Recombinant construct P{UAS-rpr.Z}

General Information
Symbol	P{UAS-rpr.Z}	FlyBase ID	FBtp0001647
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	2 available
Molecular map
Recent Updates
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FB2013_03
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Zhou et al., 1997)
Uses	CV term Qualifiers & info Reference binary system (targeted) rpr (Zhou et al., 1997) characterization rpr (Zhou et al., 1997)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference binary component (Zhou et al., 1997) chimeric gene (Zhou et al., 1997) selectable marker (Zhou et al., 1997, McNabb et al., 1997)
Component Alleles
Allele	rpr^Scer\UAS.cZa
Reference(s)	(Clark et al., 2004, Keller et al., 2002, Rodriguez Moncalvo and Campos, 2005, Renn et al., 1999, Park et al., 2003, Robertson et al., 2003, Malpel et al., 2002, Mendes et al., 2009, Geisbrecht and Montell, 2004, Dewey et al., 2004, Wing et al., 2002, Lee and Park, 2004, Ryoo et al., 2002, Mergliano and Minden, 2003, Tanoue et al., 2004, Myat and Andrew, 2000, Baker et al., 1999, Wing et al., 1998, McNabb et al., 1997, Wing et al., 2001, Chen et al., 1998, Zhou et al., 1997, Kim et al., 2006, Wells et al., 2006, Lee et al., 2006, Ryoo et al., 2004, Perez-Garijo et al., 2005, Brun et al., 2006, Pereanu et al., 2007, Kanuka et al., 2005, Palgi et al., 2009, Picot et al., 2009, Mitri et al., 2009, Kuraishi et al., 2009, Morris et al., 2006, Koto et al., 2009, Hassan et al., 2005, Choi et al., 2008, Yoshii et al., 2005, Pereanu et al., 2007, Terhzaz et al., 2007, Schroeder et al., 2003, Fishilevich et al., 2010, Blanco et al., 2010, Wang et al., 2008, Fox and Spradling, 2009, Sinenko et al., 2011, Bahn et al., 2009, Ben-Shahar et al., 2010, McNabb and Truman, 2008)
Molecular data	Construct: A 0.9kb EcoRI fragment containing rpr cDNA is expressed under the control of Scer\UAS regulatory sequences. (McNabb et al., 1997) rpr cDNA cloned into pUAST. (Zhou et al., 1997)
Phenotypic class	behavior defective \| embryonic stage, with Scer\GAL4^gcm-rA87.C (Pereanu et al., 2007) behavior defective \| larval stage, with Scer\GAL4^nrv2.PS (Pereanu et al., 2007) cell death defective, with Scer\GAL4^Eh.2.4 (McNabb et al., 1997) courtship behavior defective \| male, with Scer\GAL4^IFa.PT (Terhzaz et al., 2007) courtship behavior defective \| male, with Scer\GAL4^npf.1 (Lee et al., 2006) eclosion rhythm defective, with Scer\GAL4^Ccap.PP (Park et al., 2003) eclosion rhythm defective, with Scer\GAL4^Eh.2.4 (McNabb et al., 1997, McNabb and Truman, 2008) feeding behavior defective, with Scer\GAL4^Gr66a.PD (Mitri et al., 2009) fertile, with Scer\GAL4^Akh.PL (Lee and Park, 2004) increased cell death, with Scer\GAL4^Ccap.PP (Clark et al., 2004) increased cell death, with Scer\GAL4^Eh.2.4 (Clark et al., 2004) increased cell death, with Scer\GAL4^en-e16E (Ryoo et al., 2002) increased cell death, with Scer\GAL4^GMR.PF (Wing et al., 1998, Wing et al., 2001) increased cell death, with Scer\GAL4^ninaE.PT (Mendes et al., 2009) increased cell death, with Scer\GAL4^P2.4.Pdf (Rodriguez Moncalvo and Campos, 2005) increased cell death, with Scer\GAL4^P2.4.Pdf, Scer\GAL4^Rh5.PT (Rodriguez Moncalvo and Campos, 2005) increased cell death, with Scer\GAL4^salm.PU (Blanco et al., 2010) increased cell death, with Scer\GAL4^slbo.2.6 (Geisbrecht and Montell, 2004) lethal (Morris et al., 2006) lethal, with Scer\GAL4^30A (Morris et al., 2006) lethal, with Scer\GAL4³¹ (Morris et al., 2006) lethal, with Scer\GAL4^32B (Morris et al., 2006) lethal, with Scer\GAL4^34B (Morris et al., 2006) lethal, with Scer\GAL4^71B (Morris et al., 2006) lethal, with Scer\GAL4^179Y (Morris et al., 2006) lethal, with Scer\GAL4³⁷³⁵ (Morris et al., 2006) lethal, with Scer\GAL4⁴⁹³⁵ (Morris et al., 2006) lethal, with Scer\GAL4^Act5C.PI (Morris et al., 2006) lethal, with Scer\GAL4^ap-md544 (Morris et al., 2006) lethal, with Scer\GAL4^arm.PS (Morris et al., 2006) lethal, with Scer\GAL4^c323a (Morris et al., 2006) lethal, with Scer\GAL4^Cy6 (Morris et al., 2006) lethal, with Scer\GAL4^da.G32 (Morris et al., 2006) lethal, with Scer\GAL4^Dll-md23 (Morris et al., 2006) lethal, with Scer\GAL4^dpp.blk1 (Morris et