Recombinant construct P{GAL4-Act5C(-FRT).P}
| General Information | |||
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| Symbol | P{GAL4-Act5C(-FRT).P} | FlyBase ID | FBtp0001641 |
| Feature type | transgenic_transposon | ||
| Size | Expression data | GAL4 reporter/driver | |
| Associated insertions | 0 available | ||
| Molecular map | |||
Recent Updates
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| Description |
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| FB2013_03 | |||
| FB2013_02 |
Controlled Vocabulary Terms
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| All updates | Click here to see a list of all updates to this record from FB2010_08 and on. | ||
Description & Uses
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| Species | synthetic construct | ||
| Location-dependent
role |
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| Description |
CV term
Qualifiers & info
Reference
transposon
P-element
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| Uses |
CV term
Qualifiers & info
Reference
analytical tool
mosaic analysis (binary system)
binary system (regulatory)
Act5C
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| Cloning Sites |
Location
Restriction sites
Reference
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Sequence Data
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| Sequence (FB) | |||
| Associated Sequence Data | |||
| DDBJ
/
EMBL / GenBank |
DNA sequence
Extent
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Segments & Size
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| Total Size | |||
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| Right end | |||
| Segments |
Number
Orientation
Symbol
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| Reference | |||
Features
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CV term
Qualifiers & info
Reference
binary
component
chimeric
gene
general
promoter
reporter
gene
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Component Alleles
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| Allele | Scer\GAL4Act5C.PP | ||
| Reference(s) | (Reynolds-Kenneally and Mlodzik, 2005, Peri et al., 2002, Wang et al., 2005, Chern and Choi, 2002, Besse et al., 2002, Burke et al., 1999, Sapir et al., 2005, Dong et al., 2000, Collins and Cohen, 2005, Verdu et al., 1999, Perrin et al., 2003, Raisin et al., 2003, McClure and Schubiger, 2005, Rangarajan et al., 1999, Juhasz et al., 2003, Stocker et al., 2003, Saucedo et al., 2003, Hasson et al., 2001, Tulina and Matunis, 2001, Weihe et al., 2001, Britton et al., 2002, Kopp and Duncan, 2002, Amanai and Jiang, 2001, Martin-Castellanos and Edgar, 2002, Rogers et al., 2005, Xin et al., 2002, Chamoun et al., 2001, Rangarajan et al., 2001, Frank et al., 2002, Fabrizio et al., 2003, Szuplewski et al., 2003, Chu et al., 2002, Milan et al., 2001, Jia et al., 2005, Milan and Cohen, 2003, Besse and Pret, 2003, Emerald et al., 2003, Xi et al., 2003, Culi and Mann, 2003, Galant et al., 2002, Parker et al., 2002, Giraldez et al., 2002, Wu and Cohen, 2002, Milan et al., 2002, Jia et al., 2002, Hennig and Neufeld, 2002, Somogyi and Rorth, 2004, Huh et al., 2004, Gaziova et al., 2004, Tseng and Hariharan, 2002, Jia et al., 2003, Hipfner et al., 2002, Radimerski et al., 2002, Bessa et al., 2002, Stevaux et al., 2002, Deng et al., 2003, Bai and Montell, 2002, Estella et al., 2003, Wittwer et al., 2005, Pantalacci et al., 2003, Ikeya et al., 2002, Pierce et al., 2004, Torroja et al., 2004, Ahmad and Baker, 2002, Rosin et al., 2004, Jhaveri et al., 2004, Yoshida et al., 2004, Denef et al., 2000, Fortier and Belote, 2000, Frei and Edgar, 2004, Datar et al., 2000, Prober and Edgar, 2000, Patel et al., 2009, Sisson et al., 2006, Dahmann and Basler, 2000, de la Cova et al., 2004, Reis and Edgar, 2004, Zhang et al., 2000, Brogiolo et al., 2001, Wolf and Schuh, 2000, Milan and Cohen, 2000, Entchev et al., 2000, Certel et al., 2000, Dobens et al., 2000, Luo et al., 1999, Jarecki et al., 1999, Willert et al., 1999, Erkner et al., 1999, Weinkove et al., 1999, Chao and Nagoshi, 1999, Kopp et al., 1999, Dominguez, 1999, Wu and Cohen, 1999, Larkin et al., 1999, Frei et al., 2005, Halder et al., 1998, Methot