Recombinant construct P{tubP-GAL80}

General Information
Symbol	P{tubP-GAL80}	FlyBase ID	FBtp0002650
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	7 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Lee and Luo, 1999)
Uses	CV term Qualifiers & info Reference analytical tool mosaic analysis (Lee and Luo, 1999)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference chimeric gene (Lee and Luo, 1999) general promoter (Lee and Luo, 1999) reporter gene (Lee and Luo, 1999) selectable marker (Lee and Luo, 1999)
Component Alleles
Allele	Scer\GAL80^αTub84B.PL
Reference(s)	(Casal et al., 2006, Bello et al., 2003, Hayashi and Carthew, 2004, Brumby et al., 2002, Grueber et al., 2002, Okajima, 2005, Lee et al., 1999, Pappu et al., 2003, Janody et al., 2003, Jefferis et al., 2001, Saucedo et al., 2003, Marin et al., 2002, Wang and Struhl, 2005, Inoshita and Tanimura, 2006, Zhu et al., 2003, Zhu et al., 2006, Clark et al., 2002, Reuter et al., 2003, Komiyama et al., 2003, Casal et al., 2002, Noureddine et al., 2002, Duffy, 2002, Sweeney et al., 2002, Nicolai et al., 2003, Zelhof and Hardy, 2004, Zhu and Luo, 2004, Rolls et al., 2003, Moore et al., 2004, Iyengar et al., 2006, Jhaveri et al., 2004, Awasaki and Ito, 2004, Jefferis et al., 2004, Baena-Lopez et al., 2003, Pagliarini and Xu, 2003, Lei et al., 2003, Martini et al., 2000, Lee et al., 2000, Luo et al., 1999, Grewal et al., 2005, Lee and Luo, 1999, Liu et al., 2000, Truman et al., 2004, Lee and Treisman, 2001, Grueber et al., 2003, Sasamura et al., 2003, Vef et al., 2006, Long et al., 2006, Poulton and Deng, 2006, Baena-Lopez and Garcia-Bellido, 2006, Komiyama et al., 2004, Wang and Struhl, 2004, Sweeney et al., 2006, Oh and Irvine, 2009, Lawrence et al., 2004, Zhu et al., 2005, Miura et al., 2006, Mondal et al., 2007, Zhang et al., 2011, Awasaki et al., 2008, Hung et al., 2011, Grueber et al., 2007, Zhang et al., 2012, Stephan et al., 2011, Cabernard and Affolter, 2005, Thompson et al., 2005, Lai and Lee, 2006, Tien et al., 2008, Bischoff and Cseresnyés, 2009, Sousa-Nunes et al., 2009, Potter et al., 2010, Potter et al., 2010, Takashima et al., 2008, Lai et al., 2008, Sweeney et al., 2007, Franciscovich et al., 2008, Blanco et al., 2010, Cabernard and Doe, 2009, Betz et al., 2008, Dimitrova et al., 2008, Raghu et al., 2007, Cachero et al., 2010, Scott et al., 2002, Rhiner et al., 2010, Yu et al., 2010, White et al., 2010, Chiang et al., 2011, Laplante and Nilson, 2011, Dow et al., 2003, Orihara-Ono et al., 2011, Zeng et al., 2010, Varija Raghu et al., 2011, Molnar et al., 2011, Poulton et al., 2011, Hasegawa et al., 2011, Viktorin et al., 2011, Leonardi et al., 2011, Moraru et al., 2012, Crocker et al., 2010, Roch et al., 2010, Biteau et al., 2010, Mathur et al., 2010, Herranz et al., 2010, Hudson and Cooley, 2010)
Molecular data	Construct: Expression of Scer\GAL80 is controlled by the αTub84B promoter. (Lee and Luo, 1999)
Phenotypic class	cell polarity defective \| conditional \| somatic clone, with Ras85D^V12.Scer\UAS, Scer\GAL4^{Scer\FRT.Act5C} (Zeng et al., 2010) cell polarity defective \| conditional \| somatic clone, with Scer\GAL4^{Scer\FRT.Act5C}, phl^{Scer\UAS.F179} (Zeng et al., 2010) fertile (Lee and Luo, 1999) hyperplasia \| conditional \| somatic clone, with Ras85D^V12.Scer\UAS, Scer\GAL4^{Scer\FRT.Act5C} (Zeng et al., 2010) hyperplasia \| conditional \| somatic clone, with Scer\GAL4^{Scer\FRT.Act5C}, phl^{Scer\UAS.F179} (Zeng et al., 2010) lethal, with Scer\GAL4^elav.PLu, W^Scer\UAS.cZa (Mondal et al., 2007) lethal \| temperature conditional, with Scer\GAL4^R15W.elav, W^Scer\UAS.cZa (Mondal et al., 2007) sleep defective, with Bhal\NaChBac^{Scer\UAS.T:Avic\GFP-EGFP}, Scer\GAL4^Tdc2.PC (Crocker et al., 2010) viable (Lee and Luo, 1999) visible, with Scer\GAL4^GMR.PF, yki^{S111A.S168A.S250A.Scer\UAS.T:SV5\V5} (Oh and Irvine, 2009) visible, with Scer\GAL4^GMR.PF, yki^{S168A.Scer\UAS.T:SV5\V5} (Oh and Irvine, 2009)
Phenotype manifest in	adult Malpighian tubule \| conditional \| somatic clone, with Ras85D^V12.Scer\UAS, Scer\GAL4^{Scer\FRT.Act5C} (Zeng et al., 2010) adult Malpighian tubule \| conditional \| somatic clone, with Scer\GAL4^{Scer\FRT.Act5C}, aPKC^{CAAXWT.Scer\UAS} (Zeng et al., 2010) adult Malpighian tubule \| conditional \| somatic clone, with Scer\GAL4^{Scer\FRT.Act5C}, aPKC^ΔN.Scer\UAS (Zeng et al., 2010) adult Malpighian tubule \| conditional \| somatic clone, with Scer\GAL4^{Scer\FRT.Act5C}, phl^{Scer\UAS.F179} (Zeng et al., 2010) eye, with Scer\GAL4^GMR.PF, yki^{S111A.S168A.S250A.Scer\UAS.T:SV5\V5} (Oh and Irvine, 2009) eye, with Scer\GAL4^GMR.PF, yki^{S168A.Scer\UAS.T:SV5\V5} (Oh and Irvine, 2009) eye \| temperature conditional, with Scer\GAL4^R15W.elav, W^Scer\UAS.cZa (Mondal et al., 2007)
Other information

