Recombinant construct P{UAS-lacZ.btau.YES}

General Information
Symbol	P{UAS-lacZ.btau.YES}	FlyBase ID	FBtp0002653
Feature type	transgenic_transposon

Size		Expression data	lacZ reporter
Associated insertions	1 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Lee and Luo, 1999)
Uses	CV term Qualifiers & info Reference binary system (targeted) lacZ (Lee and Luo, 1999) characterization Scer\UAS (Lee and Luo, 1999) reporter construct Ecol\lacZ \| binary system (Lee and Luo, 1999) subcellular target microtubules (Lee and Luo, 1999)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference binary component (Lee and Luo, 1999) chimeric gene (Lee and Luo, 1999) molecular tag (Lee and Luo, 1999) selectable marker (Lee and Luo, 1999)
Component Alleles
Allele	Ecol\lacZ^{Scer\UAS.O.T:Btau\MAPT}
Reference(s)	(Couch et al., 2004, Hitier et al., 2000, Simpson et al., 2000, Hassan et al., 2000, van Meyel et al., 2000, Lee and Luo, 1999, Battye et al., 1999, Srahna et al., 2006, Suster et al., 2003, Gao et al., 2000)
Molecular data	The expression of a Btau\MAPT-Ecol\lacZ fusion gene is governed by Scer\UAS regulatory sequences. (Lee and Luo, 1999) Scer\UAS regulatory sequences drive expression of a Btau\MAPT-Ecol\lacZ fusion gene. (O'Keefe et al., 1998) Construct: 5.4kb EcoRI fragment containing a Btau\MAPT-Ecol\lacZ fusion gene is expressed under the control of Scer\UAS regulatory sequences. (Callahan et al., 1996)
Phenotypic class
Phenotype manifest in	prothoracic neuromere, with Scer\GAL4^smid-C161 (Williams et al., 2000)
Other information

Allele	y^+mDint2
Reference(s)	(Lee and Luo, 1999)
Molecular data	Construct: 5.2kb y⁺ gene including 2.8kb of 5' flanking sequences and 0.13kb of 3' sequences and lacks the tissue-specific bristle and tarsal claw enhancers. (Roseman et al., 1995) 5.2kb y gene fragment containing 2.8kb of 5' flanking and 0.13kb of 3' flanking DNA and lacking the intron that is the location of the tissue-specific bristle and tarsal claw enhancers. (Roseman et al., 1995) 5kb intronless SalI fragment containing the y gene. (Harrison and Perrimon, 1993) 5.2kb SalI y gene intronless fragment containing 2.9kb of 5' flanking and 0.3kb of 3' flanking DNA. (Patton et al., 1992)
Phenotypic class	body color defective (Ring and Garza, 2003.6.10, Roseman et al., 2001) visible (Roseman et al., 2001)
Phenotype manifest in	adult cuticle (Roseman et al., 2001) arista (Ring and Garza, 2003.6.10) macrochaeta (Ring and Garza, 2003.6.10) unguis (Ring and Garza, 2003.6.10)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference Scer\GAL4-targeted reporter; β-galactosidase distribution reflects action of enhancer/promoter elements driving GAL4 in the same cell.Product localized to microtubules: carries binding domain of Btau\MAPT microtubule-associated protein. (O'Keefe et al., 1998) Scer\GAL4-targeted reporter: expression of Ecol\lacZ reflects action of enhancer/promoter elements driving GAL4 in the same cell.Product localized to microtubules; allows visualization of cell body, axons and other projections. (O'Keefe et al., 1998)

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	pP{etau-lacZ} (Lee and Luo, 1999) pP{UAST} (Lee and Luo, 1999) pP{YES} (Lee and Luo, 1999)
descendant(s)
Comments
	construct_comment: The su(Hw)-binding regions (gypsy\su(Hw)BR) act to buffer inserted sequences from chromosomal position effects.Progenitors are those of the plasmid, pP{UAS-lacZ.btau.YES}. (Lee and Luo, 1999) report comment: β-gal localizes to microtubules; facilitates visualization of neural projections. (Lee and Luo, 1999)
Stocks Listed in FlyBase ( 2 )
Bloomington	5148 y¹ w^*; P{UAS-lacZ.btau.YES}LL8/CyO
Kyoto	108079 y¹ w^*; P{UAS-lacZ.btau.YES}LL8/CyO
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{UAS-lacZ.btau.YES} (FlyBase, 1996-) P^UAS-tau-lacZ (Battye et al., 1999) tau-LacZ (Srahna et al., 2006) UAS-tau.lacZ (Hassan et al., 2000) UAS-tau-lacZ (Suster et al., 2003, Gao et al., 2000) UAS-tlacZ (Simpson et al., 2000)
Secondary FlyBase IDs

References ( 10 )
Research paper	Srahna et al., 2006, PLoS Biol. 4(11): e348 A signaling network for patterning of neuronal connectivity in the Drosophila brain. [FBrf0192599] Couch et al., 2004, Development 131(5): 997--1006 robo2 and robo3 interact with eagle to regulate serotonergic neuron differentiation. [FBrf0174592] Suster et al., 2003, J. Neurobiol. 55(2): 233--246 Targeted expression of tetanus toxin reveals sets of neurons involved in larval locomotion in Drosophila. [FBrf0159050] Gao et al., 2000, Nat. Neurosci. 3(8): 780--785 Convergent projections of Drosophila olfactory neurons to specific glomeruli in the antennal lobe. [FBrf0128472] Hassan et al., 2000, Neuron 25(3): 549--561 atonal regulates neurite arborization but does not act as a proneural gene in the Drosophila brain. [FBrf0127108] Hitier et al., 2000, Mech. Dev. 99(1-2): 93--100 no-bridge and linotte act jointly at the interhemispheric junction to build up the adult central brain of Drosophila melanogaster. [FBrf0131314] Simpson et al., 2000, Cell 103(7): 1019--1032 Short-range and long-range guidance by Slit and its Robo receptors: a combinatorial code of Robo receptors controls lateral position. [FBrf0132257] van Meyel et al., 2000, Development 127(9): 1823--1831 Chip is an essential cofactor for Apterous in the regulation of axon guidance in Drosophila. [FBrf0127419] Battye et al., 1999, Development 126(11): 2475--2481 Axon repulsion from the midline of the Drosophila CNS requires slit function. [FBrf0108520] Lee and Luo, 1999, Neuron 22(3): 451--461 Mosaic analysis with a repressible neurotechnique cell marker for studies of gene function in neuronal morphogenesis. [FBrf0107846]