and Basler, 1999, Gong et al., 1999, Ho et al., 2005, Schlichting et al., 2005, Methot and Basler, 2000, Strigini and Cohen, 1997, Rulifson et al., 2000, Wang et al., 2000, Sustar and Schubiger, 2005, Gorfinkiel et al., 1997, Pichaud and Casares, 2000, Porsch et al., 2005, Shcherbata et al., 2004, Bras-Pereira et al., 2006, Zirin and Mann, 2004, Truman et al., 2004, Scott et al., 2004, Bateman and McNeill, 2004, Miron et al., 2001, Zhang and Kalderon, 2001, Halfar et al., 2001, Keisman and Baker, 2001, Britton and Edgar, 1998, Lee and Treisman, 2001, Cadigan et al., 1998, Lee et al., 2001, Tapon et al., 2001, Neumann and Cohen, 1998, Neufeld et al., 1998, Abu-Shaar and Mann, 1998, Dobens et al., 2001, Hazelett et al., 1998, Dong et al., 2001, Sen et al., 2003, Johnston and Sanders, 2003, Pai et al., 1998, Pignoni and Zipursky, 1997, Kozopas et al., 1998, Casares and Mann, 1998, Johnston et al., 1999, Lim and Tomlinson, 2006, Pallavi et al., 2006, Betschinger et al., 2006, Dussillol-Godar et al., 2006, Croker et al., 2006, Nolo et al., 2006, Poulton and Deng, 2006, Mirkovic and Mlodzik, 2006, Dolezelova et al., 2007, Zhang et al., 2006, Vereshchagina and Wilson, 2006, Lawrence et al., 2004, Miura et al., 2006, Tang et al., 2011, Simcox et al., 2008, Kockel et al., 2010, Wang and Jiang, 2004, Callejo et al., 2006, Bejarano and Milán, 2009, Schwinkendorf and Gallant, 2009, Scott et al., 2007, Scott et al., 2007, Arsham and Neufeld, 2009, Schlichting and Dahmann, 2008, Ting et al., 2007, Wang and Kalderon, 2009, Grewal et al., 2007, Juhász et al., 2008, Narbonne-Reveau et al., 2008, Usha and Shashidhara, 2010, Taniue et al., 2010, Protzer et al., 2008, Neto-Silva et al., 2010, Mitchell et al., 2010, Kawamori and Yamaguchi, 2011, Cranna et al., 2011, Tong et al., 2011, Vaccari et al., 2010, Pircs et al., 2012, Yatsenko et al., 2009, Miller et al., 2008) | ||
| Molecular data |
Scer\FRT cassette containing Rnor\CD2A902 is excised, juxtaposing Act5C promoter with Scer\GAL4 coding sequences.
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| Phenotypic class |
lethal, with biScer\UAS.cPa
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| Phenotype manifest in |
autophagic vacuole & larval fat body | somatic clone | cell autonomous, with Sec61αNIG.9539R
autophagic vacuole & larval fat body | somatic clone | cell autonomous, with Sec61αScer\UAS.cKa.T:Zzzz\FLAG
autophagic vacuole & larval fat body | somatic clone | cell autonomous, with Sec61βGD3787
autophagic vacuole & larval fat body | somatic clone | cell autonomous, with Sec61γGD4660
autophagic vacuole & larval fat body | somatic clone | cell autonomous, with Srp14GD13498
autophagic vacuole & larval fat body | somatic clone | cell autonomous, with Srp54kGD1542
autophagic vacuole & larval fat body | somatic clone | cell autonomous, with Srp68GD11522
autophagic vacuole & larval fat body | somatic clone | cell autonomous, with SrpRβGD2371
autophagic vacuole & larval fat body | somatic clone | cell autonomous, with Xbp1Scer\UAS.RB
cell, with dmScer\UAS.cZa
cell surface & nurse cell | somatic clone, with Pi3K92EScer\UAS.T:Hsap\MYC
eye, with hthScer\UAS.cPa
eye disc & photoreceptor cell, with dppScer\UAS.cFa
eye disc & photoreceptor cell, with Ras85DV12.Scer\UAS
eye disc & subretinal glial cell, with dppScer\UAS.cFa
follicle cell & nucleus | somatic clone, with CycEScer\UAS.cLa
follicle cell & nucleus | somatic clone, with dapScer\UAS.cdNa
follicle cell & nucleus | somatic clone, with dmScer\UAS.cZa
follicle cell & nucleus | somatic clone, with rapScer\UAS.cSa
follicle cell & nucleus | somatic clone, with rprScer\UAS.cCa
follicle cell & nucleus | somatic clone, with WScer\UAS.cCa
glial cell & eye | ectopic, with dppScer\UAS.cSa
glial cell & eye disc | somatic clone, with ciZn:C.Scer\UAS
glial cell & eye disc | somatic clone | cell non-autonomous, with hhScer\UAS.cAa
imaginal disc & cell, with Pi3K21BScer\UAS.T:Ivir\HA1
imaginal disc & cell, with Pi3K21BScer\UAS.Δ.T:Ivir\HA1
imaginal disc & cell, with Pi3K92ED954A.Scer\UAS.T:Hsap\MYC
imaginal disc & cell, with Pi3K92EScer\UAS.T:Hsap\MYC
lamina, with NetAScer\UAS.cHa
lamina, with NetBScer\UAS.cHa
leg & cuticle | dorsal | somatic clone, with tshScer\UAS.cGa
