Recombinant construct P{UAS-Fas2.PEST+}

General Information
Symbol	P{UAS-Fas2.PEST+}	FlyBase ID	FBtp0002642
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	0 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Lin, 1997.11.4)
Uses	CV term Qualifiers & info Reference binary system (targeted) Fas2 (Lin, 1997.11.4)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference binary component (Davis et al., 1997) binary component (Lin, 1997.11.4) chimeric gene (Lin, 1997.11.4) selectable marker (Lin, 1997.11.4)
Component Alleles
Allele	Fas2^Scer\UAS.cLb
Reference(s)	(Davis et al., 1997, Davis and Goodman, 1998, Baines et al., 2002, Lin, 1997.11.4, Winberg et al., 1998, Kristiansen et al., 2005, Banovic et al., 2010, Beck et al., 2012, Mosca et al., 2012)
Molecular data	Construct: Expression of a PEST⁺ transmembrane isoform of Fas2 is governed by Scer\UAS regulatory sequences. (Davis et al., 1997) Expression of a Fas2 PEST+ cDNA (with the ends of the cDNA modified to remove all 5' and 3' untranslated sequences) is governed by Scer\UAS regulatory sequences. (Lin, 1997.11.4)
Phenotypic class	neuroanatomy defective, with Scer\GAL4^B19, Scer\GAL4^eve.RRK (Baines et al., 2002) neuroanatomy defective, with Scer\GAL4^B185 (Davis et al., 1997) neuroanatomy defective, with Scer\GAL4^elav.PLu (Davis et al., 1997) neuroanatomy defective, with Scer\GAL4^eve.RRK (Baines et al., 2002) neuroanatomy defective, with Scer\GAL4^how-24B (Davis et al., 1997) neuroanatomy defective, with Scer\GAL4^{l(2)E105-E105} (Davis et al., 1997) neuroanatomy defective, with Scer\GAL4^l(3)H94-H94 (Davis et al., 1997, Davis and Goodman, 1998) neuroanatomy defective, with Scer\GAL4^Mhc.PW (Davis et al., 1997) neuroanatomy defective, with Scer\GAL4^MS1075 (Kristiansen et al., 2005) neurophysiology defective, with Scer\GAL4^B19 (Baines et al., 2002) neurophysiology defective, with Scer\GAL4^eve.RRK (Baines et al., 2002)
Phenotype manifest in	bouton, with Scer\GAL4^B185 (Davis et al., 1997) bouton, with Scer\GAL4^elav.PLu (Davis et al., 1997) bouton, with Scer\GAL4^how-24B (Davis et al., 1997) bouton, with Scer\GAL4^{l(2)E105-E105} (Davis et al., 1997) bouton, with Scer\GAL4^l(3)H94-H94 (Davis et al., 1997) bouton, with Scer\GAL4^Mhc.PW (Davis et al., 1997) mechanosensory neuron, with Scer\GAL4^MS1075 (Kristiansen et al., 2005) ocellar nerve, with Scer\GAL4^MS1075 (Kristiansen et al., 2005) RP1 motor neuron, with Scer\GAL4^l(3)H94-H94 (Davis and Goodman, 1998) RP3 motor neuron, with Scer\GAL4^l(3)H94-H94 (Davis and Goodman, 1998) RP5 motor neuron, with Scer\GAL4^l(3)H94-H94 (Davis and Goodman, 1998) synapse, with Scer\GAL4^l(3)H94-H94 (Davis and Goodman, 1998) synapse & aCC neuron, with Scer\GAL4^B19 (Baines et al., 2002) synapse & aCC neuron, with Scer\GAL4^eve.RRK (Baines et al., 2002) synapse & RP2 neuron, with Scer\GAL4^B19 (Baines et al., 2002) synapse & RP2 neuron, with Scer\GAL4^eve.RRK (Baines et al., 2002) synapse & RP3 neuron, with Scer\GAL4^B19, Scer\GAL4^eve.RRK (Baines et al., 2002) synapse \| ectopic, with Scer\GAL4^B185 (Davis et al., 1997) synapse \| ectopic, with Scer\GAL4^elav.PLu (Davis et al., 1997) synapse \| ectopic, with Scer\GAL4^how-24B (Davis et al., 1997) synapse \| ectopic, with Scer\GAL4^{l(2)E105-E105} (Davis et al., 1997) synapse \| ectopic, with Scer\GAL4^l(3)H94-H94 (Davis et al., 1997, Davis and Goodman, 1998) synapse \| ectopic, with Scer\GAL4^Mhc.PW (Davis et al., 1997) transverse nerve, with Scer\GAL4^l(3)H94-H94 (Winberg et al., 1998)
Other information

Allele	w^+mC
Reference(s)	(Lin, 1997.11.4)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	pP{UAST} (Lin, 1997.11.4)
descendant(s)
Comments
	construct_comment: Progenitors are those of the plasmid, pP{UAS-Fas2.PEST+}. (Lin, 1997.11.4)
Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	FasII (PEST+) (Baines et al., 2002) FasII^PEST+ (Kristiansen et al., 2005) P{UAS-Fas2.PEST+} (FlyBase, 1996-) UAS-Fas2 (Mosca et al., 2012) UAS-fasII (Banovic et al., 2010) UAS-FasII-A-PEST+ (Beck et al., 2012) UAS-FasII-PEST+ (Beck et al., 2012) UAST-FasII (PEST+) (Lin, 1997.11.4)
Secondary FlyBase IDs

References ( 9 )
Research paper	Beck et al., 2012, J. Neurosci. 32(20): 7058--7073 Regulation of Fasciclin II and Synaptic Terminal Development by the Splicing Factor Beag. [FBrf0218385] Banovic et al., 2010, Neuron 66(5): 724--738 Drosophila Neuroligin 1 Promotes Growth and Postsynaptic Differentiation at Glutamatergic Neuromuscular Junctions. [FBrf0211035] Kristiansen et al., 2005, Mol. Cell. Neurosci. 28(1): 141--152 Genetic analysis of an overlapping functional requirement for L1- and NCAM-type proteins during sensory axon guidance in Drosophila. [FBrf0184161] Baines et al., 2002, J. Neurosci. 22(15): 6587--6595 Regulation of synaptic connectivity: levels of Fasciclin II influence synaptic growth in the Drosophila CNS. [FBrf0151382] Davis and Goodman, 1998, Nature 392(6671): 82--86 Synapse-specific control of synaptic efficacy at the terminals of a single neuron. [FBrf0100746] Winberg et al., 1998, Cell 93(4): 581--591 Genetic analysis of the mechanisms controlling target selection: complementary and combinatorial functions of netrins, semaphorins, and IgCAMs. [FBrf0102605] Davis et al., 1997, Neuron 19(3): 561--573 Genetic analysis of the mechanisms controlling target selection: target-derived Fasciclin II regulates the pattern of synapse formation. [FBrf0098766]
Supplementary material	Mosca et al., 2012, Nature 484(7393): Online methods. [FBrf0219881]
Personal communication to FlyBase	Lin, 1997.11.4, UAS-Fas2 and UAS-lacZ. UAS-Fas2 and UAS-lacZ. [FBrf0099039]