cell surface & nurse cell | somatic clone, with Pi3K92EScer\UAS.T:Hsap\MYC
eye disc & photoreceptor cell, with dppScer\UAS.cFa
eye disc & photoreceptor cell, with Ras85DV12.Scer\UAS
eye disc & subretinal glial cell, with dppScer\UAS.cFa
follicle cell & nucleus | somatic clone, with CycEScer\UAS.cLa
follicle cell & nucleus | somatic clone, with dapScer\UAS.cdNa
follicle cell & nucleus | somatic clone, with dmScer\UAS.cZa
follicle cell & nucleus | somatic clone, with rapScer\UAS.cSa
follicle cell & nucleus | somatic clone, with rprScer\UAS.cCa
follicle cell & nucleus | somatic clone, with WScer\UAS.cCa
glial cell & eye | ectopic, with dppScer\UAS.cSa
glial cell & eye disc | somatic clone, with ciZn:C.Scer\UAS
glial cell & eye disc | somatic clone | cell non-autonomous, with hhScer\UAS.cAa
imaginal disc & cell, with Pi3K21BScer\UAS.T:Ivir\HA1
imaginal disc & cell, with Pi3K92EScer\UAS.T:Hsap\MYC
leg & cuticle | dorsal | somatic clone, with tshScer\UAS.cGa
leg & cuticle | somatic clone, with tshScer\UAS.cGa
leg & cuticle | ventral | somatic clone, with tshScer\UAS.cGa
lysosome & larval fat body | somatic clone | cell autonomous, with Aats-leuNIG.7479R
lysosome & larval fat body | somatic clone | cell autonomous, with Aats-metNIG.8684R
lysosome & larval fat body | somatic clone | cell autonomous, with Aats-pheNIG.13348R
lysosome & larval fat body | somatic clone | cell autonomous, with Aats-proNIG.12186R
lysosome & larval fat body | somatic clone | cell autonomous, with Aats-tyrdsRNA.Scer\UAS.NIG
lysosome & larval fat body | somatic clone | cell autonomous, with RFeSPNIG.7361R
lysosome & larval fat body | somatic clone | cell autonomous, with Sec61αNIG.9539R
lysosome & larval fat body | somatic clone | cell autonomous, with Sec61αScer\UAS.cKa.T:Zzzz\FLAG
lysosome & larval fat body | somatic clone | cell autonomous, with Sec61βGD3787
lysosome & larval fat body | somatic clone | cell autonomous, with Sec61γGD4660
lysosome & larval fat body | somatic clone | cell autonomous, with Srp14GD13498
lysosome & larval fat body | somatic clone | cell autonomous, with Srp54kGD1542
lysosome & larval fat body | somatic clone | cell autonomous, with Srp68GD11522
lysosome & larval fat body | somatic clone | cell autonomous, with SrpRβGD2371
lysosome & larval fat body | somatic clone | cell autonomous, with Xbp1Scer\UAS.RB
nurse cell & cytoskeleton | somatic clone, with rprScer\UAS.cCa
nurse cell & nucleus | somatic clone, with rprScer\UAS.cCa
photoreceptor cell & axon, with NetAScer\UAS.cHa
photoreceptor cell & axon, with NetBScer\UAS.cHa
Other information
hide Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Information
Statement
Reference
Designed for use in mosaic analyses; used to drive gene of interest in Scer\FLP1-generated clones.
Transiently generated during mosaic analyses; ubiquitous expression throughout region of the generated clone, but not in rest of body (cell autonomous).
Marker for
Reflects expression of
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progenitor(s)
descendant(s)
hide Comments
construct_comment: Contains a single FRT site.
construct_comment: Exists in clones, not reported as being germ-line transmitted.
report comment: Selectable marker not specified.
hide Stocks Listed in FlyBase ( 0 )
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Other Crossreferences
Linkouts
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
Act5CGal4
Act>cd2>GAL4
Act>Gal4
Act > Gal4
Act > y+ > Gal4
actin5c>GAL4
actin5C-Gal4
Actin5Cp-"flip-out"-GAL4
Actin<CD2<Gal4
ActinGAL4
actinGAL4
flip-out GAL4
P{GAL4-Act5C.P}
P{GAL4-Act5C(-FRT).P}
Secondary FlyBase IDs
hide References ( 177 )
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hide Recent research papers ( 5 )
Pircs et al., 2012, PLoS ONE 7(8): e44214
Advantages and Limitations of Different p62-Based Assays for Estimating Autophagic Activity in Drosophila. [FBrf0219364]
Cranna et al., 2011, Fly 5(2): 129--133
Hfp, the Drosophila homolog of the mammalian c-myc transcriptional-repressor and tumor suppressor FIR, inhibits dmyc transcription and cell growth. [FBrf0214410]
Kawamori and Yamaguchi, 2011, Cell Struct. Funct. 36(1): 103--119
DREF is Critical for Drosophila Bristle Development by Regulating Endoreplication in Shaft Cells. [FBrf0213809]
Tang et al., 2011, EMBO J. 30(4): 636--651
Atg1-mediated myosin II activation regulates autophagosome formation during starvation-induced autophagy. [FBrf0213047]
Tong et al., 2011, Neuron 71(3): 447--459
Rich Regulates Target Specificity of Photoreceptor Cells and N-Cadherin Trafficking in the Drosophila Visual System via Rab6. [FBrf0214681]