leg & cuticle | somatic clone, with tshScer\UAS.cGa
leg & cuticle | ventral | somatic clone, with tshScer\UAS.cGa
lysosome & larval fat body | somatic clone | cell autonomous, with Aats-leuNIG.7479R
lysosome & larval fat body | somatic clone | cell autonomous, with Aats-metNIG.8684R
lysosome & larval fat body | somatic clone | cell autonomous, with Aats-pheNIG.13348R
lysosome & larval fat body | somatic clone | cell autonomous, with Aats-proNIG.12186R
lysosome & larval fat body | somatic clone | cell autonomous, with Aats-tyrdsRNA.Scer\UAS.NIG
lysosome & larval fat body | somatic clone | cell autonomous, with RFeSPNIG.7361R
lysosome & larval fat body | somatic clone | cell autonomous, with Sec61αNIG.9539R
lysosome & larval fat body | somatic clone | cell autonomous, with Sec61αScer\UAS.cKa.T:Zzzz\FLAG
lysosome & larval fat body | somatic clone | cell autonomous, with Sec61βGD3787
lysosome & larval fat body | somatic clone | cell autonomous, with Sec61γGD4660
lysosome & larval fat body | somatic clone | cell autonomous, with Srp14GD13498
lysosome & larval fat body | somatic clone | cell autonomous, with Srp54kGD1542
lysosome & larval fat body | somatic clone | cell autonomous, with Srp68GD11522
lysosome & larval fat body | somatic clone | cell autonomous, with SrpRβGD2371
lysosome & larval fat body | somatic clone | cell autonomous, with Xbp1Scer\UAS.RB
nurse cell & cytoskeleton | somatic clone, with rprScer\UAS.cCa
nurse cell & nucleus | somatic clone, with rprScer\UAS.cCa
photoreceptor cell & axon, with NetAScer\UAS.cHa
photoreceptor cell & axon, with NetBScer\UAS.cHa
tibia, with hthScer\UAS.cPa
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| Other information | |||
Expression Data
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| Reporter Expression | |||
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distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
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| Additional Information | |||
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Statement
Reference
Designed for use in mosaic analyses; used to drive gene of interest in Scer\FLP1-generated clones.
Transiently generated during mosaic analyses; ubiquitous expression throughout region of the generated clone, but not in rest
of body (cell autonomous).
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| Marker for | |||
| Reflects expression of | |||
Progenitors & Descendants
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Comments
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construct_comment: Contains a single FRT site.
construct_comment: Exists in clones, not reported as being germ-line transmitted.
report comment: Selectable marker not specified.
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Stocks Listed in FlyBase
( 0 )
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External Crossreferences & Linkouts
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| Other Crossreferences | |||
| Linkouts | |||
Synonyms & Secondary IDs
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| Reported As | |||
| Symbol Synonym |
Act5C>GAL4
Act5CGal4
act5C-GAL4
Act5C-Gal4
Act5C-GAL4
act.Gal4
Act>cd2>GAL4
Act>Gal4
Act > Gal4
Act > y+ > Gal4
Act-GAL4
act-Gal4
act-gal4
actin5c>GAL4
actin5C-Gal4
Actin5Cp-"flip-out"-GAL4
Actin<CD2<Gal4
ActinGAL4
actinGAL4
actin-Gal4
actinGal4
actin-GAL4
flip GAL4
flip-out GAL4
P{GAL4-Act5C.P}
P{GAL4-Act5C(-FRT).P}
unnamed
(Scott et al., 2007, Pallavi et al., 2006, Wittwer et al., 2005, Vaccari et al., 2010, Miura et al., 2006, Simcox et al., 2008, Callejo et al., 2006, Bejarano and Milán, 2009, Scott et al., 2007, Schwinkendorf and Gallant, 2009, Arsham and Neufeld, 2009, Wang and Kalderon, 2009, Juhász et al., 2008, Narbonne-Reveau et al., 2008, Taniue et al., 2010)
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| Secondary FlyBase IDs | |||
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References
( 177 )
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| Generate a list of | |||
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Recent research papers ( 5 )
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Recent Updates
External Crossreferences & Linkouts