Allele	w^+mC
Reference(s)	(Lee and Luo, 1999)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	pP{CaSpeR-4} (Lee and Luo, 1999) pP{αTub84B-lacZ(-157/+696)} (Lee and Luo, 1999)
descendant(s)
Comments
	construct_comment: For use with the MARCM mosaic system, which allows visualization of mutant cells in conjunction with the GAL4-UAS system; GAL80, which represses activity of GAL4, is absent in FRT-FLP induced clones.Progenitors are those of the plasmid, pP{tubP-GAL80}. (Lee and Luo, 1999)
Stocks Listed in FlyBase ( 36 )
Bloomington	5132 P{hsFLP}1, P{tubP-GAL80}LL1 w^* P{neoFRT}19A 5140 w^; P{FRT(w^hs)}G13 P{tubP-GAL80}LL2 5135 y¹ w^; P{neoFRT}82B P{tubP-GAL80}LL3 5191 y¹ w^; P{tubP-GAL80}LL9 P{neoFRT}80B 5192 y¹ w^; P{tubP-GAL80}LL10 P{neoFRT}40A/CyO 5142 w^; P{FRT(w^hs)}G13 P{πM}45F P{tubP-GAL80}LL2/CyO 42717 y¹ P{tubP-GAL80}LL1 w^ P{ey-FLP.N}2 P{neoFRT}19A
Kyoto	108063 P{neoFRT}19A, P{tubP-GAL80}LL1, P{hsFLP}1, w^* 108066 y¹ w^; P{neoFRT}82B P{tubP-GAL80}LL3 108093 y¹ w^; P{tubP-GAL80}LL9 P{neoFRT}80B 108071 w^; P{FRT(w^hs)}G13 P{tubP-GAL80}LL2/CyO 108094 y¹ w^; P{tubP-GAL80}LL10 P{neoFRT}40A/CyO
	More stocks available...
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{tub-Gal80} (Iyengar et al., 2006) P{tubP-GAL80} (FlyBase, 1996-, Crocker et al., 2010) P{w[+mC]=tubP-GAL80} (Vef et al., 2006) P^tub-Gal80 (Hudson and Cooley, 2010) Tbg-GAL80 (Brumby et al., 2002) tub::Gal80 (Varija Raghu et al., 2011) tub.Gal80 (Casal et al., 2002, Lawrence et al., 2004, Casal et al., 2006, Bischoff and Cseresnyés, 2009) Tub-GAL4 (Hipfner and Cohen, 2003) tub-Gal80 (Okajima, 2005, Grewal et al., 2005, Grueber et al., 2002, Molnar et al., 2011, Oh and Irvine, 2009, Betz et al., 2008, Dimitrova et al., 2008, Rhiner et al., 2010, Zhang et al., 2012, Zeng et al., 2010, Poulton et al., 2011, Hasegawa et al., 2011, Viktorin et al., 2011, Herranz et al., 2010, Moraru et al., 2012) tub-GAL80 (Hayashi and Carthew, 2004, Zelhof and Hardy, 2004, Saucedo et al., 2003, Pappu et al., 2003, Janody et al., 2003, Lee and Treisman, 2001, Tien et al., 2008, Zhang et al., 2011, Takashima et al., 2008, Franciscovich et al., 2008, Leonardi et al., 2011) Tub-Gal80 (Jhaveri et al., 2004, Pagliarini and Xu, 2003, Laplante and Nilson, 2011) Tub-GAL80 (Long et al., 2006, Miura et al., 2006, Leonardi et al., 2011) tubGAL80 (Poulton and Deng, 2006, Mathur et al., 2010) tubgal80 (Cabernard and Affolter, 2005) tubGal80 (Biteau et al., 2010) tub GAL80 (Mathur et al., 2010) tubP-Gal80 (Inoshita and Tanimura, 2006, Zhu and Luo, 2004, Grueber et al., 2003, Sweeney et al., 2006, Sweeney et al., 2007, Grueber et al., 2007, Cabernard and Doe, 2009, Raghu et al., 2007, White et al., 2010, Viktorin et al., 2011, Stephan et al., 2011) tubP-GAL80 (Truman et al., 2004, Awasaki and Ito, 2004, Jefferis et al., 2004, Sasamura et al., 2003, Zhu et al., 2003, Reuter et al., 2003, Komiyama et al., 2003, Clark et al., 2002, Sweeney et al., 2002, Marin et al., 2002, Jefferis et al., 2001, Liu et al., 2000, Martini et al., 2000, Lee et al., 2000, Lee et al., 1999, Lee and Luo, 1999, Lai and Lee, 2006, Scott et al., 2002, Zhu et al., 2006, Zhu et al., 2005, Sousa-Nunes et al., 2009, Potter et al., 2010, Potter et al., 2010, Lai et al., 2008, Blanco et al., 2010, Cachero et al., 2010, Yu et al., 2010, Chiang et al., 2011, Awasaki et al., 2008) tubP-gal80 (Moore et al., 2004) TubP-Gal80 (Komiyama et al., 2004) tubP-GAL80^LL10 (Blanco et al., 2010) tubulin-GAL80^LL1 (Roch et al., 2010) tubulin-GAL80^LL2 (Roch et al., 2010) Tubulin α1-Gal80 (Baena-Lopez and Garcia-Bellido, 2006) Tubα1-G80 (Wang and Struhl, 2005, Wang and Struhl, 2004) tubα1-Gal80 (Thompson et al., 2005) unnamed (Orihara-Ono et al., 2011)
Secondary FlyBase IDs

