Recombinant construct P{UAS-pan.dTCF}

General Information
Symbol	P{UAS-pan.dTCF}	FlyBase ID	FBtp0001720
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	2 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (van de Wetering et al., 1997)
Uses	CV term Qualifiers & info Reference binary system (targeted) pan (van de Wetering et al., 1997) characterization pan (van de Wetering et al., 1997)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference binary component (van de Wetering et al., 1997) chimeric gene (van de Wetering et al., 1997) selectable marker (van de Wetering et al., 1997)
Component Alleles
Allele	pan^Scer\UAS.cWa
Reference(s)	(van de Wetering et al., 1997, Kiger et al., 2001, Jackson et al., 2002, Verheyen et al., 2001, Yang et al., 2000, Helms et al., 1999, Tolwinski and Wieschaus, 2001, Cavallo et al., 1998, Waltzer and Bienz, 1998, Chao et al., 2007, Lawrence et al., 2000, Haussmann et al., 2008, Berger et al., 2005, Morris et al., 2008)
Molecular data	Construct: Expression of a fragment of full length pan is directed by Scer\UAS Scer\GAL4 binding sites. (van de Wetering et al., 1997)
Phenotypic class	lethal, with Scer\GAL4^elav-C155 (Jackson et al., 2002) lethal, with Scer\GAL4^hs.2sev (Jackson et al., 2002) neuroanatomy defective \| somatic clone \| third instar larval stage, with Scer\GAL4^elav.PH (Haussmann et al., 2008) partially lethal - majority die \| pupal stage, with Scer\GAL4^dpp.blk1 (Chao et al., 2007) visible, with Scer\GAL4^Bx-MS1096 (Lawrence et al., 2000) visible, with Scer\GAL4^sd-SG29.1 (Lawrence et al., 2000) visible, with Scer\GAL4^sev.EP (Jackson et al., 2002) visible, with Scer\GAL4^vg.int2.1 (Verheyen et al., 2001) wild-type, with Scer\GAL4^e22c (Cavallo et al., 1998)
Phenotype manifest in	denticle belt, with Scer\GAL4^e22c (van de Wetering et al., 1997) embryonic/larval neuromuscular junction \| somatic clone, with Scer\GAL4^elav.PH (Haussmann et al., 2008) interommatidial bristle, with Scer\GAL4^sev.EP (Jackson et al., 2002) NMJ bouton \| somatic clone, with Scer\GAL4^elav.PH (Haussmann et al., 2008) wing, with Scer\GAL4^Bx-MS1096 (Lawrence et al., 2000) wing, with Scer\GAL4^sd-SG29.1 (Lawrence et al., 2000) wing margin, with Scer\GAL4^Bx-MS1096 (Lawrence et al., 2000) wing margin, with Scer\GAL4^sd-SG29.1 (Lawrence et al., 2000) wing margin \| posterior, with Scer\GAL4^vg.int2.1 (Verheyen et al., 2001) wing sensillum \| ectopic, with Scer\GAL4^Bx-MS1096 (Lawrence et al., 2000) wing vein \| ectopic, with Scer\GAL4^Bx-MS1096 (Lawrence et al., 2000)
Other information

Allele	w^+mC
Reference(s)	(van de Wetering et al., 1997)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	pP{UAST} (van de Wetering et al., 1997)
descendant(s)
Comments
	construct_comment: Progenitors are those of the plasmid, pP{UAS-pan.dTCF}. (van de Wetering et al., 1997)
Stocks Listed in FlyBase ( 4 )
Bloomington	4838 y¹ w¹¹¹⁸; P{UAS-pan.dTCF}4 4837 y¹ w¹¹¹⁸; P{UAS-pan.dTCF}24/CyO
Kyoto	107900 y¹ w¹¹¹⁸; P{UAS-pan.dTCF}4 107899 y¹ w¹¹¹⁸; P{UAS-pan.dTCF}24/CyO
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	GAL4-regulated dTCF (van de Wetering et al., 1997) P{UAS-pan.dTCF} (FlyBase, 1996-) P{UAS-pan.TCF} (FlyBase, 1996-) P{w^+mC = UAS-pan.dTCF} (Haussmann et al., 2008) UAS-dTCF (Jackson et al., 2002, Tolwinski and Wieschaus, 2001, Helms et al., 1999, Morris et al., 2008, Berger et al., 2005) UASdTcf (Yang et al., 2000, Lawrence et al., 2000) UAS-dTCFwt (Verheyen et al., 2001) UAS-pan (Kiger et al., 2001, Haussmann et al., 2008) UAS-Tcf (Chao et al., 2007)
Secondary FlyBase IDs

References ( 14 )
Research paper	Haussmann et al., 2008, Genome Biol. 9(4): R73 Erect wing regulates synaptic growth in Drosophila by integration of multiple signaling pathways. [FBrf0209726] Morris et al., 2008, Nature 455(7212): 552--556 E2F1 represses beta-catenin transcription and is antagonized by both pRB and CDK8. [FBrf0221005] Chao et al., 2007, Development 134(5): 989--997 The HMG-box transcription factor SoxNeuro acts with Tcf to control Wg/Wnt signaling activity. [FBrf0195251] Berger et al., 2005, Hum. Mol. Genet. 14(20): 3003--3011 Lithium rescues toxicity of aggregate-prone proteins in Drosophila by perturbing Wnt pathway. [FBrf0190825] Jackson et al., 2002, Neuron 34(4): 509--519 Human wild-type tau interacts with wingless pathway components and produces neurofibrillary pathology in Drosophila. [FBrf0149139] Kiger et al., 2001, Proc. Natl. Acad. Sci. U.S.A. 98(18): 10190--10195 Hemocytes are essential for wing maturation in Drosophila melanogaster. [FBrf0138518] Tolwinski and Wieschaus, 2001, Development 128(11): 2107--2117 Armadillo nuclear import is regulated by cytoplasmic anchor Axin and nuclear anchor dTCF/Pan. [FBrf0136754] Verheyen et al., 2001, Mech. Dev. 101(1-2): 119--132 The tissue polarity gene nemo carries out multiple roles in patterning during Drosophila development. [FBrf0134745] Lawrence et al., 2000, Int. J. Dev. Biol. 44(7): 749--756 dTcf antagonises Wingless signalling during the development and patterning of the wing in Drosophila. [FBrf0132357] Yang et al., 2000, Development 127(17): 3695--3702 decapentaplegic is a direct target of dTcf repression in the Drosophila visceral mesoderm. [FBrf0130179] Helms et al., 1999, Dev. Biol. 215(2): 358--374 Engineered truncations in the Drosophila mastermind protein disrupt Notch pathway function. [FBrf0111904] Cavallo et al., 1998, Nature 395(6702): 604--608 Drosophila Tcf and Groucho interact to repress Wingless signalling activity. [FBrf0104944] Waltzer and Bienz, 1998, Nature 395(6701): 521--525 Drosophila CBP represses the transcription factor TCF to antagonize Wingless signalling. [FBrf0104794] van de Wetering et al., 1997, Cell 88(6): 789--799 Armadillo coactivates transcription driven by the product of the Drosophila segment polarity gene dTCF. [FBrf0093264]