Recombinant construct P{GAL4-Eh.2.4}

General Information
Symbol	P{GAL4-Eh.2.4}	FlyBase ID	FBtp0001739
Feature type	transgenic_transposon	Created / Updated	1999-02-04/2001-12-17

Size		Expression data	GAL4 reporter/driver
Location-dependent role		Associated insertions	2 available
Molecular map
Description & Uses
Species	synthetic construct
Description	CV term Qualifiers & info Reference transposon P-element (McNabb et al., 1997)
Uses	CV term Qualifiers & info Reference binary system (regulatory) Eh (McNabb et al., 1997) characterization Eh \| regulatory (McNabb et al., 1997)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Sequence
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference binary component (McNabb et al., 1997) chimeric gene (McNabb et al., 1997) selectable marker (McNabb et al., 1997) reporter gene (McNabb et al., 1997)
Component Alleles
w^+mC
Reference(s)	(McNabb et al., 1997)
Molecular data	Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors. 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Minigene of the white cDNA. (Chung et al., 1993) Deletion of w^+mC 5' flanking sequences to position -305. (Qian and Pirrotta, 1995)
Phenotypic class	courtship defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^Sym.Scer\UAS, w^{a.Sym.Scer\UAS} (Giordano et al., 2002)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^Sym.Scer\UAS, w^{a.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Scer\GAL4^Eh.2.4
Reference(s)	(McNabb et al., 1997, Clark et al., 2004, Clark et al., 2002, Baker et al., 1999, Kim et al., 2006)
Molecular data	Construct: A 2.4kb SalI-XbaI fragment including the Eh transcriptional start site and extending upstream from it, is fused upstream of Scer\GAL4. (McNabb et al., 1997)
Phenotypic class	cell death defective, with W^Scer\UAS.cZa (McNabb et al., 1997) semi-lethal \| larval stage, with W^Scer\UAS.cZa (McNabb et al., 1997) cell death defective, with rpr^Scer\UAS.cZa (McNabb et al., 1997) semi-lethal \| larval stage, with rpr^Scer\UAS.cZa (McNabb et al., 1997) eclosion rhythm defective, with rpr^Scer\UAS.cZa (McNabb et al., 1997) cell death increase, with rpr^Scer\UAS.cZa (Clark et al., 2004)
Phenotype manifest in	adult cuticle, with nos^{αTub84B.3'UTR.nos+2.Scer\UAS} (Clark et al., 2002) wing, with nos^{αTub84B.3'UTR.nos+2.Scer\UAS} (Clark et al., 2002) embryonic/larval trachea \| dorsal, with W^Scer\UAS.cZa (McNabb et al., 1997) neuron & larva, with W^Scer\UAS.cZa (McNabb et al., 1997) adult cuticle, with nos^{αTub84B.3'UTR.Scer\UAS} (Clark et al., 2002) wing, with nos^{αTub84B.3'UTR.Scer\UAS} (Clark et al., 2002) embryonic/larval trachea \| dorsal, with rpr^Scer\UAS.cZa (McNabb et al., 1997) neuron & larva, with rpr^Scer\UAS.cZa (McNabb et al., 1997) adult cuticle, with rpr^Scer\UAS.cZa (McNabb et al., 1997) wing, with rpr^Scer\UAS.cZa (McNabb et al., 1997) EH neurosecretory neuron, with rpr^Scer\UAS.cZa (Kim et al., 2006, Clark et al., 2004) larval mouth, with rpr^Scer\UAS.cZa (Clark et al., 2004) larval mouth, with Scer\GAL4^Ccap.PP, rpr^Scer\UAS.cZa (Clark et al., 2004)
Other information
Expression Data
Reporter Expression
Stage Tissue/Position Reference embryonic stage \| stage 17-L \| third instar stage 2 neuron \| subset (McNabb et al., 1997) embryonic stage \| stage 17-L \| third instar stage 2 neuron <of> brain \| subset (McNabb et al., 1997) larval stage-adult stage neuron <of> brain \| subset (Clark et al., 2002) pupal stage central nervous system \| restricted (Baker et al., 1999) pupal stage neuron <of> central nervous system \| subset (Baker et al., 1999) embryonic stage \| stage 17-L \| third instar stage 2 brain \| restricted (McNabb et al., 1997) larval stage-adult stage neuron \| subset (Clark et al., 2002) pupal stage neuron \| subset (Baker et al., 1999) larval stage-adult stage brain \| restricted (Clark et al., 2002)
Additional Information
Statement Reference Expression is observed in eclosion hormone-expressing neurons. (Baker et al., 1999) Expression is observed in eclosion-hormone expressing neurons. (Clark et al., 2002) Late third instar larval expression is observed in two neurons, the eclosion hormone neurons, in the brain lobes. A representative image can be seen in FBrf0098847. (McNabb et al., 1997)