al., 2006) lethal, with Scer\GAL4^en-e16E (Morris et al., 2006) lethal, with Scer\GAL4^ey.PH (Morris et al., 2006) lethal, with Scer\GAL4^GMR.PF (Wing et al., 1998, Wing et al., 2001, Morris et al., 2006) lethal, with Scer\GAL4^h-H10 (Morris et al., 2006) lethal, with Scer\GAL4^how-24B (Morris et al., 2006) lethal, with Scer\GAL4^hs.PB (Morris et al., 2006) lethal, with Scer\GAL4^nrv2.PS (Pereanu et al., 2007) lethal, with Scer\GAL4^ple.PF (Schroeder et al., 2003) lethal, with Scer\GAL4^prd.RG1 (Morris et al., 2006) lethal, with Scer\GAL4^sca-T3 (Morris et al., 2006) lethal, with Scer\GAL4^sev.EP (Morris et al., 2006) lethal, with Scer\GAL4^T80 (Morris et al., 2006) lethal, with Scer\GAL4^tsh-md621 (Morris et al., 2006) lethal, with Scer\GAL4^twi.2PE (Morris et al., 2006) lethal, with Scer\GAL4^twi.PG (Morris et al., 2006) lethal, with Scer\GAL4^wg.PM (Morris et al., 2006) lethal, with Scer\GAL4^αTub84B.PL (Morris et al., 2006) lethal \| embryonic stage, with Scer\GAL4^en-e16E (Ryoo et al., 2002) lethal \| pupal stage, with Scer\GAL4^Ccap.PP (Park et al., 2003) lethal \| pupal stage, with Scer\GAL4^GMR.PF (Keller et al., 2002) locomotor rhythm defective, with Scer\GAL4^P2.4.Pdf (Renn et al., 1999) locomotor rhythm defective \| male, with Scer\GAL4^npf.1 (Lee et al., 2006) neuroanatomy defective \| late embryonic stage, with Scer\GAL4^gcm-rA87.C (Pereanu et al., 2007) partially lethal - majority die, with Scer\GAL4^elav-C155 (Morris et al., 2006) partially lethal - majority die, with Scer\GAL4^GMR.PU (Kanuka et al., 2005) partially lethal - majority die \| larval stage, with Scer\GAL4^Eh.2.4 (McNabb et al., 1997) viable, with Scer\GAL4^Akh.PL (Lee and Park, 2004) viable, with Scer\GAL4^rdgC.PK (Keller et al., 2002) viable, with Scer\GAL4^Rh3.PP (Kanuka et al., 2005) visible, with Scer\GAL4^Act88F.PD (Morris et al., 2006) visible, with Scer\GAL4^GMR.PF (Wing et al., 2001) visible, with Scer\GAL4^nos.PG (Morris et al., 2006)
Phenotype manifest in	abdominal dorsal/lateral multidendritic neuron, with Scer\GAL4^Ccap.PP (Kim et al., 2006) adult cuticle, with Scer\GAL4^Eh.2.4 (McNabb et al., 1997) adult head, with Scer\GAL4^Ccap.PP (Park et al., 2003) border follicle cell, with Scer\GAL4^slbo.2.6 (Geisbrecht and Montell, 2004) centripetally migrating follicle cell, with Scer\GAL4^slbo.2.6 (Geisbrecht and Montell, 2004) EH neurosecretory neuron, with Scer\GAL4^Eh.2.4 (Kim et al., 2006, Clark et al., 2004) embryonic/larval brain \| late embryonic stage, with Scer\GAL4^gcm-rA87.C (Pereanu et al., 2007) embryonic/larval mouth, with Scer\GAL4^Ccap.PP, Scer\GAL4^Eh.2.4 (Clark et al., 2004) embryonic/larval mouth, with Scer\GAL4^Eh.2.4 (Clark et al., 2004) embryonic/larval trachea \| dorsal, with Scer\GAL4^Eh.2.4 (McNabb et al., 1997) eye, with Scer\GAL4^GMR.PF (Wing et al., 2001, Koto et al., 2009) eye, with Scer\GAL4^GMR.PU (Kanuka et al., 2005) follicle cell, with Scer\GAL4^slbo.2.6 (Geisbrecht and Montell, 2004) glial cell \| larval stage, with Scer\GAL4^nrv2.PS (Pereanu et al., 2007) leg, with Scer\GAL4^Ccap.PP (Park et al., 2003) LNv neuron, with Scer\GAL4^P2.4.Pdf (Rodriguez Moncalvo and Campos, 2005) macrochaeta, with Scer\GAL4^nos.PG (Morris et al., 2006) macrochaeta, with Scer\GAL4^sca-109-68 (Koto et al., 2009) midline glial cell, with Scer\GAL4^Gp150-52A (Wing et al., 1998) neuron \| larval stage, with Scer\GAL4^Ccap.PP (Park et al., 2003, Clark et al., 2004) neuron \| larval stage, with Scer\GAL4^Eh.2.4 (McNabb et al., 1997) neuropil glial sheath \| late embryonic stage, with Scer\GAL4^gcm-rA87.C (Pereanu et al., 2007) outer photoreceptor cell, with Scer\GAL4^ninaE.PT (Mendes et al., 2009) Pdf neuron, with Scer\GAL4^P2.4.Pdf (Hassan et al., 2005) Pdf neuron, with Scer\GAL4^tim.Scer\UAS (Hassan et al., 2005) photoreceptor cell R5, with Scer\GAL4^Rh5.PT (Rodriguez Moncalvo and Campos, 2005) rhabdomere R7, with Scer\GAL4^Rh3.PP (Kanuka et al., 2005) Tv neuron, with Scer\GAL4^Fmrf.PS (Kim et al., 2006) wing, with Scer\GAL4^Ccap.PP (Park et al., 2003) wing, with Scer\GAL4^Eh.2.4 (McNabb et al., 1997) wing disc, with Scer\GAL4^salm.PU (Blanco et al., 2010) wing vein, with Scer\GAL4^Act88F.PD (Morris et al., 2006)
Other information

