Recombinant construct P{UAS-Ras85D.N17}

General Information
Symbol	P{UAS-Ras85D.N17}	FlyBase ID	FBtp0001706
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	1 available
Molecular map
Recent Updates
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FB2013_03
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Lee et al., 1996)
Uses	CV term Qualifiers & info Reference binary system (targeted) Ras85D (Lee et al., 1996) characterization Ras85D (Lee et al., 1996)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference binary component (Lee et al., 1996) chimeric gene (Lee et al., 1996) modified gene (Lee et al., 1996) selectable marker (Lee et al., 1996)
Component Alleles
Allele	Ras85D^N17.Scer\UAS
Reference(s)	(Caldwell et al., 2005, Artero et al., 2001, Alvarez et al., 2003, Cho et al., 2002, Hidalgo et al., 2001, Rangarajan et al., 1999, Kumar and Moses, 2001, Gilboa and Lehmann, 2006, Beckett and Baylies, 2006, Fritz and VanBerkum, 2002, Koh et al., 2002, Sen et al., 2004, Gallio et al., 2004, Dearborn and Kunes, 2004, Urban et al., 2004, Prober and Edgar, 2000, Kumar and Moses, 2001, Duchek and Rorth, 2001, Bruckner et al., 2004, Martin-Bermudo et al., 1999, Harden et al., 1999, Ishimaru et al., 1999, Bajpai et al., 2004, Lee et al., 1996, Kerber et al., 1998, Sepp and Auld, 2003, Boettner et al., 2003, Scholz et al., 1997, Pallavi and Shashidhara, 2003, Scholz et al., 1997, Parker, 2006, Pallavi et al., 2006, Kim et al., 2006, Cela and Llimargas, 2006, Bruckner et al., 2004, Friedman et al., 2011, Terriente-Félix and de Celis, 2009, Rendina et al., 2010, Shen et al., 2009, Buchon et al., 2010, Helman et al., 2011, Lievens et al., 2005, Maeng et al., 2012, Raymond et al., 2004, Freeman et al., 2010)
Molecular data	Construct: Expression of an XhoI-EcoRI fragment of the dominant negative Ras85D gene is influenced by Scer\UAS Scer\GAL4 binding sites. (Lee et al., 1996)
Phenotypic class	decreased cell death \| cell autonomous \| somatic clone, with Scer\GAL4^Act5C.PP (Prober and Edgar, 2000) decreased cell size \| somatic clone, with Scer\GAL4^Act5C.PP (Prober and Edgar, 2000) developmental rate defective \| second instar larval stage, with Scer\GAL4^amn-c651 (Caldwell et al., 2005) increased cell death \| embryonic stage, with Scer\GAL4^en-e16E (Parker, 2006) increased cell size \| embryonic stage, with Scer\GAL4^en-e16E (Parker, 2006) large body \| adult stage, with Scer\GAL4^amn-c651 (Caldwell et al., 2005) large body \| pupal stage, with Scer\GAL4^amn-c651 (Caldwell et al., 2005) large body \| pupal stage, with Scer\GAL4^amn-X8 (Caldwell et al., 2005) lethal, with Scer\GAL4^elav.PLu (Fritz and VanBerkum, 2002) neuroanatomy defective, with Scer\GAL4^futsch-C380 (Koh et al., 2002) neuroanatomy defective \| embryonic stage, with Scer\GAL4^repo (Sepp and Auld, 2003) visible, with Scer\GAL4^bi.PU (Rendina et al., 2010) visible, with Scer\GAL4^Bx-MS1096 (Friedman et al., 2011) visible, with Scer\GAL4^dpp.blk1 (Ishimaru et al., 1999) visible, with Scer\GAL4^ey.PH (Fritz and VanBerkum, 2002) visible, with Scer\GAL4^GMR.PU (Raymond et al., 2004) visible \| somatic clone, with Scer\GAL4^Act5C.PP (Prober and Edgar, 2000)
