Recombinant construct P{sE-raf^tor4021}

General Information
Symbol	P{sE-raf^tor4021}	FlyBase ID	FBtp0001680
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	0 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Dickson et al., 1992)
Uses	CV term Qualifiers & info Reference characterization phl (Dickson et al., 1992) phl \| fusion construct (Dickson et al., 1992) tor \| fusion construct (Dickson et al., 1992)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference chimeric gene (Dickson et al., 1992) chimeric protein (Dickson et al., 1992) chimeric protein (Dickson et al., 1992) chimeric protein (Dickson et al., 1992) inducible promoter (Dickson et al., 1992) selectable marker (Dickson et al., 1992)
Component Alleles
Allele	phl::tor^13D.hs.sev
Reference(s)	(Dickson et al., 1992, Roy et al., 2002, Reichman-Fried et al., 1994, Chang et al., 1995, Bai et al., 2001, Therrien et al., 1999, Casci et al., 1999, Lu and Li, 1999, Vincent et al., 1998, Kauffmann et al., 1995, Lai et al., 1996, Karim et al., 1996, Allard et al., 1996, Wassarman et al., 1996, Chang et al., 1994, Therrien et al., 1995, Chang and Rubin, 1997, Peverali et al., 1996)
Molecular data	A constitutively active form of phl is expressed under the control of a sev enhancer. (Chang and Rubin, 1997) phl intracellular kinase domain replaces the equivalent region from tor^13D, expression of the chimera is driven by an Hsp70 promoter and a single copy of the 1.2kb sev enhancer. (Reichman-Fried et al., 1994) Construct: phl kinase domain fused to the signal sequence, intracellular and transmembrane domains carrying the amino acid replacement characteristic of the tor^13D protein, expressed from the sev enhancer and a heat shock promoter. (Dickson et al., 1992)
Phenotypic class	visible (Lai et al., 1996, Bai et al., 2001, Kauffmann et al., 1995, Lu and Li, 1999, Roy et al., 2002) visible \| dominant (Chang and Rubin, 1997)
Phenotype manifest in	eye (Wassarman et al., 1996, Lai et al., 1996, Bai et al., 2001, Chang and Rubin, 1997, Therrien et al., 1998, Lu and Li, 1999, Roy et al., 2002) photoreceptor cell R7 (Chang et al., 1995, Kauffmann et al., 1995) photoreceptor cell R7 \| supernumerary (Roy et al., 2002)
Other information

Allele	w^+mW.hs
Reference(s)	(Dickson et al., 1992)
Molecular data	A mini-w gene is expressed under the control of an Hsp70 promoter. (Heinrich et al., 1993) w gene expression is under the influence of FLIP recombinase. (Golic and Lindquist, 1989) Expression of a fragment of w genomic DNA with a 2.2kb deletion of 3' flanking sequences and a reduction in the size of the largest intron to 356bp is driven by a 388bp fragment of an Hsp70 gene. (Klemenz et al., 1987) Construct: A 'mini-white' gene constructed by deletion of the XbaI-HindII fragment from the long 5' intron. Carried by the W6 and W8 series of vectors.
Phenotypic class	suppressor of variegation, with Su(var)205⁵⁰² (Ahmad and Golic, 1996)
Phenotype manifest in	pigment cell (Kurkulos et al., 1994, Golic and Lindquist, 1989)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	pP{W8} (Dickson et al., 1992)
descendant(s)
Comments
	construct_comment: Progenitors are those of the plasmid, pP{sE-raf^tor4021}. (Dickson et al., 1992)
Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	BT98 (Roy et al., 2002) N-Tor⁴⁰²¹RAFc (Roy et al., 2002) P{sE-raf^tor4021} (FlyBase, 1996-) sE-RAF^Tor4021 (Therrien et al., 1999) sev-Raf^tor4021 (Lu and Li, 1999) sev-tor⁴⁰²¹Raf (Bai et al., 2001, Casci et al., 1999)
Secondary FlyBase IDs

References ( 18 )
Research paper	Roy et al., 2002, Genes Dev. 16(4): 427--438 KSR is a scaffold required for activation of the ERK/MAPK module. [FBrf0144882] Bai et al., 2001, Development 128(4): 591--601 The cell adhesion molecule Echinoid defines a new pathway that antagonizes the Drosophila EGF receptor signaling pathway. [FBrf0134572] Casci et al., 1999, Cell 96(5): 655--665 Sprouty, an intracellular inhibitor of Ras signaling. [FBrf0107641] Lu and Li, 1999, Dev. Biol. 208(1): 233--243 Drosophila Src42A is a negative regulator of RTK signaling. [FBrf0107862] Therrien et al., 1999, Proc. Natl. Acad. Sci. U.S.A. 96(23): 13259--13263 Functional analysis of CNK in RAS signaling. [FBrf0112056] Vincent et al., 1998, Mol. Cell 2(4): 515--525 The Drosophila protein Dof is specifically required for FGF signaling. [FBrf0105337] Chang and Rubin, 1997, Genes Dev. 11(9): 1132--1139 14-3-3 epsilon positively regulates Ras-mediated signaling in Drosophila. [FBrf0093395] Allard et al., 1996, Development 122(4): 1137--1146 The SH2-containing tyrosine phosphatase corkscrew is required during signaling by sevenless, Ras1 and Raf. [FBrf0086863] Karim et al., 1996, Genetics 143(1): 315--329 A screen for genes that function downstream of Ras1 during Drosophila eye development. [FBrf0087493] Lai et al., 1996, Proc. Natl. Acad. Sci. U.S.A. 93(10): 5025--5030 Loss of tramtrack gene activity results in ectopic R7 cell formation, even in a sina mutant background. [FBrf0087523] Peverali et al., 1996, EMBO J. 15(15): 3943--3950 Phosphorylation of Drosophila Jun by the MAP kinase rolled regulates photoreceptor differentiation. [FBrf0089778] Wassarman et al., 1996, Genes Dev. 10(3): 272--278 Protein phosphatase 2A positively and negatively regulates Ras1-mediated photoreceptor development in Drosophila. [FBrf0086675] Chang et al., 1995, Cell 80(3): 463--472 phyllopod functions in the fate determination of a subset of photoreceptors in Drosophila. [FBrf0079934] Kauffmann et al., 1995, Proc. Natl. Acad. Sci. U.S.A. 92(24): 10919--10923 Activated Drosophila Ras1 is selectively suppressed by isoprenyl transferase inhibitors. [FBrf0084085] Therrien et al., 1995, Cell 83(6): 879--888 KSR, a novel protein kinase required for RAS signal transduction. [FBrf0086035] Reichman-Fried et al., 1994, Genes Dev. 8(4): 428--439 Elucidation of the role of breathless, a Drosophila FGF receptor homolog, in tracheal cell migration. [FBrf0068648]
Review	Chang et al., 1994, Cold Spring Harbor Symp. Quant. Biol. 59: 147--153 Ras signal transduction pathway in Drosophila eye development. [FBrf0083080]
Letter	Dickson et al., 1992, Nature 360: 600--603 Raf functions downstream of Ras1 in the Sevenless signal transduction pathway. [FBrf0057339]

Recombinant construct P{sE-raftor4021}

Recombinant construct P{sE-raf^tor4021}