References ( 104 )

Generate a list of	All references relating to this transgenic transposon ( 104 )

List References by type	Research paper ( 99 ) Supplementary material ( 4 ) Review ( 1 )
Recent research papers ( 14 )
	Moraru et al., 2012, Neural Dev. 7(1): 14 Analysis of cell identity, morphology, apoptosis and mitotic activity in a primary neural cell culture system in Drosophila. [FBrf0218691] Zhang et al., 2012, PLoS ONE 7(2): e31994 Control of tissue growth and cell transformation by the salvador/warts/hippo pathway. [FBrf0217552] Chiang et al., 2011, Curr. Biol. 21(1): 1--11 Three-Dimensional Reconstruction of Brain-wide Wiring Networks in Drosophila at Single-Cell Resolution. [FBrf0212704] Hasegawa et al., 2011, Development 138(5): 983--993 Concentric zones, cell migration and neuronal circuits in the Drosophila visual center. [FBrf0213020] Hung et al., 2011, Science 334(6063): 1710--1713 Direct redox regulation of F-actin assembly and disassembly by Mical. [FBrf0217252] Laplante and Nilson, 2011, J. Cell Biol. 192(2): 335--348 Asymmetric distribution of Echinoid defines the epidermal leading edge during Drosophila dorsal closure. [FBrf0212838] Leonardi et al., 2011, Development 138(16): 3569--3578 Multiple O-glucosylation sites on Notch function as a buffer against temperature-dependent loss of signaling. [FBrf0214548] Molnar et al., 2011, PLoS Genet. 7(3): e1001335 Role of the Drosophila non-visual ß-arrestin kurtz in hedgehog signalling. [FBrf0213301] Orihara-Ono et al., 2011, Dev. Biol. 351(1): 163--175 Downregulation of Notch mediates the seamless transition of individual Drosophila neuroepithelial progenitors into optic medullar neuroblasts during prolonged G1. [FBrf0212978] Poulton et al., 2011, Development 138(9): 1737--1745 The microRNA pathway regulates the temporal pattern of Notch signaling in Drosophila follicle cells. [FBrf0213494] Stephan et al., 2011, Mol. Biol. Cell 22(21): 4079--4092 Membrane-targeted WAVE mediates photoreceptor axon targeting in the absence of the WAVE complex in Drosophila. [FBrf0216499] Varija Raghu et al., 2011, J. Comp. Neurol. 519(1): 162--176 Neurons with cholinergic phenotype in the visual system of Drosophila. [FBrf0212356] Viktorin et al., 2011, Dev. Biol. 356(2): 553--565 Multipotent neural stem cells generate glial cells of the central complex through transit amplifying intermediate progenitors in Drosophila brain development. [FBrf0214495] Zhang et al., 2011, Cell Death Differ. 18(8): 1346--1355 Wbp2 cooperates with Yorkie to drive tissue growth downstream of the Salvador-Warts-Hippo pathway. [FBrf0214266] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...