Phenotype manifest in	adult gut, with Scer\GAL4^{Myo31DF-NP0001} (Buchon et al., 2010) adult gut, with Scer\GAL4^{Myo31DF-NP0001}, Scer\GAL80^ts.αTub84B (Buchon et al., 2010) anterior crossvein, with Scer\GAL4^dpp.blk1 (Ishimaru et al., 1999) border follicle cell, with Scer\GAL4^c306 (Lee et al., 1996) border follicle cell, with Scer\GAL4^slbo.2.6 (Duchek and Rorth, 2001) bouton \| supernumerary, with Scer\GAL4^futsch-C380 (Koh et al., 2002) dMP2 neuron, with Scer\GAL4^elav.PLu (Fritz and VanBerkum, 2002) embryonic/first instar larval cuticle \| dorsal, with Scer\GAL4^hs.PB (Harden et al., 1999) embryonic/larval ganglionic branch, with Scer\GAL4^bs-23.26 (Gallio et al., 2004) embryonic hemocyte, with Scer\GAL4^srp.Hemo (Bruckner et al., 2004) embryonic segment, with Scer\GAL4^en-e16E (Parker, 2006) eye, with Scer\GAL4^ey.PH (Fritz and VanBerkum, 2002) eye, with Scer\GAL4^GMR.PU (Raymond et al., 2004) fascicle \| embryonic stage, with Scer\GAL4^repo (Sepp and Auld, 2003) fusion competent cell, with Scer\GAL4^twi.PB (Beckett and Baylies, 2006) germline stem cell \| supernumerary, with Scer\GAL4^C587 (Gilboa and Lehmann, 2006) glial cell & antennal disc, with Scer\GAL4^hs.PB (Sen et al., 2004) imaginal disc \| somatic clone, with Scer\GAL4^Act5C.PP (Prober and Edgar, 2000) interface glial cell, with Scer\GAL4^{S007708-Jan136} (Hidalgo et al., 2001) larval optic lobe & glial cell \| somatic clone \| cell non-autonomous, with Scer\GAL4^αTub84B.PP (Dearborn and Kunes, 2004) medulla cortex & glial cell \| somatic clone \| cell non-autonomous, with Scer\GAL4^αTub84B.PP (Dearborn and Kunes, 2004) mesothoracic tergum, with Scer\GAL4^{Ubx-lac1-Gal4} (Pallavi and Shashidhara, 2003) mitotic cell cycle \| somatic clone, with Scer\GAL4^Act5C.PP (Prober and Edgar, 2000) morphogenetic furrow, with Scer\GAL4^dpp.blk1 (Kumar and Moses, 2001) motor neuron \| embryonic stage, with Scer\GAL4^repo (Sepp and Auld, 2003) pCC neuron, with Scer\GAL4^elav.PLu (Fritz and VanBerkum, 2002) peripheral glial cell \| embryonic stage, with Scer\GAL4^repo (Sepp and Auld, 2003) photoreceptor cell & axon & eye disc, with Scer\GAL4^bi-omb-Gal4 (Rangarajan et al., 1999) presumptive embryonic/larval peripheral nervous system, with Scer\GAL4^repo (Sepp and Auld, 2003) sensory neuron & axon & embryo, with Scer\GAL4^repo (Sepp and Auld, 2003) subretinal glial cell & eye disc, with Scer\GAL4^bi-omb-Gal4 (Rangarajan et al., 1999) subretinal glial cell & larval optic stalk, with Scer\GAL4^bi-omb-Gal4 (Rangarajan et al., 1999) tracheal branch primordium, with Scer\GAL4^btl.PS (Cela and Llimargas, 2006) vMP2 neuron, with Scer\GAL4^elav.PLu (Fritz and VanBerkum, 2002) wing, with Scer\GAL4^{Ubx-lac1-Gal4} (Pallavi and Shashidhara, 2003) wing disc, with Scer\GAL4^ap-md544 (Pallavi and Shashidhara, 2003) wing disc, with Scer\GAL4^Gug-AGiR (Pallavi and Shashidhara, 2003) wing disc, with Scer\GAL4^tsh-md621 (Pallavi and Shashidhara, 2003) wing disc, with Scer\GAL4^{Ubx-lac1-Gal4} (Pallavi and Shashidhara, 2003) wing vein, with Scer\GAL4^Bx-MS1096 (Friedman et al., 2011) wing vein, with Scer\GAL4^MD-638 (Terriente-Félix and de Celis, 2009) wing vein \| somatic clone, with Scer\GAL4^Act5C.PP (Prober and Edgar, 2000) wing vein L4, with Scer\GAL4^bi.PU (Rendina et al., 2010)
Other information

