Recombinant construct M{MlwB}

General Information
Symbol	M{MlwB}	FlyBase ID	FBtp0001682
Feature type	transgenic_transposon

Size		Expression data	lacZ reporter
Associated insertions	0 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon mariner element (Lidholm et al., 1993)
Uses	CV term Qualifiers & info Reference characterization Dmau\mariner\T (Lidholm et al., 1993) enhancer trap Dmau\mariner (Lidholm et al., 1993)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference chimeric gene (Lidholm et al., 1993) enhancer trap (Lidholm et al., 1993) reporter gene (Lidholm et al., 1993) selectable marker (Lidholm et al., 1993, Lohe and Hartl, 1996, Hartl et al., 1997)
Component Alleles
Allele	Dmau\mariner\T^pA2L
Reference(s)	(Lohe and Hartl, 1996, Lidholm et al., 1993, Lozovsky et al., 2002, Hartl et al., 1997)
Molecular data	Insertion of foreign DNA, including w^+mW.hs, in the SacI site at position 789 of the Mos1 transposable element. (Lohe and Hartl, 1996) Dmau\mariner\T coding region interrupted at position 789 by introduction of w^+mW.hs. (Lohe et al., 1995) Construct: Fragment of Mos1 up to nucleotide 787, including the Dmau\mariner 5' terminal repeat. (Lidholm et al., 1993)
Phenotypic class
Phenotype manifest in
Other information

Allele	Ecol\lacZ^P\T.A92a
Reference(s)	(Lidholm et al., 1993, Hartl et al., 1997)
Molecular data	Construct: In frame translational fusion of P\T to Ecol\lacZ, in the second exon of the P element. The fusion includes the P element promoter. The P-element 5' end is from pUChsneo. (Wilson et al., 1989)
Phenotypic class
Phenotype manifest in
Other information

Allele	w^+mW.hs
Reference(s)	(Lidholm et al., 1993, Lozovsky et al., 2002, Lohe and Hartl, 1996, Hartl et al., 1997)
Molecular data	A mini-w gene is expressed under the control of an Hsp70 promoter. (Heinrich et al., 1993) w gene expression is under the influence of FLIP recombinase. (Golic and Lindquist, 1989) Expression of a fragment of w genomic DNA with a 2.2kb deletion of 3' flanking sequences and a reduction in the size of the largest intron to 356bp is driven by a 388bp fragment of an Hsp70 gene. (Klemenz et al., 1987) Construct: A 'mini-white' gene constructed by deletion of the XbaI-HindII fragment from the long 5' intron. Carried by the W6 and W8 series of vectors.
Phenotypic class	suppressor of variegation, with Su(var)205⁵⁰² (Ahmad and Golic, 1996)
Phenotype manifest in	pigment cell (Kurkulos et al., 1994, Golic and Lindquist, 1989)
Other information
Expression Data
Reporter Expression
distribution deduced from reporter Stage Tissue/Position (including subcellular localization) Reference embryonic stage epidermis (Perrimon et al., 1991) organism \| 10-100% egg length (Perrimon et al., 1991) • nucleus (Perrimon et al., 1991) extended germ band stage dorsal ectoderm \| segmentally repeated (Bellen et al., 1989) embryonic stage 9 -- 12 procephalic neurogenic region (Bellen et al., 1989)
Additional Information
Statement Reference Basal pattern of stripes, restricted to epidermal clusters of cells. Expression localized to nucleus. (Perrimon et al., 1991) Expression pattern dependent upon position of insertion in genome. Basal pattern observed in approximately 30% of transformants: in extended germ band embryos, weak expression in dorsal epidermis, stronger in alternate segments. (Bellen et al., 1989)

Marker for
Reflects expression of	P\T (Bellen et al., 1989)
Progenitors & Descendants
progenitor(s)	pM{MlwB} (Lidholm et al., 1993)
descendant(s)
Comments

Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	M{MlwB} (FlyBase, 1996-) SacW (Lozovsky et al., 2002)
Secondary FlyBase IDs

References ( 5 )
Research paper	Lozovsky et al., 2002, Genetics 160(2): 527--535 Unexpected Stability of mariner Transgenes in Drosophila. [FBrf0144896] Hartl et al., 1997, Genetica 100(1-3): 177--184 Regulation of the transposable element mariner. [FBrf0099809] Lohe and Hartl, 1996, Genetics 143(3): 1299--1306 Reduced germline mobility of a mariner vector containing exogenous DNA: effect of size or site? [FBrf0092616] Lidholm et al., 1993, Genetics 134(3): 859--868 The transposable element mariner mediates germline transformation in Drosophila melanogaster. [FBrf0058610]
Review	Hartl et al., 1997, A. Rev. Genet. 31: 337--358 Modern thoughts on an ancyent marinere: function, evolution, regulation. [FBrf0100570]