Allele	w^+mC
Reference(s)	(Zhou et al., 1997, McNabb et al., 1997)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	pP{UAST} (Zhou et al., 1997)
descendant(s)
Comments
	construct_comment: One of two constructs, not specified which, originating from different rpr cDNA clones.Progenitors are those of the plasmids, pP{UAS-rpr.Z} (two variants). (Zhou et al., 1997)
Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	P[UAS-reaper] (Wing et al., 2001, Wing et al., 1998) P{UAS-rpr.Z} (FlyBase, 1996-) UAS-reaper (Geisbrecht and Montell, 2004, Kim et al., 2006, Choi et al., 2008, Kanuka et al., 2005, McNabb and Truman, 2008) UAS-Reaper (Koto et al., 2009) UAS-rpr (Rodriguez Moncalvo and Campos, 2005, Dewey et al., 2004, Lee and Park, 2004, Tanoue et al., 2004, Mergliano and Minden, 2003, Robertson et al., 2003, Park et al., 2003, Ryoo et al., 2002, Wing et al., 2002, Keller et al., 2002, Malpel et al., 2002, Rulifson et al., 2002, Rulifson et al., 2002, Myat and Andrew, 2000, Renn et al., 1999, Baker et al., 1999, Lee et al., 2006, Ryoo et al., 2004, Blanco et al., 2010, Clark et al., 2004, Pereanu et al., 2007, Picot et al., 2009, Mitri et al., 2009, Kuraishi et al., 2009, Morris et al., 2006, Hassan et al., 2005, Yoshii et al., 2005, Pereanu et al., 2007, Terhzaz et al., 2007, Schroeder et al., 2003, Fishilevich et al., 2010, Wang et al., 2008, Fox and Spradling, 2009, Brun et al., 2006, Ben-Shahar et al., 2010, McNabb and Truman, 2008, Bahn et al., 2009) UAS-Rpr (Wells et al., 2006) uas-rpr (Mendes et al., 2009) unnamed (Sinenko et al., 2011)
Secondary FlyBase IDs

References ( 52 )

Generate a list of	All references relating to this transgenic transposon ( 52 )

List References by type	Research paper ( 50 ) Supplementary material ( 2 )
Recent research papers (0)
	All research papers listed in FlyBase were published before 2011 Show research papers ...