Allele	w^+mC
Reference(s)	(Lee et al., 1996)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	pP{UAST} (Lee et al., 1996)
descendant(s)
Comments
	construct_comment: Progenitors are those of the plasmid, pP{UAS-Ras85D.N17}. (Lee et al., 1996)
Stocks Listed in FlyBase ( 2 )
Bloomington	4845 P{UAS-Ras85D.N17}TL1, w¹¹¹⁸
Kyoto	107904 P{UAS-Ras85D.N17}TL1, w¹¹¹⁸
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	dnRas (Fritz and VanBerkum, 2002) Dn-RasN17 (Sen et al., 2004) P{UAS-Ras85D.N17} (FlyBase, 1996-, Shen et al., 2009) Ras DN (Shen et al., 2009) UAS-DNras (Alvarez et al., 2003) UAS-Dras1^N17 (Kumar and Moses, 2001, Lee et al., 1996, Kumar and Moses, 2001) UAS-Dras^N17 (Bajpai et al., 2004, Pallavi and Shashidhara, 2003, Pallavi et al., 2006) UASD-ras^N17 (Artero et al., 2001) UASRas1^N17 (Rangarajan et al., 1999, Ishimaru et al., 1999) UAS Ras1^N17 (Friedman et al., 2011) UAS-Ras1N17 (Dearborn and Kunes, 2004, Sepp and Auld, 2003, Harden et al., 1999) UAS-Ras85D.N17 (Lievens et al., 2005, Gilboa and Lehmann, 2006) UAS-Ras85D^N17 (Maeng et al., 2012) UAS-Ras85D DN (Shen et al., 2009) UAS-Ras185DN17 (Koh et al., 2002, Freeman et al., 2010) UAS-Ras^DN (Buchon et al., 2010) UAS-ras^N17 (Hidalgo et al., 2001, Beckett and Baylies, 2006) UAS-Ras^N17 (Prober and Edgar, 2000, Martin-Bermudo et al., 1999, Kim et al., 2006, Cela and Llimargas, 2006, Terriente-Félix and de Celis, 2009, Rendina et al., 2010) UAS-RasN17 (Gallio et al., 2004, Bruckner et al., 2004, Bruckner et al., 2004, Raymond et al., 2004) UAS-rasN17 (Urban et al., 2004) UASt-Ras^N17 (Helman et al., 2011)
Secondary FlyBase IDs

References ( 45 )

Generate a list of	All references relating to this transgenic transposon ( 45 )

List References by type	Research paper ( 43 ) Supplementary material ( 1 ) Erratum ( 1 )
Recent research papers ( 3 )
	Maeng et al., 2012, Mol. Cells 33(5): 457--463 The BTB/POZ-ZF transcription factor dPLZF is involved in Ras/ERK signaling during Drosophila wing development. [FBrf0218378] Friedman et al., 2011, Sci. Signal. 4(196): rs10 Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. [FBrf0216513] Helman et al., 2011, Curr. Biol. 21(13): 1102--1110 Phosphorylation of Groucho Mediates RTK Feedback Inhibition and Prolonged Pathway Target Gene Expression. [FBrf0214255] Show all research papers ...