Assay mode	distribution deduced from reporter (Gal4 UAS) (McNabb et al., 1997) distribution deduced from reporter (Gal4 UAS) (McNabb et al., 1997) distribution deduced from reporter (Gal4 UAS) (Clark et al., 2002) distribution deduced from reporter (Gal4 UAS) (Baker et al., 1999) distribution deduced from reporter (Gal4 UAS) (Baker et al., 1999) distribution deduced from reporter (Gal4 UAS) (McNabb et al., 1997) distribution deduced from reporter (Gal4 UAS) (Clark et al., 2002) distribution deduced from reporter (Gal4 UAS) (Baker et al., 1999) distribution deduced from reporter (Gal4 UAS) (Clark et al., 2002)
Marker for	embryonic/larval brain neuron \| subset (McNabb et al., 1997)
Reflects expression of	Eh (McNabb et al., 1997, Baker et al., 1999, Clark et al., 2002)
Progenitors & Descendants
progenitor(s)	pGaTB pP{CaSpeR-4} (McNabb et al., 1997, McNabb et al., 1997)
descendant(s)
Comments
	construct_comment: Progenitors are those of the plasmid, pP{GAL4-Eh.2.4}. (McNabb et al., 1997)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	EH-GAL4 (Clark et al., 2004) EHup-GAL4 (Kim et al., 2006) EHups-GAL4 (Clark et al., 2002, McNabb et al., 1997, Baker et al., 1999) P{@w^+mC@ @GAL4^Eh.2.4@=@GAL4-Eh.2.4@} (FlyBase, 1996-) P{GAL4-Eh.2.4} (FlyBase, 1996-)
Secondary FlyBase IDs

References ( 17 )
Research paper	Kim et al., 2006, Curr. Biol. 16(13): 1395--1407 A command chemical triggers an innate behavior by sequential activation of multiple peptidergic ensembles. [FBrf0194317] Clark et al., 2004, J. Neurosci. 24(17): 4283--4292 Neuroendocrine control of larval ecdysis behavior in Drosophila: complex regulation by partially redundant neuropeptides. [FBrf0174859] Clark et al., 2002, Development 129(14): 3325--3334 A common translational control mechanism functions in axial patterning and neuroendocrine signaling in Drosophila. [FBrf0151259] Giordano et al., 2002, Genetics 160(2): 637--648 RNAi triggered by symmetrically transcribed transgenes in Drosophila melanogaster. [FBrf0144904] Lloyd et al., 2002, Genome 45(2): 296--312 Enhancer of garnet/δAP-3 is a cryptic allele of the white gene and identifies the intracellular transport system for the white protein. [FBrf0147069] Baker et al., 1999, J. exp. Biol. 202(21): 3037--3048 The hormonal coordination of behavior and physiology at adult ecdysis in Drosophila melanogaster. [FBrf0111791] Soldatov et al., 1999, Molec. Cell. Biol. 19(5): 3769--3778 TAFII40 protein is encoded by the e(y)1 gene: biological consequences of mutations. [FBrf0108279] McNabb et al., 1997, Neuron 19(4): 813--823 Disruption of a behavioral sequence by targeted death of peptidergic neurons in Drosophila. [FBrf0098847] Hagstrom et al., 1996, Genes Dev. 10(24): 3202--3215 Fab-7 functions as a chromatin domain boundary to ensure proper segment specification by the Drosophila bithorax complex. [FBrf0091065] Hing and Carlson, 1996, J. Neurobiol. 30(4): 454--464 Male-male courtship behavior induced by ectopic expression of the Drosophila white gene: Role of sensory function and age. [FBrf0089680] True et al., 1996, Genetics 142(3): 819--837 A genome-wide survey of hybrid incompatibility factors by the introgression of marked segments of Drosophila mauritiana chromosomes into Drosophila simulans. [FBrf0086664] Qian and Pirrotta, 1995, Genetics 139(2): 733--744 Dosage compensation of the Drosophila white gene requires both the X chromosome environment and multiple intragenic elements. [FBrf0080335] Dorer and Henikoff, 1994, Cell 77(7): 993--1002 Expansions of transgene repeats cause heterochromatin formation and gene silencing in Drosophila. [FBrf0072979] Chung et al., 1993, Cell 74(3): 505--514 A 5' element of the chicken -globin domain serves as an insulator in human erythroid cells and protects against position effect in Drosophila. [FBrf0064408] Roseman et al., 1993, EMBO J. 12(2): 435--442 The su(Hw) protein insulates expression of the Drosophila melanogaster white gene from chromosomal position-effects. [FBrf0058161]
Review	Pirrotta, 1988, Rodriguez, Denhardt, 1988: 437--456 Vectors for P-mediated transformation in Drosophila. [FBrf0048982]
FlyBase analysis	FlyBase, 1996-, FlyBase inference based on genome sequence analysis. FlyBase inference based on genome sequence analysis. [FBrf0104946]